Evolução das enfermidades tóxicas em bovinos na região sul do Rio Grande do Sul: distribuição espacial e tendência em 42 anos

pvb Pesquisa Veterinária Brasileira Pesq. Vet. Bras. 0100-736X 1678-5150 Colégio Brasileiro de Patologia Animal - CBPA RESUMO: Na região sul do Rio Grande do Sul, dentre as principais enfermidades que causam mortalidade em bovinos estão diversas doenças tóxicas, destacando-se as intoxicações por plantas e micotoxicoses. O objetivo deste trabalho foi realizar um estudo comparativo para determinar a evolução das principais intoxicações que ocorreram em bovinos na região sul do Rio Grande do Sul de 1979 a 1999 e de 2000 a 2020, estabelecendo a distribuição espacial e a tendência de ocorrência dessas intoxicações nos 42 anos de atividades do Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal de Pelotas (LRD-UFPel), possibilitando prever sua ocorrência nas próximas décadas. Foram recebidos no laboratório de janeiro de 1979 a dezembro de 1999 e de janeiro de 2000 a dezembro de 2020, 3.753 e 3.653 materiais de bovinos para diagnóstico, respectivamente. Do total de materiais recebidos, 392 foram diagnosticados como intoxicações, sendo 140 entre os anos de 1979 a 1999 e 252 casos de 2000 a 2020. Dos 140 casos diagnosticados nos primeiros 21 anos de funcionamento do LRD-UFPel, 113 (80,7%) foram intoxicações por plantas, 22 (15,7%) intoxicações por fungos e cinco (3.6%) intoxicações por substâncias químicas. Entre os anos de 2000 e 2020 dos 252 casos diagnosticados 224 (88,8%) foram por plantas tóxicas, 10 (3,9%) por fungos, 12 (4,8%) por substâncias químicas e seis (2,4%) foram por insetos. Na análise de tendência temporal foi observada uma tendência linear significativa com valores de p=0,003 e mudança percentual anual (APC) de 2,5 no crescimento do número de diagnósticos de intoxicações em geral ao longo de todo o período de funcionamento do LRD-UFPel. Quando analisada a tendência temporal da intoxicação por Senecio spp. houve aumento na ocorrência do diagnóstico, com características e pontos de inflexão diferentes ao longo do tempo, sendo observado crescimento anual de 9% nos diagnósticos durante os primeiros 21 anos. Conclui-se que houve queda na ocorrência das intoxicações por Solanum fastigiatum, Echium plantagineum e Claviceps paspali e que as intoxicações por Ramaria flavo-brunnescens e Baccharis coridifolia mantiveram um percentual semelhante em todo o período. Além disso, as doenças tóxicas provavelmente permanecerão como importantes causas de morte de bovinos na região apesar de bem conhecidas, devido em parte, às dificuldades em fazer o controle das mesmas. Introduction In Brazil, a country of continental dimensions with five different biomes, there are countless different plant genera and species that cause disease and death in cattle. Due to extensive breeding, plant poisoning is one of the main causes of cattle mortality and economic losses throughout the country. These poisonings have been frequently studied in recent decades, and some toxic plant species and genera are well-known in their regions of occurrence (Rissi et al. 2007, Pessoa et al. 2013). However, some poisonings by plants or fungi do not occur every year due to climatic conditions or other factors and end up being neglected in terms of diagnosis by farmers and veterinarians, which can lead to considerable economic losses when they occur again after a period of time (Riet-Correa & Medeiros 2001, Rissi et al. 2007, Lucena et al. 2010). Poisoning by Baccharis coridifolia, known since the colonization of South America (Cobo 1964, Almeida et al. 2013), and Ramaria flavo-brunnescens, known since the 1950s (Barros 1958, Freitas et al. 1966), are clear examples of the importance of identifying these etiological agents, knowing their toxic principles and/or modes of action to prevent outbreaks. Despite the vast literature and studies related to intoxications in cattle in Brazil (Tokarnia et al. 1979, 2000, 2012, Riet-Correa et al. 1983, Rissi et al. 2007, Pessoa et al. 2013), research on the evolution of these intoxications and the trend of occurrence and spatial distribution in a particular region is needed to establish the biological behavior of toxic agents in order to predict and prevent these intoxications. It is possible that changes in and/or the emergence of large soybean fields or transgenic crops may also influence the expansion of toxic species (Monquero 2005). The objective of the study was to carry out a comparative analysis to determine the evolution