Specificity |
Primers (5’- 3’) |
Target |
Name |
Size (bp) |
References |
Anaplasma phagocytophilum
|
First round |
ATGAATTACAGAGAATTGCTTGTAGG |
msp4
|
MSP4AP5 |
849 |
de la Fuente et al. 2005De la Fuente J., Massung R.F., Wong S.J., Chu F.K., Lutz H., Meli M., von Loewenich F.D., Grzeszczuk A., Torina A., Caracappa S., Mangold A.J., Naranjo V., Stuen S. & Kocan K.M. 2005. Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains. J. Clin. Microbiol. 43:1309-1317.
|
TTAATTGAAAGCAAATCTTGCTCCTATG |
MSP4AP3 |
Second round |
CTATTGGYGGNGCYAGAGT |
msp4
|
msp4f |
381 |
Bown et al. 2007Bown K.J., Lambin X., Ogden N.H., Petrovec M., Shaw S.E., Woldehiwet Z. & Birtles R.J. 2007. High-resolution genetic fingerprinting of European strains of Anaplasma phagocytophilum by use of multilocus variable-number tandem-repeat analysis. J. Clin. Microbiol. 45:1771-1776.
|
GTTCATCGAAAATTCCGTGGTA |
msp4r |
Anaplasma phagocytophilum
|
ATTGGACTTTTGAGCTGTCTT |
msp2/p44
|
p44F |
1082 |
Lin et al. 2003Lin Q., Rikihisa Y., Ohashi N. & Zhi N. 2003. Mechanisms of variable p44 expression by Anaplasma phagocytophilum. Infect. Immun. 71:5650-5661.
|
CAATAGTYTTAGCTAGTAACC |
p44R |
Anaplasma phagocytophilum
|
CCAGCGTTTAGCAAGATAAGAG |
msp2/p44
|
msp2-3F |
334 |
Zeidner et al. 2000Zeidner N.S., Burkot T.R., Massung R., Nicholson W.L., Dolan M.C., Rutherford J.S., Biggerstaff B.J. & Maupin G.O. 2000. Transmission of the agent of human granulocytic ehrlichiosis by Ixodes spinipalpis ticks: evidence of an enzootic cycle of dual infection with Borrelia burgdorferi in northern Colorado. J. Infect. Dis. 182:616-619.
|
GCCCAGTAACATCATAAGC |
msp2-3R |
Granulocyte/platelet Anaplasma/Ehrlichia
|
First round |
CACATGCAAGTCGAACGGATTATTC |
GE3a |
16S rRNA |
932 |
Massung et al. 1998Massung R.F., Slater K., Owens J.H., Nicholson W.L., Mather T.N., Solberg V.B. & Olson J.G. 1998. Nested PCR assay for detection of granulocytic ehrlichiae. J. Clin. Microbiol. 36:1090-1095.
|
TTCCGTTAAGAAGGATCTAATCTCC |
GE10r |
Second round |
AACGGATTATTCTTTATAGCTTGCT |
GE9f |
16S rRNA |
546 |
Massung et al. 1998Massung R.F., Slater K., Owens J.H., Nicholson W.L., Mather T.N., Solberg V.B. & Olson J.G. 1998. Nested PCR assay for detection of granulocytic ehrlichiae. J. Clin. Microbiol. 36:1090-1095.
|
GGCAGTATTAAAAGCAGCTCCAGG |
GE2 |
Monocyte Ehrlichia spp. Lineage |
First round |
ACGGACAATTGCTTATAGCCTT |
NS16SCH1F |
16S rRNA |
1195 |
Kawahara et al. 2009Kawahara M., Tajima T., Torii H., Yabutani M., Ishii J., Harasawa M., Isogai E. & Rikihisa Y. 2009. Ehrlichia chaffeensis infection of sika deer. Japan. Emerg. Infect. Dis. 15:1991-1993.
|
ACAACTTTTATGGATTAGCTAAAT |
NS16SCH1R |
Second round |
GGGCACGTAGGTGGACTAG |
NS16SCH2F |
16S rRNA |
443 |
Kawahara et al. 2009Kawahara M., Tajima T., Torii H., Yabutani M., Ishii J., Harasawa M., Isogai E. & Rikihisa Y. 2009. Ehrlichia chaffeensis infection of sika deer. Japan. Emerg. Infect. Dis. 15:1991-1993.
|
CCTGTTAGGAGGGATACGAC |
NS16SCH2R |