Efeitos de um biocida comercial, casugamicina e consistência do meio de cultura no estabelecimento <i>in vitro</i> de bambu

Santos, Daniela Werner Ribeiro dos; Rocker, Théo Piucco; Ornellas, Thiago Sanches; Guerra, Miguel Pedro

Daniela Werner Ribeiro dos Santos E-mail/ORCID: wernerdwr@gmail.com/0000-0002-4112-8954, theopr1997@gmail.com/0000-0003-0542-3486, thisorn@gmail.com/0000-0002-6638-0937.

Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Departamento de Fitotecnia, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Florianópolis, SC, Brasil.

https://orcid.org/0000-0002-4112-8954

Théo Piucco Rocker E-mail/ORCID: wernerdwr@gmail.com/0000-0002-4112-8954, theopr1997@gmail.com/0000-0003-0542-3486, thisorn@gmail.com/0000-0002-6638-0937.

Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Departamento de Fitotecnia, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Florianópolis, SC, Brasil.

https://orcid.org/0000-0003-0542-3486

Thiago Sanches Ornellas E-mail/ORCID: wernerdwr@gmail.com/0000-0002-4112-8954, theopr1997@gmail.com/0000-0003-0542-3486, thisorn@gmail.com/0000-0002-6638-0937.

Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Departamento de Fitotecnia, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Florianópolis, SC, Brasil.

https://orcid.org/0000-0002-6638-0937

Miguel Pedro Guerra E-mail/ORCID: wernerdwr@gmail.com/0000-0002-4112-8954, theopr1997@gmail.com/0000-0003-0542-3486, thisorn@gmail.com/0000-0002-6638-0937.E-mail/ORCID: miguel.guerra@ufsc.br/0000-0002-5319-3446.

Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Departamento de Fitotecnia, Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Florianópolis, SC, Brasil.

Universidade Federal de Santa Catarina, Campus de Curitibanos, Programa de Pós-Graduação em Ecossistemas Agrícolas e Naturais, Curitibanos, SC, Brasil.

https://orcid.org/0000-0002-5319-3446

E-mail/ORCID: wernerdwr@gmail.com/0000-0002-4112-8954, theopr1997@gmail.com/0000-0003-0542-3486, thisorn@gmail.com/0000-0002-6638-0937.

E-mail/ORCID: miguel.guerra@ufsc.br/0000-0002-5319-3446.

Factor	^{_________________________________} Spring ^{_________________________________}			^{______________________________} Autumn ^{______________________________}
Factor	Oxidation (%)	Contamination (%)	Establishment (%)	Oxidation (%)	Contamination (%)	Establishment (%)
PPM^TM (mL L^-1) (P): means¹ 1 Means followed by the same letter in the same column and factor do not differ statistically according to the Duncan test at 5 % of probability. PPMTM: Plant Preservative Mixture.
0	51.7 ± 7.4 a	57.5 ± 5.7 a	10.8 ± 4.6 a	1.7 ± 1.7 a	75.0 ± 6.7 a	22.5 ± 6.6 b
2	66.7 ± 5.4 a	34.2 ± 6.3 a	10.8 ± 4.6 a	6.7 ± 3.1 a	40.0 ± 6.9 b	53.3 ± 7.3 a
4	61.7 ± 7.4 a	40.8 ± 6.1 a	12.5 ± 5.2 a	6.7 ± 3.9 a	29.2 ± 6.5 b	50.0 ± 7.2 a
Kasugamycin (K): means¹ 1 Means followed by the same letter in the same column and factor do not differ statistically according to the Duncan test at 5 % of probability. PPMTM: Plant Preservative Mixture.
Absent	52.2 ± 4.9 b	46.7 ± 5.6 a	12.8 ± 3.9 a	4.4 ± 2.1 a	58.9 ± 6.4 a	33.9 ± 6.6 b
Present	67.8 ± 5.6 a	41.7 ± 4.8 a	10.0 ± 3.9 a	5.5 ± 2.8 a	37.2 ± 6.0 b	50.0 ± 5.6 a
Consistency of the culture medium (M): means¹ 1 Means followed by the same letter in the same column and factor do not differ statistically according to the Duncan test at 5 % of probability. PPMTM: Plant Preservative Mixture.
Liquid	63.3 ± 5.6 a	50.5 ± 5.4 a	5.5 ± 2.8 b	7.8 ± 3.1 a	34.4 ± 7.0 b	55.5 ± 6.9 a
Solid	56.7 ± 5.3 a	37.8 ± 4.8 a	17.2 ± 4.5 a	2.2 ± 1.5 a	61.7 ± 4.8 a	28.3 ± 4.2 b
Interactions: F-test
P x K	2.16^ns	1.82^ns	1.56^ns	0.15^ns	0.56^ns	4.96*
P x M	0.13^ns	0.59^ns	0.06^ns	1.34^ns	0.92^ns	1.13^ns
K x M	1.44^ns	1.05^ns	0.84^ns	0.10^ns	3.34^ns	3.31^ns
P x K x M	2.16^ns	0.93^ns	0.06^ns	0.15^ns	0.92^ns	0.76^ns