of the main cattle intoxications occurring in southern Rio Grande do Sul State from 1979 to 1999 and from 2000 to 2020. Our aim was to establish the spatial distribution and occurrence trend of these intoxications in the 42 years of activities of the “Laboratório Regional de Diagnóstico” (Regional Diagnostic Laboratory) of the “Faculdade de Veterinária” (Faculty of Veterinary Medicine) at “Universidade Federal de Pelotas” (LRD-UFPel) to predict their occurrence in the coming decades. Materials and Methods A retrospective study of cattle poisonings diagnosed at the “Laboratório Regional de Diagnóstico” of the “Faculdade de Veterinária” at “Universidade Federal de Pelotas” (LRD-UFPel) was carried out. For this study, cattle necropsies or organ protocols sent for histopathological analysis from January 1979 to December 1999 and from January 2000 to December 2020 were reviewed. The various diagnoses were divided according to the etiological agent into poisoning by plants, fungi/mushrooms, insects, and chemical substances. The different intoxications were grouped in each study period, and data on morbidity, mortality, lethality, time of year of occurrence, animal age, and number of outbreaks/cases were extracted. A temporal analysis of the occurrence trend of poisoning by Senecio spp. and other poisonings was conducted to assess their patterns with regard to the number of outbreaks over time. Temporal trends were calculated based on animal incidence rates using joinpoint regression models (Joinpoint Regression Program version 4.8.0.1). Temporal trends were examined by segmented linear regression, based on the calculation of annual percentage changes (APCs) and average annual percentage changes (AAPCs), with their respective 95% confidence intervals (95% CIs). APCs and AAPCs were considered significant when p<0.05 and their 95% CIs did not include zero. The results were interpreted as follows: positive and significant APCs/AAPCs were considered increasing trends in the occurrence of intoxications, negative and significant APCs/AAPCs were considered decreasing trends in the occurrence of intoxications, and in cases lacking significance, the trend was considered stable. For the statistical analysis of the spatial distribution of intoxications in general and intoxications by Senecio spp. in particular, a geographic coordinate system was used considering the 25 municipalities that are currently served by the LRD-UFPel (Fig.1). Maps were prepared with an interval of 21 years, referring to the incidence rates by municipality of intoxications in general and intoxications by Senecio spp. in particular, as this was the only intoxication type with a sufficient number of cases for this analysis. Municipalities that were emancipated after 1990 were not allocated in the comparative map of the first 21 years of the study. The maps were obtained based on data provided by the “Instituto Brasileiro de Geografia e Estatística” (Brazilian Institute of Geography and Statistics) (IBGE 2021) in shapefile format (.shp) and subsequently analyzed in QGIS 3.4.7 software. Fig.1. Map of the region served by the “Laboratório Regional de Diagnóstico” of the “Faculdade de Veterinária” at “Universidade Federal de Pelotas” (LRD-UFPel) included in the spatial analysis of the occurrence of poisonings in general and by Senecio spp. Results From January 1979 to December 2020, 7,406 bovine materials were received at the LRD-UFPel for diagnosis, with 3,753 cases received from 1979 to 1999 and 3,653 cases from 2000 to 2020, with an average of 176.3 cases being forwarded per year. Of these, 392 were diagnosed with poisoning, with 141 cases between 1979 and 1999 and 252 cases between 2000 and 2020. Out of 140 cases diagnosed in the first 21 years of activities of the LRD-UFPel, 113 (80.1%) were plant poisonings, 22 (15.6%) were fungi/mushroom poisonings and six (4.3%) were chemical poisonings (Table 1). From 2000 to 2020, of the 252 cases diagnosed (Table 2), 224 (88.88%) were caused by toxic plants, 10 (3.9%) by fungi/mushrooms, 12 (4.76%) by chemical substances and six (2.38%) by insects. The epidemiological data of each intoxication type, age interval between the youngest and oldest cattle affected, and time of occurrence are shown in Table 1 and 2. Table 1. Cases/outbreaks of poisoning by plants, fungi, and chemical substances diagnosed in cattle at the “Laboratório Regional de Diagnóstico” of the “Faculdade de Veterinária” at UFPel from 1979 to 1999 and the relationship between age, time of