Agents |
Tissue |
Primer (5´-3´) |
Reference |
Feline coronavirus (FCoV) |
Lung and cerebral cortex |
TAATGCCATACACGAACCAGCT GTGCTAGATTTGTCTTCGGACACC |
Simons et al. (2005)Simons F.A., Vennema H., Rofina J.E., Pol J.M., Horzinek M.C., Rottier P.J.M. & Egberink H.F. 2005. A mRNA PCR for the diagnosis of feline infectious peritonitis. J. Virol. Methods 124(1/2):111-116. <https://dx.doi.org/10.1016/j.jviromet.2004.11.012> <PMid:15664058> https://doi.org/10.1016/j.jviromet.2004....
|
Leishmania sp. |
Skin and subcutaneous |
(C/G)(G/C)CC(C/A)CTA T(T/A)TTACACCAACCCC GGGGAGGGGCGTTCTGCGAA |
Quaresma et al. (2009)Quaresma P.F., Murta S.M.F., Ferreira E.C., Rocha-Lima A.C.V.M., Xavier A.A.P. & Gontijo C.M.F. 2009. Molecular diagnosis of canine visceral leishmaniasis: identification of Leishmania species by PCR-RFLP and quantification of parasite DNA by real-time PCR. Acta Trop. 111(3):289-294. <https://dx.doi.org/10.1016/j.actatropica.2009.05.008> <PMid:19467216> https://doi.org/10.1016/j.actatropica.20...
|
Feline parvovirus (FPV) |
Intestine |
GGATCACCATCTGCTGCTTG GCTGAGGTTGGTTATAGTGC |
Yoon et al. (2009)Yoon S.H., Jeong W., Kim H. & An D.-J. 2009. Molecular insights into the phylogeny of canine parvovirus 2 (CPV-2) with emphasis on Korean isolates: a Bayesian approach. Arch. Virol. 154(8):1353-1360. <https://dx.doi.org/10.1007/s00705-009-0444-3> <PMid:19590812> https://doi.org/10.1007/s00705-009-0444-...
|
Feline immunodeficiency virus (FIV) |
Lung and spleen |
GCGCTAGCAGCTGCCTAACCGCAAAACCAC GTATCTGTGGGAGCCTCAAGGGAGAACTC |
Szilasi et al. (2021)Szilasi A., Dénes L., Krikó E., Murray C., Mándoki M. & Balka G. 2021. Prevalence of feline leukaemia virus and feline immunodeficiency virus in domestic cats in Ireland. Acta Vet. Hungarica 68(4):413-420. <https://dx.doi.org/10.1556/004.2020.00056> <PMid:33459612> https://doi.org/10.1556/004.2020.00056...
|
Feline leukemia virus (FeLV) |
Lung and lymph node |
GCTCCCCAGTTGACCAGAGT TTTATAGCAGAAAGCGCGCG |
Duda et al. (2020)Duda N.C.B., Ortiz L.C., Valle S.F., Costa F.V.A., Varela A.P.M., Nunes N.J.S., Okano F.Y., Franco A.C., Roehe P.M. & González F.H.D. 2020. Laboratory and clinical findings and their association with viral and proviral loads in cats naturally infected with feline leukemia virus. Comp. Immunol. Microbiol. Infect. Dis. 71:101491. <https://dx.doi.org/10.1016/j.cimid.2020.101491> <PMid:32450457> https://doi.org/10.1016/j.cimid.2020.101...
