Diferenças nos mecanismos de resposta bioquímica, fisiológica e molecular de arroz, arroz daninho e capim-arroz submetidos a seca

Agostinetto, Dirceu; Oliveira, Cláudia; Langaro, Ana Claudia; Silva, Jéssica Dias Gomes da; Barbieri, Geovana Facco

Dirceu Agostinetto

Universidade Federal de Pelotas, Faculdade de Agronomia, Capão do Leão, RS, Brasil.

https://orcid.org/0000-0001-6069-0355

Cláudia Oliveira

Universidade Federal de Pelotas, Faculdade de Agronomia, Capão do Leão, RS, Brasil.

https://orcid.org/0000-0002-8205-6686

Ana Claudia Langaro

Universidade Federal de Pelotas, Faculdade de Agronomia, Capão do Leão, RS, Brasil.

https://orcid.org/0000-0002-6746-5692

Jéssica Dias Gomes da Silva

Universidade Federal de Pelotas, Faculdade de Agronomia, Capão do Leão, RS, Brasil.

https://orcid.org/0000-0001-6224-4025

Geovana Facco Barbieri

Universidade Federal de Pelotas, Faculdade de Agronomia, Capão do Leão, RS, Brasil.

https://orcid.org/0000-0003-0692-0206

Figuras (4)
Tabelas (2)

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Figure 1
Photosynthesis rate (A ; A), substomatal CO₂ concentration (Ci; B), stomatal conductance (gs; C), transpiration (E ; D), carboxylation efficiency (CE; E) and intrinsic water-use efficiency (iWUE; F) in rice, weedy rice and barnyardgrass under water deficit or well-watered conditions. * or ^ns: means differ or not between the water conditions in the same plant, respectively, according to the t-test (p ≤ 0.05). Letters compared different plants in the same hydric treatment, according to the Duncan test (p ≤ 0.05).

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Figure 2
Roots dry matter (RDM; A), protein (B), hydrogen peroxide (H₂O₂; C) and thiobarbituric acid reactive substance (TBARS; D) in rice, weedy rice and barnyardgrass under water deficit or well-watered conditions at 5 days of the treatment. * or ^ns: means differ or not between the water conditions in the same plant, respectively, according to the t-test (p ≤ 0.05). Letters compared different plants in the same hydric treatment according to the Duncan test (p ≤ 0.05). FW: fresh weight; MDA: malondialdehyde.

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Figure 3
Quantification of the proline (A) and carotenoid (B) levels in well-watered or water deficit plants. * Means differ between the water conditions according to the t-test (p ≤ 0.05). FW: fresh weight.

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Figure 4
Relative expression (RQ) profiles of OsAPX2 (A), OsHSP24.15 (B), OsHSP71.10 (C) and OsHSP81.88 (D) in rice, weedy rice and barnyardgrass subjected or not to water deficit. * or ^ns: means differ or not between water conditions in the same plant, respectively, according to the t-test (p ≤ 0.05). Letters compared different plants in the same hydric treatment according to the Duncan test (p ≤ 0.05).

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Table 1
Primers used a reference and target primer for RTqPCR in rice, weedy rice and barnyardgrass in response to drought stress.

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Table 2
Activity of the enzymes catalase, ascorbate peroxide, superoxide dismutase and proline, chlorophyll a, b and total, and carotenoid in rice, weedy rice and barnyardgrass.

Gene	Forward (5’-3’)	Reverse (5’-3’)	Reference
OsUBC-E2	CCGTTTGTAGA GCCATAATTGCA	AGGTTGCCTGAG TCACAGTTAAGTG	Jain et al. 2006JAIN, M.; NIJHAWAN, A.; TYAGI, A. K.; KHURANA, J. P. Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochemical and Biophysical Research Communications, v. 345, n. 2, p. 646-651, 2006.
OsUBQ10	ACCACTTCGAC CGCCACTACT	ACGCCTAAGC CTGCTGGTT	Jain et al. 2006JAIN, M.; NIJHAWAN, A.; TYAGI, A. K.; KHURANA, J. P. Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochemical and Biophysical Research Communications, v. 345, n. 2, p. 646-651, 2006.
OsAPX2	AGAGTCAGT ACGATCAAGAC	TCTTGACAGC AAATAGCTTGG	Zhang et al. 2014ZHANG, J. J.; LU, Y. C.; ZHANG, J. J. Accumulation and toxicological response of atrazine in rice crops. Ecotoxicology and Environmental Safety, v. 102, n. 1, p. 105-112, 2014.
OsHsp24.15	GATCAAGGCG GAGATGAAGAAC	ACTCGACGTT GACCTGGAAGA	Ye et al. 2012YE, S. F.; YU, S. W.; SHU, L. B.; WU, J. H.; WU, A. Z.; LUO, L. J. Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice. Chinese Science Bulletin, v. 57, n. 4, p. 336-346, 2012.
OsHsp71.10	CCGTGTGCTT CGACATTGAC	CGTTGGTGATG GTGATCTTGTT	Ye et al. 2012YE, S. F.; YU, S. W.; SHU, L. B.; WU, J. H.; WU, A. Z.; LUO, L. J. Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice. Chinese Science Bulletin, v. 57, n. 4, p. 336-346, 2012.
OsHsp85.88	ACGGTGACGG AGGTGATTGA	AACAAAGGATGC CCAAGAGAAC	Ye et al. 2012YE, S. F.; YU, S. W.; SHU, L. B.; WU, J. H.; WU, A. Z.; LUO, L. J. Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice. Chinese Science Bulletin, v. 57, n. 4, p. 336-346, 2012.

Enzymes	Rice	Weedy rice	Barnyardgrass	CV (%)
Catalase	0.34 a¹ 1 Means followed by the same letter in the row do not differ by the Duncan test (p ≤ 0.05). FW: fresh weight; AU: activity unit.	0.24 b	0.02 c	21.31
Ascorbate peroxide	0.24 a¹ 1 Means followed by the same letter in the row do not differ by the Duncan test (p ≤ 0.05). FW: fresh weight; AU: activity unit.	0.18 a	0.08 b	22.16
Superoxide dismutase (AU mg^-1 prot min^-1)	1.64 b	1.85 b	4.51 a	19.35
Proline (μg proline g^-1 FW)	24.04 a	24.90 a	13.79 b	12.67
Chlorophyll a	2.32 a	1.71 b	1.79 b	15.32
Chlorophyll b	0.82 a	0.67 b	0.68 b	17.29
Total chlorophyll	3.15 a	2.48 b	2.47 b	15.42
Carotenoid (mg g^-1 FW)	0.62 a	0.47 b	0.32 c	16.06

[TFN1] ¹ Means followed by the same letter in the row do not differ by the Duncan test (p ≤ 0.05). FW: fresh weight; AU: activity unit.

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Diferenças nos mecanismos de resposta bioquímica, fisiológica e molecular de arroz, arroz daninho e capim-arroz submetidos a seca

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