year, morbidity, mortality, and lethality Diagnostic 1979-1999 No. cases/ Outbreaks % Age Time of the year Morbidity % Mortality % Lethality % Plant poisoning 113 80.7 Senecio spp. 79 56.4 1.5-9 years All year 0.4-60 0.05-57.1 5.3-100 Solanum spp. 9 6.42 1-12 years May-Dec 7.1-13.3 0.03-0.04 50-100 Echium spp. 7 5.0 3 months-3 years Apr-Oct 0.5-39.1 0.5-29.7 76-100 Amaranthus spp. 5 3.57 3 months-4.5 years Apr-Jun 1.5-37.9 1.1-22.3 20-75 Baccharis coridifolia 4 2,85 3 months-2.5 years Mar-Sep 4.7-35.3 4.6-29.4 75-100 Xanthium spp. 3 2.14 6 months-3 years Agu-Sep 3.1-80.6 3.1-80.6 100 Cestrum parqui 2 1.42 6 months-3 years Jan-Aug 15-81.8 15-27.3 33.3-100 Ammi majus 1 0.7 Adults Mar 100 NI NI Prunus sellowii 1 0.7 Adults Jun NI NI NI Enterolobium contortisiliquum 1 0.7 3 years Jun 42.8 NI NI Sorghum 1 0.7 2-3 years Jun 13.3 10 75 Fungal/fungal toxins poisoning 22 15.7 Claviceps paspali 20 14.28 6 months-4 years Mar-May 0.3-33.3 0.1-6.2 10-100 Ramaria flavo-brunnescens 2 1.42 Adults May 10.6-26.6 1.2-6.7 11.8-25 Chemical substances poisoning 5 3.57 Urea 2 1.42 1 month-3 years Aug-Sep 0.5 0.5 100 Carbolineum 1 0.7 Adults Aug 2.7 0.7 25 Organophosphates 1 0.7 1 year Aug 90.5 90.5 100 Sodium chloride 1 0.7 7 years Dec 100 100 100 TOTAL 140 100 NI = not informed. Table 2. Cases/outbreaks of poisoning by plants, fungi, and chemical substances diagnosed in cattle at the “Laboratório Regional de Diagnóstico” of the “Faculdade de Veterinária” at UFPel from 2000 to 2020 and the relationship between age, time of year, morbidity, mortality, and lethality Diagnostic 2000-2020 No. cases/ Outbreaks % Age Time of the year Morbidity % Mortality % Lethality % Plant poisoning 224 88.88 Senecio spp. 202 80.15 3 months-10 years All year 0.1-100 0.1-100 16.6-100 Baccharis coridifolia 8 3.17 6 months-7 years Nov-Jun 0.4-100 0.4-100 56.52-100 Xanthium spp. 6 2.38 6 months-5 years Aug-Jan 1.16-57.14 1.16-57.14 100 Amaranthus spp. 5 1.98 1.5-8 years Jan-May 0.54-6.92 0.54-11.29 66.66-100 Prunus sellowii 1 0.39 4.5 years May 16.66 16.66 100 Solanum fastigiatum 1 0.39 4 years Jun 18 8 44.44 Dodonaea viscosa 1 0.39 4 years Apr 18.7 18.7 100 Fungal/fungal toxins poisoning 10 3.96 Claviceps paspali 3 1.19 6-9 months Apr-Jun 1.6-33.33 1.6-26.66 50-100 Aflatoxins 3 1.19 1-4anos Jun-Sep 2.94-8.5 0.5-11.76 5.88-100 Ramaria flavo-brunnescens 3 1.19 1.5 years May 2.58 34.83 100 Diplodia maydis 1 0.39 6 years Jun 25 25 100 Chemical substances poisoning 12 4.76 Organophosphates 7 2.77 7 days-5 years Oct-Jan 1.4-100 0.7-86.65 16-100 Ionophore antibiotics 3 1.19 2-3 years May-Sep 14-100 4.0-14 4-100 Abamectin 1 0.39 3-4 months May 6 6 100 Sodium chloride 1 0.39 3 years Jun 0.9 0.9 100 Insect poisoning 6 2.38 Perreyia flavipes 6 2.38 1-3 years Jul-Sep 0.8-3.70 0.8-38.70 100 TOTAL 252 100 In the temporal trend analysis, a significant linear trend was observed with values of p=0.003 and APC of 2.5 for the increase in the number of diagnoses of intoxications in general throughout the entire period of operation of the LRD, with no subtrends being observed (Fig.2). When the trends of intoxications, in general, were analyzed over periods of 21 years, no statistically significant values were found. When analyzing the temporal trend of poisoning by Senecio spp., there was an increase in the occurrence of this diagnosis, with different characteristics and turning points over time. From 1979 to 1999 and from 2000 to 2001, there was a significant increase in the number of poisonings by the Senecio spp., with p values <0.001 and APC of 9.1 and an annual increase of 9% in diagnoses during the first 21 years of operation of the LRD-UFPel. Over the other years (2002-2020), the APC values were not significant (Fig.3). Fig.2. Temporal analysis of the trend of occurrence of poisonings in general from 1979 to 2020 in the area served by the “Laboratório Regional de Diagnóstico” of the “Faculdade de Veterinária” at “Universidade Federal de Pelotas” (LRD-UFPel). Fig.3. Temporal analysis of the trend of occurrence of poisoning by Senecio spp. from 1979 to 2020 in the area served by the “Laboratório Regional de Diagnóstico” of the “Faculdade de Veterinária” at “Universidade Federal de Pelotas” (LRD-UFPel). Incidence rates of poisoning, in general, increased in the municipalities served by the LRD-UFPel between the compared periods (Fig.4). Cases of poisoning by Senecio spp. also showed a significant increase both in the number of municipalities affected and in the incidence rates of intoxication. Poisoning by Senecio