Factor	^{_________________________________} Spring ^{_________________________________}			^{_______________________________} Autumn ^{_______________________________}
Factor	Oxidation (%)	Contamination (%)	Establishment (%)	Oxidation (%)	Contamination (%)	Establishment (%)
PPM^TM (mL L^-1) (P): means¹ 1 Means followed by the same letter in the same column and factor do not differ statistically according to the Duncan test at 5 % of probability. PPMTM: Plant Preservative Mixture.
0	28.3 ± 6.6 a	26.7 ± 6.2 a	41.7 ± 7.9 b	0.0 ± 0.0 a	63.3 ± 5.3 a	35.0 ± 5.7 b
2	30.0 ± 6.8 a	26.7 ± 6.2 a	46.7 ± 7.4 ab	5.0 ± 3.6 a	45.0 ± 5.0 b	43.3 ± 4.3 b
4	25.0 ± 6.3 a	10.0 ± 4.3 b	63.3 ± 5.9 a	1.7 ± 1.7 a	31.7 ± 8.2 b	60.0 ± 8.6 a
Kasugamycin (K): means¹ 1 Means followed by the same letter in the same column and factor do not differ statistically according to the Duncan test at 5 % of probability. PPMTM: Plant Preservative Mixture.
Absent	20.0 ± 4.7 b	27.8 ± 5.3 a	47.8 ± 5.7 a	1.1 ± 1.1a	50.0 ± 5.2 a	48.9 ± 5.2 a
Present	35.5 ± 5.5 a	14.4 ± 5.5 b	53.3 ± 6.3 a	3.3 ± 2.4a	43.3 ± 6.0 a	43.3 ± 5.8a
Consistency of the culture medium (M): means¹ 1 Means followed by the same letter in the same column and factor do not differ statistically according to the Duncan test at 5 % of probability. PPMTM: Plant Preservative Mixture.
Liquid	40.0 ± 5.1 a	22.2 ± 4.3 a	37.8 ± 4.9 b	1.1 ± 1.1 a	38.9 ± 6.0 b	56.7 ± 6.0 a
Solid	15.5 ± 4.4 b	20.0 ± 5.2 a	63.3 ± 6.0 a	3.3 ± 2.4 a	54.4 ± 4.9 a	35.5 ± 4.2 b
Interactions: F-test
P x K	1.55^ns	0.72^ns	0.03^ns	0.25^ns	1.47^ns	0.07^ns
P x M	1.55^ns	0.72^ns	0.27^ns	0.25^ns	31.02***	45.56***
K x M	0.11^ns	0.48^ns	0.50^ns	2.65^ns	0.62^ns	5.88*
P x K x M	1.55^ns	1.61^ns	0.03^ns	0.25^ns	2.88^ns	1.82^ns

Factor	^{_________________________________} Spring ^{_________________________________}			^{_______________________________} Autumn ^{_______________________________}
Factor	Oxidation (%)	Contamination (%)	Establishment (%)	Oxidation (%)	Contamination (%)	Establishment (%)
PPM^TM (mL L^-1) (P): means¹ 1 Means followed by the same letter in the same column and factor do not differ statistically according to the Duncan test at 5 % of probability. PPMTM: Plant Preservative Mixture.
0	20.0 ± 7.0 b	56.7 ± 4.8 a	25.0 ± 5.6 a	13.3 ± 5.1 a	61.7 ± 7.7 a	16.7 ± 5.1 b
2	37.1 ± 8.9 ab	29.7 ± 5.9 b	13.1 ± 5.5 a	8.3 ± 3.3 a	41.7 ± 7.2 b	28.3 ± 6.1 ab
4	50.0 ± 8.0 a	33.3 ± 7.7 b	20.0 ± 5.3 a	16.7 ± 5.1 a	38.3 ± 7.7 b	31.7 ± 5.1 a
Kasugamycin (K): means¹ 1 Means followed by the same letter in the same column and factor do not differ statistically according to the Duncan test at 5 % of probability. PPMTM: Plant Preservative Mixture.
Absent	30.0 ± 6.3 a	50.0 ± 5.5 a	20.0 ± 4.7 a	13.3 ± 4.1 a	40.0 ± 6.7 b	32.2 ± 4.9 a
Present	41.7 ± 6.8 a	29.8 ± 4.9 b	19.1 ± 4.3 a	12.2 ± 3.4 a	54.4 ± 5.9 a	18.9 ± 3.8 b
Consistency of the culture medium (M): means¹ 1 Means followed by the same letter in the same column and factor do not differ statistically according to the Duncan test at 5 % of probability. PPMTM: Plant Preservative Mixture.
Liquid	23.8 ± 5.9 b	44.1 ± 5.9 a	23.9 ± 4.6 a	16.7 ± 3.8 a	31.1 ± 5.3 b	35.5 ± 4.5 a
Solid	46.7 ± 6.5 a	36.7 ± 5.0 a	15.5 ± 4.3 a	8.9 ± 3.5 a	63.3 ± 5.0 a	15.5 ± 3.8 b
Interactions: F-test
P x K	3.07^ns	1.67^ns	0.41^ns	1.01^ns	9.81**	3.12^ns
P x M	1.11^ns	0.74^ns	0.05^ns	0.00^ns	3.83^ns	3.12^ns
K x M	0.82^ns	0.00^ns	2.30^ns	0.38^ns	0.02^ns	0.00^ns
P x K x M	3.07^ns	11.86**	0.41^ns	0.25^ns	0.00^ns	0.06^ns

[1] E-mail/ORCID: wernerdwr@gmail.com/0000-0002-4112-8954, theopr1997@gmail.com/0000-0003-0542-3486, thisorn@gmail.com/0000-0002-6638-0937.

Brasil

Brasil

Efeitos de um biocida comercial, casugamicina e consistência do meio de cultura no estabelecimento in vitro de bambu

RESUMO