|
Feline calicivirus virus (FCV) |
Lungs |
GTAAAAGAAATTTGAGACAAT TACTGAAGWTCGCGYCT FAM-CAAACTCTGAGCTTCGTGCTTAAA-BHQ |
Helps et al. (2005)Helps C.R., Lait P., Damhuis A., Björnehammar U., Bolta D., Brovida C., Chabanne L., Egberink H., Ferrand G., Fontbonne A., Pennisi M.G., Gruffydd-Jones T., Gunn-Moore D., Hartmann K., Lutz H., Malandain E., Möstl K., Stengel C., Harbour D.A. & Graat E.A.M. 2005. Factors associated with upper respiratory tract disease caused by feline herpesvirus, feline calicivirus, Chlamydophila felis and Bordetella bronchiseptica in cats: experience from 218 European catteries. Vet. Rec. 156(21):669-677. <https://dx.doi.org/10.1136/vr.156.21.669> <PMid:15908495> https://doi.org/10.1136/vr.156.21.669...
|
Felid alphaherpesvirus (FeHV) |
Lungs |
TGGTGCCTATGGAATAGGTAAGAGTT GTCGATTTTCATCCGCTCTGA HEX-AACGGCGAAGTACC-MGB |
Helps et al. (2003)Helps C., Reeves N., Egan K., Howard P. & Harbour D. 2003. Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis. J. Clin. Microbiol. 41(6):2734-2736. <https://dx.doi.org/10.1128/JCM.41.6.2734-2736.2003> <PMid:12791917> https://doi.org/10.1128/JCM.41.6.2734-27...
|
Chlamydia sp. |
Lungs |
CCGCCAACACTGGGACT GGAGTTAGCCGGTGCTTCTTTAC FAM-CTACGGGAGGCTGCAGTCGAGAATC-BHQ1 |
Lienard et al. (2011)Lienard J., Croxatto A., Aeby S., Jaton K., Posfay-Barbe K., Gervaix A. & Greub G. 2011. Development of a new chlamydiales-specific real-time PCR and its application to respiratory clinical samples. J. Clin. Microbiol. 49(7):2637-2642. <https://dx.doi.org/10.1128/JCM.00114-11> <PMid:21562107> https://doi.org/10.1128/JCM.00114-11...
|
Bordetella sp. |
Lungs |
ACTATACGTCGGGAAATCTGTTTG CGTTGTCGGCTTTCGTCTG FAM-CGGGCCGATAGTCAGGGCGTAG-BHQ1 |
Helps et al. (2005)Helps C.R., Lait P., Damhuis A., Björnehammar U., Bolta D., Brovida C., Chabanne L., Egberink H., Ferrand G., Fontbonne A., Pennisi M.G., Gruffydd-Jones T., Gunn-Moore D., Hartmann K., Lutz H., Malandain E., Möstl K., Stengel C., Harbour D.A. & Graat E.A.M. 2005. Factors associated with upper respiratory tract disease caused by feline herpesvirus, feline calicivirus, Chlamydophila felis and Bordetella bronchiseptica in cats: experience from 218 European catteries. Vet. Rec. 156(21):669-677. <https://dx.doi.org/10.1136/vr.156.21.669> <PMid:15908495> https://doi.org/10.1136/vr.156.21.669...
|
Mycoplasma felis
|
Lungs |
GTG GGG ATG GAT TAC CTC GGA CTA TTA TCA AAA GCA CAT AAA C HEX-CTA CGG AGT ACA AGT TAC AAT TCA-BHQ1 |
Bennett (2015)Bennett S. 2015. Development of multiplex real-time PCR screens for the diagnosis of feline and canine infectious disease. Master’s Thesis, University of Glasgow, Glasgow. 141p. Available at <Available at https://theses.gla.ac.uk/5971/ > Accessed on September 20, 2023. https://theses.gla.ac.uk/5971/...
|
Pasteurella sp. |
Lungs |
ATCCGCTATTTACCCAGTGGGCTGTAAACGAACTCGCCAC |
Townsend et al. (2001)Townsend K.M., Boyce J.D., Chung J.Y., Frost A.J. & Adler B. 2001. Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system. J. Clin. Microbiol. 39(3):924-929. <https://dx.doi.org/10.1128/JCM.39.3.924-929.2001> <PMid:11230405> https://doi.org/10.1128/JCM.39.3.924-929...
|