spp. from 1979 to 1999 had an incidence in eight municipalities of 0.01 to 5.00 cases per total number of cattle in the municipality, and in the last two decades, the incidence was greater than 5.00 in nine municipalities. Fig.4. Statistical analysis of the spatial distribution of poisonings in general and by Senecio spp. From 1979 to 1999 and 2000 to 2020 in the area served by the “Laboratório Regional de Diagnóstico” of the “Faculdade de Veterinária” at “Universidade Federal de Pelotas” (LRD-UFPel). Discussion In the present study, diagnoses of intoxications increased from 141 cases/outbreaks in the first two decades of the study to 256 cases/outbreaks in the following two decades, with a significant increase in the annual percentage of intoxications (p=0.003 and APC 2.5). The number of bovine materials received from 1979 to 1999 was slightly higher than the number of materials/necropsies received from 2000 to 2020. This seems to suggest that the increase was not due to an increase in the number of bovine materials received but due to an actual increase in cases of intoxication. It was observed that the occurrence of intoxications from 1979 to 1999 represented 4% of all bovine materials/necropsies received in the laboratory and that there was a gradual increase from 2000 to 2020, reaching 7% in this animal species. The spread of toxic plants throughout the municipalities in the region and the improvement in the efficiency of the laboratory with the dissemination of information on its activities reaching a greater number of professionals may have influenced the gradual increase in diagnoses of poisonings in the area served by the LRD-UFPel over the years. Among intoxications, cases of poisoning by plants were the most frequent, with the number of cases of poisoning by Senecio spp. being the highest, which was also observed in beef cattle in other regions of Rio Grande do Sul (Molossi et al. 2021). However, in the first 21 years of the study, the increase was significant (p<0.001), and in the last 21 years, there was no consistency in the annual number of diagnoses. The significant increase in the first 21 years can be explained by the beginning of the activities of the LRD-UFPel and the disclosure of information to farmers and veterinarians on the different intoxications that occurred in the region, and in particular about poisoning by Senecio spp. This intoxication has been studied and recognized as a cause of great economic losses in livestock for more than four decades in Rio Grande do Sul (Méndez et al. 1987, Karam et al. 2004, Rissi et al. 2007, Lucena et al. 2010, Panziera et al. 2018). It should be noted that in the second period of the study, from 2000 to 2020, peaks of increase in the diagnosis of this intoxication were observed. One of them, from 2004 to 2008, probably occurred due to the severe drought in the region (Grecco et al. 2010), and the other, from 2011, possibly occurred due to the great spread of Senecio madagascariensis, a species with great dispersal capacity and which does not found in the region in previous years, having caused several outbreaks associated with mortality in the southern region of the state in 2013 (Stigger et al. 2014). It is noteworthy that in the first period of the study, poisoning by Senecio spp. was observed in cattle older than two years of age, probably due to its chronic character (Méndez et al. 1987, Driemeier et al. 1991, Barros et al. 1992), but in the last two decades, outbreaks in young cattle with less than 1.5 years of age occurred in the region, suggesting greater availability of the plant or increased concentration of pyrrolizidine alkaloids (Grecco et al. 2010). Echium plantagineum, a toxic plant with the same active principle as Senecio spp., caused intoxication outbreaks only in the first 21 years of the study, representing 5% of intoxications. This is a plant whose toxicity is highly variable and it grows only in pastures in the first year of implantation (Méndez et al. 1985). The absence of outbreaks in the last 20 years reinforces the concept that there is variation in plant toxicity, since in some areas where E. plantagineum was found, there were no cases of intoxication. Additionally, the use of certified seeds has been adopted by many farmers to improve the quality of pastures, helping to prevent E. plantagineum from infesting pastures or crop fields by not planting seed mixtures that may contain this invasive species (Stigger et al. 2014). Intoxication by Solanum fastigiatum occurred