Organisms |
Primer name |
Primer sequence (5′-3′) |
Annealing temperature (°C) |
Expected size (bp) |
Reference |
Staphylococcus aureus
|
nuc_F |
GGTTCTGAAGATCCAACAGTAT |
61 |
296 |
Acosta et al. (2017)Acosta A.C., Santos S.J., Albuquerque L., Soares K.D.A., Mota R.A. & Medeiros E.S. 2017. Frequência de genes codificadores de toxinas em Staphylococcus aureus isolados de leite de tanques expansão comunitários. Pesq. Vet. Bras. 37(7):691-696. <https://dx.doi.org/10.1590/S0100-736X2017000700007> https://doi.org/10.1590/S0100-736X201700...
|
nuc_R |
GCTAAGCCACGTCCATATTTA |
Staphylococcus hyicus
|
hy-F1 |
CATTATATGATTTGAACGTG |
56 |
793 |
Sasaki et al. (2010)Sasaki T., Tsubakishita S., Tanaka Y., Sakusabe A., Ohtsuka M., Hirotaki S., Kawakami T., Fukata T. & Hiramatsu K. 2010. Multiplex-PCR method for species identification of coagulase-positive staphylococci. J. Clin. Microbiol. 48(3):765-769. <https://dx.doi.org/10.1128/JCM.01232-09> <PMid:20053855> https://doi.org/10.1128/JCM.01232-09...
|
hy-R1 |
GAATCAATATCGTAAAGTTGC |
Staphylococcus simulans
|
SSMF |
AGCTTCGTTTACTTCTTCGATTGT |
60 |
472 |
Shome et al. (2011)Shome B.R., Das Mitra S., Bhuvana M., Krithiga N., Velu D., Shome R., Isloor S., Barbuddhe S.B. & Rahman H. 2011. Multiplex PCR assay for species identification of bovine mastitis pathogens. J. Appl. Microbiol. 111(6):1349-1356. <https://dx.doi.org/10.1111/j.1365-2672.2011.05169.x> <PMid:21972842> https://doi.org/10.1111/j.1365-2672.2011...
|
SSMR |
AAAAGCACAAGCTCACATTGAC |
Staphylococcus epidermidis
|
SERF |
AAGAGCGTGGAGAAAAGTATCAAG |
60 |
130 |
Shome et al. (2011)Shome B.R., Das Mitra S., Bhuvana M., Krithiga N., Velu D., Shome R., Isloor S., Barbuddhe S.B. & Rahman H. 2011. Multiplex PCR assay for species identification of bovine mastitis pathogens. J. Appl. Microbiol. 111(6):1349-1356. <https://dx.doi.org/10.1111/j.1365-2672.2011.05169.x> <PMid:21972842> https://doi.org/10.1111/j.1365-2672.2011...
|
SERR |
TCGATACCATCAAAAAGTTGG |
Staphylococcus chromogenes
|
SCHS1F |
GCGTACCAGAAGATAAACAAACTC |
60 |
222 |
Shome et al. (2011)Shome B.R., Das Mitra S., Bhuvana M., Krithiga N., Velu D., Shome R., Isloor S., Barbuddhe S.B. & Rahman H. 2011. Multiplex PCR assay for species identification of bovine mastitis pathogens. J. Appl. Microbiol. 111(6):1349-1356. <https://dx.doi.org/10.1111/j.1365-2672.2011.05169.x> <PMid:21972842> https://doi.org/10.1111/j.1365-2672.2011...
|
SCHS1R |
CATTATTTACAACGAGCCATGC |
Staphylococcus haemolyticus
|
SHS1F |
CAAATTAAATTCTGCAGTTGAGG |
60 |
214 |
Shome et al. (2011)Shome B.R., Das Mitra S., Bhuvana M., Krithiga N., Velu D., Shome R., Isloor S., Barbuddhe S.B. & Rahman H. 2011. Multiplex PCR assay for species identification of bovine mastitis pathogens. J. Appl. Microbiol. 111(6):1349-1356. <https://dx.doi.org/10.1111/j.1365-2672.2011.05169.x> <PMid:21972842> https://doi.org/10.1111/j.1365-2672.2011...
|
SHS1R |
AGAGCCCCATTGTTCTTTGA |
Staphylococcus xylosus
|
Xyl F |
AACGCGCAACGTGATAAAATTAATG |
55 |
539 |
Morot-Bizot et al. (2004)Morot-Bizot S.C., Talon R. & Leroy S. 2004. Development of a multiplex PCR for the identification of Staphylococcus genus and four staphylococcal species isolated from food. J. Appl. Microbiol. 97(5):1087-1094. <https://dx.doi.org/10.1111/j.1365-2672.2004.02399.x> <PMid:15479426> https://doi.org/10.1111/j.1365-2672.2004...
|
Xyl R |
AACGCGCAACAGCAATTACG |
Staphylococcus pseudintermedius
|
pse-F2 |
TRGGCAGTAGGATTCGTTAA |
56 |
926 |
Sasaki et al. (2010)Sasaki T., Tsubakishita S., Tanaka Y., Sakusabe A., Ohtsuka M., Hirotaki S., Kawakami T., Fukata T. & Hiramatsu K. 2010. Multiplex-PCR method for species identification of coagulase-positive staphylococci. J. Clin. Microbiol. 48(3):765-769. <https://dx.doi.org/10.1128/JCM.01232-09> <PMid:20053855> https://doi.org/10.1128/JCM.01232-09...
