In vitro development of 'Ouro' banana after polyploidization with antimitotics

Abstract:

The objective of this work was to characterize the in vitro development of 'Ouro' banana after polyploidization with antimitotics. Shoot apexes were subjected to the following treatments for 24 and 48 hours: amiprophos-methyl (APM), at 0, 10, 20, 30, 40, and 60 μmol L^-1; caffeine, at 3, 6, 9, and 12 g L^-1; and colchicine, at 2.5 mmol L^-1. Survival, number of shoots, main shoot height, and number of roots were evaluated. The intermediary concentrations were the most promising at both exposure times for APM and at 24 hours for caffeine. The highest concentrations of APM and caffeine negatively affect the in vitro development of the shoot apexes.

Index terms: Musa acuminata; amiprophos-methyl; caffeine; colchicine