more frequently from 1979 to 1999, being diagnosed in 6.4% of cases, compared to the last 21 years in which it was observed only once (0.4%). The low morbidity and mortality associated with the chronic character of this intoxication may explain the considerable decrease in the number of cases, since after the identification of this plant as toxic in the first decade of this study (Riet-Correa et al. 1983), when the first clinical signs are observed, producers typically remove the animals from the areas where the plant occurs or send them to slaughter, and thus do not send materials to the laboratory. Outbreaks of Baccharis coridifolia poisoning were observed in 2.8% and 3.1% of cases in the two study periods, respectively. Intoxication by this plant has been known since the time of the colonization of the Americas (Cobo 1964), and management measures to prevent its occurrence have been known and publicized for many decades. Some of these measures have been tested and shown to be effective in preventing outbreaks (Almeida et al. 2013). This is probably one of the reasons why the percentage of occurrence has remained stable. Some cases still occur because the control measures are not applied properly or because they are not effective, as when the plant is burned, which causes the animals to inhale the smoke, a measure that continues to be used and, in general, does not prevent cattle from consuming the plant (Almeida et al. 2013). Poisoning by Xanthium spp. occurred in 2.1% of cases of poisoning from 1979 to 1999 and in 2.3% of cases from 2000 to 2020. The percentage of cases was similar in both study periods. It is noteworthy that in all outbreaks, poisoning occurred by ingesting the cotyledons of the budding plant after heavy rains from late winter to early summer. The southern region of the Rio Grande do Sul State is characterized by flat and swampy fields, and outbreaks occurred on properties located on the banks of rivers or streams (Riet-Correa et al. 1983, 1984, Alberti et al. 2020), where the ideal environmental conditions for plant growth occur (Alberti et al. 2020). Poisoning by Xanthium spp. through ingestion of food contaminated by seeds is unlikely to occur in the region because cattle are commonly raised in pastures and are not fed crop residues (Driemeier et al. 1999). This was also observed in cattle confined in the region where the only poisoning diagnosed was by Senecio spp., having no relation to the ingestion of contaminated food (Estima-Silva et al. 2020). Outbreaks of poisoning by Amaranthus spp. represented 3.5% of diagnoses of toxic diseases in the first 21 years and 2% in the subsequent 21 years, with no significant variation in the percentage of diagnoses in the two studied periods. Despite not being an important toxic plant from the point of view of the number of outbreaks, it is associated with high lethality that can reach 100% (Stigger et al. 2013). This plant can grow on fertilized land, and outbreaks can occur when cattle are grazed on crop stubbles that have not been completely harvested, especially when there is drought and lack of fodder in summer and autumn (Ferreira et al. 1991, Lemos et al. 1993, Torres et al. 1997). From 2000 to 2020, two outbreaks were described on a farm where Amaranthus spp. invaded pastures of Brachiaria sp. and, therefore, there was no shortage of forage, indicating that this intoxication can also occur in situations of adequate food quantity but with a large infestation by the plant that is palatable for cattle (Riet-Correa et al. 1983, Ferreira et al. 1991, Stigger et al. 2013). Among the cases of mycotoxicosis diagnosed in Rio Grande do Sul, poisoning by Claviceps paspali was the most frequent, accounting for 14.5% of the diagnoses of poisoning made in the first 21 years of the study and only 1.2% since the 2000s. This sharp decrease in the number of diagnoses probably occurred for two reasons: first, intoxication became known to veterinarians and farmers after diagnosis in the first decades of operation of the LRD-UFPel; second, it is a disease with pronounced neurological clinical signs but with low mortality and high animal recovery (Rissi et al. 2007, Riet-Correa et al. 2013). Cases may not have been reported for this last reason. It is likely that a greater number of outbreaks have occurred in the last 20 years and they were often reported but not registered in the LRD-UFPel due to the lack of material for necropsy and/or histopathology. The low number of mycetism diagnoses involving