Complementary quantitative analyses are necessary for several interdisciplinary studies using Plant Anatomy. Generally, micromorphometric evaluation is performed manually and/or using non-specific software for image analyses. This work aimed to develop specific quantitative analysis software for Plant Anatomy and test its efficiency and acceptance by users. The solution was elaborated in the JAVA language, which has a greater mobility in relation to the operating system to be used. The software developed was called ANATI QUANTI and tested by students, researchers and professors of the Plant Anatomy Laboratory of the Universidade Federal de Viçosa (UFV). All the interviewees received photos to measure in ANATI QUANTI and compare with the results obtained using the available software. Through previously formulated questionnaires, the volunteers pointed out the main advantages and disadvantages of the developed software in relation to the currently available software. Besides being more specific, simpler and faster than the currently available software, ANATI QUANTI is reliable, matching the interviewees expectations. However, additional resources need to be added as well as new scales to increase users range. ANATI QUANTI is already being applied in research developed by UFV users. It is a free software and as an open source, it it will be available for free over the internet.
micromorphometric analysis; Java; stomatal density; stomatal index