Effect of the herbicides on the chlorophyll content of Spirodela punctata The aim of this study was to verify the effects of the herbicides butachlor, glyphosate and propanil on content of chlorophyll of aquatic plant Spirodela punctata. In order to determine the effect of different concentrations of 0.005; 0.05; 0.5 and 5 mgL-1 (active ingredient) of three herbicides on the contents of chlorophyll a, b and a/b ratio. The bioassays were conducted, for a period of seven full days, in a climatedcontrolled room. Chlorophyll content was determined by spectrophotometric absorbency readings at A645 and A663 nm. Butachlor reduced chlorophyll b, the glyphosate decrease chlorophyll a, while the propanil reduced chlorophyll a, b and a/b ratio, causing the greatest reduction of the pigments in the species.
Pigments; butachlor; propanil; glyphosate; aquatic plant