Ramaria flavo-brunnescens in the two periods (1.4% and 1.2%) suggests that knowledge about the disease may have, to a large extent, prevented outbreaks through the management of areas of eucalyptus forests by fencing or by removing animals from these areas during the autumn months (Alves et al. 2014, Scheid et al. 2022). Additionally, the large annual variation in the occurrence of the mushroom and its toxicity is another factor in the reduction of intoxication outbreaks. In southern Rio Grande do Sul, in 2020 and 2021, a small occurrence of the mushroom was reported in eucalyptus forests in the municipalities of Pelotas, Capão do Leão, and Jaguarão, where the disease has been observed (Scheid et al. 2022). This is probably a consequence of the drought observed in the period and is in accordance with what was reported in several studies about the climatic influences on the occurrence of the mushroom and its toxicity (Sallis et al. 2004, Riet-Correa et al. 2004). With regard to poisoning by chemical substances, it was observed that there was no marked change in the percentage of occurrence when comparing the first two decades with the final two decades of the study. These poisonings accounted for 4.3% and 6.2% of cases from 1979 to 1999 and from 2000 to 2020, respectively. In this group, organophosphate poisoning stands out. Intoxication by organophosphates has increased considerably in the last two decades, accounting for 0.7% to 3.5% of diagnoses of toxic diseases in cattle. This may be related to the greater availability of this active principle for use in animals, since these poisonings occur fundamentally due to human error, such as errors in dosage or route of application (Grecco et al. 2009, Santos et al. 2014). It should also be considered that the nine outbreaks that occurred in the summer of 2013-2014 were influenced by the atypical heat that occurred in relation to normal averages for the region and that excessive heat exacerbates the absorption of the active principle (Santos et al. 2014). Poisoning by Perreyia flavipes was observed only in the second period of the study, representing 2.3% of cases of toxic diseases. This intoxication was diagnosed in Uruguay in the 1990s (Dutra et al. 1997), and cases in southern Rio Grande do Sul were observed in 2006 and 2011 (Soares et al. 2008). This is an intoxication that depends on suitable climatic conditions for its occurrence (Raymundo et al. 2009), and for this reason, the number of outbreaks is variable and does not occur frequently. Other plant poisonings and mycotoxicoses occurred sporadically in the two periods of study, and their importance in the context of toxic diseases in cattle was relatively small. Some plants, such as Cestrum sp. and Dodonaea viscosa, are more important from the point of view of differential diagnosis, reinforcing the need for their identification, since they cause toxic diseases similar to those caused by Xanthium spp. and P. flavipes. According to the spatial analysis, there was a significant increase in the incidence of poisoning in general in eight municipalities in the region served by the laboratory compared to the incidence in the same region in the first 21 years of the study. This demonstrates that there has been a real increase in cases of toxic diseases in the region, which may also be associated with the dissemination of the activities of the laboratory diagnostic service. It is noteworthy that poisoning by Senecio spp. from 1979 to 1999 had an incidence in eight municipalities from 0.01 to 5.00, and in the last two decades, its incidence was greater than 5.00 in nine municipalities, demonstrating the increase in cases of this intoxication in the region. Conclusions The results of this study allowed us to conclude that there was a significant linear temporal trend in the number of diagnoses of poisonings in the southern region of Rio Grande do Sul. The percentage of outbreaks of poisoning by Solanum fastigiatum, Echium plantagineum, and Claviceps paspali decreased in the last 21 years of the study. The poisonings by Ramaria flavo-brunnescens and Baccharis coridifolia occurred during the 42 years without significant variation. In the first 21 years of the study, there was a temporal trend toward an increase of 9% per year in cases of poisoning by Senecio spp. There was an increase in the absolute number of cases of poisoning by Senecio spp. in the comparison between the first 21 years of the study and the subsequent 21 years. Organophosphate poisoning has