Effect of environmental factors on the germination of Megathyrsus maximus: an invasive weed in sugarcane in Argentina

Abstract

Background:

Megathyrsus maximus (Jacq.) is a perennial weed that affects many crops. In Argentina, sugarcane is the most affected.

Objective:

Study effective techniques to break dormancy and the effect of environmental factors on the germination of this weed.

Methods:

Experiments were carried out in cabinet incubators twice, with five replicates per treatment. The experimental unit was made up for 50 seeds.

Results:

Seed dormancy was strongly associated with the presence of glumes. Manual extractions of glumes and immersion in sulphuric acid were the most effective techniques for breaking dormancy. Megathyrsus maximus did not depend on light to germinate, and it did so both under a 12 h-light-dark photoperiod and in complete darkness, with maximum mean germination percentages of 73 and 76%, respectively. Mean germination percentage (G) and coefficient of germination (CG: number of germinated seeds per day) showed that this weed responded to a wide range of temperatures, the optimal varied between 25 and 35 °C. Both mean germination percentages and CG decreased as osmotic potential became increasingly negative (0 MPa to -0.6 MPa), and as sodium chloride solution concentrations increased (10 to 130 mmol L^-1). No germination was observed at -0.8 MPa and with a 150 mmol L^-1 sodium chloride solution.

Conclusions:

The optimal germination conditions for M. maximus can be found in central sugarcane areas in Argentina, since soils are in ideal conditions and are kept under irrigation. In marginal areas, M. maximus germination would depend on rainfall and certain sodium chloride concentrations in the soil.

Keywords:
Megathyrsus maximus ; guinea grass; scarification; salt stress; temperature; osmotic stress; dormancy