Knowledge of foliar herbicide washout is essential to optimize weed management and to avoid environmental problems. Bioassays to detect small quantities of herbicides in water samples are low-cost techniques extensively used in herbicide research. These methods measure the biological response of a living organism to determine the presence and concentration of a chemical in a substrate. The purpose of this paper was to quantify glyphosate and sulfosate residues in the water, after 53 mm h-1 of rainfall simulation, applied to the Brachiaria brizantha plant, grown without and under water stress. Tomato (Lycopersicon esculentum) was used as test-plant. According to the results, it may be concluded that: the I50 values for the glyphosate and sulfosate obtained in the standard curves were 324.12 and 407.86 mg L-1, respectively, for root length; the I50 was lower when the herbicides had been applied in plants under water stress.
bioassay; water stress; postemergence; foliar absorption