increased considerably over the last two decades and is fundamentally related to errors in dosage and route of application in both periods. The poisonings will remain an important cause of death in cattle in the region despite the wide disclosure of information on their occurrence. Acknowledgments The authors are grateful to “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq), and “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior” (CAPES) (finance code 001) for scientific, financial support and student’s scholarship. References Alberti T.S., Zamboni R., Scheid H.V., Venancio F.R., Brunner C.B., Raffi M.B., Schild A.L. & Sallis E.S.V. 2020. Spontaneous poisoning by sprouts of Xanthium spp. in cattle in Southern Rio Grande do Sul. Acta Scient. Vet. 48(Supl.1):507. <https://dx.doi.org/10.22456/1679-9216.99161> Alberti T.S. Zamboni R. Scheid H.V. Venancio F.R. Brunner C.B. Raffi M.B. Schild A.L. Sallis E.S.V. 2020 Spontaneous poisoning by sprouts of Xanthium spp. in cattle in Southern Rio Grande do Sul Acta Scient. Vet. 48 Supl.1 507 https://dx.doi.org/10.22456/1679-9216.99161 Almeida M.B., Schild A.L., Pfister J., Pimentel M., Forster K.M. & Riet-Correa F. 2013. Methods of inducing conditioned food aversion to Baccharis coridifolia (mio-mio) in cattle. Ciência Rural 43(10):1866-1871. <https://dx.doi.org/10.1590/S0103-84782013001000022> Almeida M.B. Schild A.L. Pfister J. Pimentel M. Forster K.M. Riet-Correa F. 2013 Methods of inducing conditioned food aversion to Baccharis coridifolia (mio-mio) in cattle Ciência Rural 43 10 1866 1871 https://dx.doi.org/10.1590/S0103-84782013001000022 Alves D.M., Marcolongo-Pereira C., Tavares L.A., Molarinho K.R., Raffi M.B., Schild A.L. & Sallis E.S.V. 2014. Criação de bovinos em sistema silvipastoril com eucalipto: um risco para intoxicação por Ramaria flavo-brunnescens. Pesq. Vet. Bras. 34(7):659-662. <https://dx.doi.org/10.1590/S0100-736X2014000700009> Alves D.M. Marcolongo-Pereira C. Tavares L.A. Molarinho K.R. Raffi M.B. Schild A.L. Sallis E.S.V. 2014 Criação de bovinos em sistema silvipastoril com eucalipto: um risco para intoxicação por Ramaria flavo-brunnescens Pesq. Vet. Bras. 34 7 659 662 https://dx.doi.org/10.1590/S0100-736X2014000700009 Barros S.S. 1958. Da possível incidência da toxicose pelo selênio em bovinos no Rio Grande do Sul. Dipan, Porto Alegre, 11:10-13. Barros S.S. 1958 Da possível incidência da toxicose pelo selênio em bovinos no Rio Grande do Sul Dipan Porto Alegre 11 10 13 Barros C.S., Driemeier D., Pilati C., Barros S.S. & Castilhos L.M. 1992. Senecio spp. poisoning in cattle in southern Brazil. Vet. Human Toxicol. 34(3):241-246. <PMid:1609498> Barros C.S. Driemeier D. Pilati C. Barros S.S. Castilhos L.M. 1992 Senecio spp. poisoning in cattle in southern Brazil Vet. Human Toxicol. 34 3 241 246 1609498 Cobo B. 1964. Historia del Nuevo Mundo. Biblioteca de Autores Españoles, Madrid, 568p. Cobo B. 1964 Historia del Nuevo Mundo Biblioteca de Autores Españoles Madrid 568p Driemeier D., Barros C.S.L. & Pilati C. 1991. Seneciose em bovinos. Hora Vet. 10(59):23-30. Driemeier D. Barros C.S.L. Pilati C. 1991 Seneciose em bovinos Hora Vet. 10 59 23 30 Driemeier D., Irigoyen L.F., Loretti A.P., Colodel E.M. & Barros C.S.L. 1999. Intoxicação espontânea pelos frutos de Xanthium cavanillesii (Asteraceae) em bovinos no Rio Grande do Sul. Pesq. Vet. Bras. 19(1):12-18. <https://dx.doi.org/10.1590/S0100-736X1999000100003> Driemeier D. Irigoyen L.F. Loretti A.P. Colodel E.M. Barros C.S.L. 1999 Intoxicação espontânea pelos frutos de Xanthium cavanillesii (Asteraceae) em bovinos no Rio Grande do Sul Pesq. Vet. Bras. 19 1 12 18 https://dx.doi.org/10.1590/S0100-736X1999000100003 Dutra F., Riet-Correa F., Mendez M.C. & Paiva N. 1997. Poisoning of cattle and sheep in Uruguay by sawfly (Perreyia flavipes) larvae. Vet. Hum. Toxicol. 39(5):281-286. <PMid:9311084> Dutra F. Riet-Correa F. Mendez M.C. Paiva N. 1997 Poisoning of cattle and sheep in Uruguay by sawfly (Perreyia flavipes) larvae Vet. Hum. Toxicol. 