|
pse-R5 |
CTTTTGTGCTYCMTTTTGG |
Streptococcus agalactiae
|
STAGF |
GCTAATACCGCATAAGAGTAATTAAC |
60 |
317 |
Shome et al. (2011)Shome B.R., Das Mitra S., Bhuvana M., Krithiga N., Velu D., Shome R., Isloor S., Barbuddhe S.B. & Rahman H. 2011. Multiplex PCR assay for species identification of bovine mastitis pathogens. J. Appl. Microbiol. 111(6):1349-1356. <https://dx.doi.org/10.1111/j.1365-2672.2011.05169.x> <PMid:21972842> https://doi.org/10.1111/j.1365-2672.2011...
|
STAGR |
GGTAGATTTTCCACTCCTACCAA |
Streptococcus dysgalactiae
|
STDGF |
GGGAGTGGAAAATCCACCAT |
60 |
572 |
Shome et al. (2011)Shome B.R., Das Mitra S., Bhuvana M., Krithiga N., Velu D., Shome R., Isloor S., Barbuddhe S.B. & Rahman H. 2011. Multiplex PCR assay for species identification of bovine mastitis pathogens. J. Appl. Microbiol. 111(6):1349-1356. <https://dx.doi.org/10.1111/j.1365-2672.2011.05169.x> <PMid:21972842> https://doi.org/10.1111/j.1365-2672.2011...
|
STDGR |
AAGGGAAAGCCTATCTCTAGACC |
Streptococcus uberis
|
Su-F |
AATTGGCATTCGTCGCGGTA |
53 |
239 |
Ashraf et al. (2017)Ashraf A., Imran M., Yaqub T., Tayyab M., Shehzad W. & Thomson P.C. 2017. A novel multiplex PCR assay for simultaneous detection of nine clinically significant bacterial pathogens associated with bovine mastitis. Mol. Cell. Probes 33:57-64. <https://dx.doi.org/10.1016/j.mcp.2017.03.004> <PMid:28336361> https://doi.org/10.1016/j.mcp.2017.03.00...
|
Su-R |
GCATCCCTTCAACCACTTCAA |
Streptococcus parauberis
|
Spa 301 |
GCG ACG TGG GAT CAA ATA CT |
57 |
918 |
Riffon et al. (2001)Riffon R., Sayasith K., Khalil H., Dubreuil P., Drolet M. & Lagacé J. 2001. Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. J. Clin. Microbiol. 39(7):2584-2589. <https://dx.doi.org/10.1128/JCM.39.7.2584-2589.2001> <PMid:11427573> https://doi.org/10.1128/JCM.39.7.2584-25...
|
Spa 1219 |
TAC CAT TAC CTC TAA AGG TA |
Corynebacterium bovis
|
C_bo_F |
GGTGTGGGGATCTTCCACGAT |
60 |
224 |
Kim et al. (2014)Kim T.-H., Kim D.-S., Han J.-H., Chang S.-N., Kim K.-S., Seok S.-H., Kim D.-J., Park J.-H. & Park J.-H. 2014. Detection of Corynebacterium bovis infection in athymic nude mice from a research animal facility in Korea. J. Vet. Sci. 15(4):583-586. <https://dx.doi.org/10.4142/jvs.2014.15.4.583> <PMid:24962412> https://doi.org/10.4142/jvs.2014.15.4.58...
|
C_bo_R |
ACCACCTGTGAACAAGCCCA |
Corynebacterium pseudotuberculosis
|
PLD-F |
ATAAGCGTAAGCAGGGAGCA |
58 |
203 |
Pacheco et al. (2007)Pacheco L.G.C., Pena R.R., Castro T.L.P., Dorella F.A., Bahia R.C., Carminati R., Frota M.N.L., Oliveira S.C., Meyer R., Alves F.S.F., Miyoshi A. & Azevedo V. 2007. Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples. J. Clin. Microbiol. 56(Pt 4):480-486. <https://dx.doi.org/10.1099/jmm.0.46997-0> <PMid:17374887> https://doi.org/10.1099/jmm.0.46997-0...
|
PLD-R1 |
ATCAGCGGTGATTGTCTTCC |
Escherichia coli
|
ECPF |
GGTAACGTTTCTACCGCAGAGTTG |
60 |
468 |
Shome et al. (2011)Shome B.R., Das Mitra S., Bhuvana M., Krithiga N., Velu D., Shome R., Isloor S., Barbuddhe S.B. & Rahman H. 2011. Multiplex PCR assay for species identification of bovine mastitis pathogens. J. Appl. Microbiol. 111(6):1349-1356. <https://dx.doi.org/10.1111/j.1365-2672.2011.05169.x> <PMid:21972842> https://doi.org/10.1111/j.1365-2672.2011...
|
ECPR |
CAGGGTTGGTACACTGTCATTACG |
Klebsiella pneumoniae
|
SHV-F |
GCTGGCGGTACACGCCAGCCCG |
55 |
995 |
Fonseca et al. (2017)Fonseca E.L., Ramos N.d.V., Andrade B.G.N., Morais L.L.C.S., Marin M.F.A. & Vicente A.C.P. 2017. A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine. Diagn. Microbiol. Infect. Dis. 87(4):315-317. <https://dx.doi.org/10.1016/j.diagmicrobio.2017.01.005> <PMid:28139276> https://doi.org/10.1016/j.diagmicrobio.2...
|
DeoR-R |
AGAAGCATCCTGCTGTGCG |