39 5 281 286 9311084 Estima-Silva P., Oliveira P.A., Bruhn F.R.P., Scheid H.V., Marques L.S., Ribeiro L.S. & Schild A.L. 2020. Causes of death of beef cattle raised in feedlots. Pesq. Vet. Bras. 40(5):333-339. <https://dx.doi.org/10.1590/1678-5150-PVB-6539> Estima-Silva P. Oliveira P.A. Bruhn F.R.P. Scheid H.V. Marques L.S. Ribeiro L.S. Schild A.L. 2020 Causes of death of beef cattle raised in feedlots Pesq. Vet. Bras. 40 5 333 339 https://dx.doi.org/10.1590/1678-5150-PVB-6539 Ferreira J.L.M., Riet-Correa F., Schild A.L. & Méndez M.C. 1991. Intoxicação por Amaranthus spp. (Amaranthaceae) em bovinos no Rio Grande do Sul. Pesq. Vet. Bras. 11(3/4):49-54. Ferreira J.L.M. Riet-Correa F. Schild A.L. Méndez M.C. 1991 Intoxicação por Amaranthus spp. (Amaranthaceae) em bovinos no Rio Grande do Sul Pesq. Vet. Bras. 11 3/4 49 54 Grecco F.B., Schild A.L., Soares M.P., Pereira C.M., Estima-Silva P. & Sallis E.S.V. 2010. Aspectos epidemiológicos e padrões de lesões hepáticas em 35 surtos de intoxicação por Senecio spp. em bovinos no sul do Rio Grande do Sul. Pesq. Vet. Bras. 30(5):389-397. <https://dx.doi.org/10.1590/S0100-736X2010000500003> Grecco F.B. Schild A.L. Soares M.P. Pereira C.M. Estima-Silva P. Sallis E.S.V. 2010 Aspectos epidemiológicos e padrões de lesões hepáticas em 35 surtos de intoxicação por Senecio spp. em bovinos no sul do Rio Grande do Sul Pesq. Vet. Bras. 30 5 389 397 https://dx.doi.org/10.1590/S0100-736X2010000500003 Grecco F.B., Schild A.L., Soares M.P., Rafii M.B., Sallis E.S.V. & Damé M.C. 2009. Intoxicação por organofosforados em búfalos (Bubalus bubalis) no Rio Grande do Sul. Pesq. Vet. Bras. 29(3):211-214. <https://dx.doi.org/10.1590/S0100-736X2009000300004> Grecco F.B. Schild A.L. Soares M.P. Rafii M.B. Sallis E.S.V. Damé M.C. 2009 Intoxicação por organofosforados em búfalos (Bubalus bubalis) no Rio Grande do Sul Pesq. Vet. Bras. 29 3 211 214 https://dx.doi.org/10.1590/S0100-736X2009000300004 IBGE 2021. Produção da Pecuária Municipal. 2020. Available at <Available at <Available at http://www.ibge.gov.br/home/estatistica/economia/ppm/2021/default.shtm > Accessed on Out. 28, 2021. IBGE 2021 Produção da Pecuária Municipal. 2020 Available at http://www.ibge.gov.br/home/estatistica/economia/ppm/2021/default.shtm Out. 28, 2021 Karam F.S.C., Soares M.P., Haraguchi M., Riet-Correa F., Méndez M. del C. & Jarenkow J.A. 2004. Aspectos epidemiológicos da seneciose na região sul do Rio Grande do Sul, Brasil. Pesq. Vet. Bras. 24(4):191-198. <https://dx.doi.org/10.1590/S0100-736X2004000400004> Karam F.S.C. Soares M.P. Haraguchi M. Riet-Correa F. Méndez M. del C. Jarenkow J.A. 2004 Aspectos epidemiológicos da seneciose na região sul do Rio Grande do Sul, Brasil Pesq. Vet. Bras. 24 4 191 198 https://dx.doi.org/10.1590/S0100-736X2004000400004 Lemos R.A., Barros C.S.L., Salles M.S., Barros S.S. & Peixoto P.V. 1993. Intoxicação espontânea por Amaranthus spinosus (Amaranthaceae) em bovinos. Pesq. Vet. Bras. 13(1/2):25-34. Lemos R.A. Barros C.S.L. Salles M.S. Barros S.S. Peixoto P.V. 1993 Intoxicação espontânea por Amaranthus spinosus (Amaranthaceae) em bovinos Pesq. Vet. Bras. 13 1/2 25 34 Lucena R.B., Rissi D.R., Maia L.A., Flores M.M., Dantas A.F.M., Nobre V.M.T., Riet-Correa F. & Barros C.S.L. 2010. Intoxicação por alcaloides pirrolizidínicos em ruminantes e equinos no Brasil. Pesq. Vet. Bras. 30(5):447-452. <https://dx.doi.org/10.1590/S0100-736X2010000500013> Lucena R.B. Rissi D.R. Maia L.A. Flores M.M. Dantas A.F.M. Nobre V.M.T. Riet-Correa F. Barros C.S.L. 2010 Intoxicação por alcaloides pirrolizidínicos em ruminantes e equinos no Brasil Pesq. Vet. Bras. 30 5 447 452 https://dx.doi.org/10.1590/S0100-736X2010000500013 Méndez M.A., Riet-Correa F., Schild A.L. & Garcia J.T.C. 1985. Poisoning by Echium plantagineum (Boraginaceae) in cattle in southern Brazil. Pesq. Vet. Bras. 5(2):57-64 Méndez M.A. Riet-Correa F. Schild A.L. Garcia J.T.C. 1985 Poisoning by Echium plantagineum (Boraginaceae) in cattle in southern Brazil Pesq. Vet. Bras. 5 2 57 64 Méndez M.D.C., Riet-Correa F. & Schild A.L. 1987. Intoxicação por Senecio spp. (Compositae) em bovinos no Rio Grande do Sul. Pesq. Vet. Bras. 7(2):51-56. Méndez M.D.C. Riet-Correa F. Schild A.L. 1987 Intoxicação por Senecio spp. (Compositae) em bovinos no Rio Grande do Sul Pesq. Vet. Bras. 7 2 51 56 Molossi F.A., Cecco B.S., Pohl C.B., Borges R.B., Sonne L., Pavarini S.P. & Driemeier D. 2021. Causes of death in beef cattle in southern Brazil. J. Vet. Diagn. Invest. 33(4):677-683. <https://dx.doi.org/10.1177/10406387211007952> <PMid:33834923> Molossi F.A. Cecco B.S. Pohl C.B. Borges R.B. Sonne L. Pavarini S.P. Driemeier D. 2021 Causes of death in beef cattle in southern Brazil J. Vet. Diagn. Invest. 33 4 677 683 https://dx.doi.org/10.1177/10406387211007952 33834923 Monquero P.A. 2005. Plantas transgênicas resistentes aos herbicidas: situação e perspectivas. Bragantia 64(4):517-531. <https://dx.doi.org/10.1590/S0006-87052005000400002> Monquero P.A. 2005 Plantas transgênicas resistentes aos herbicidas: situação e perspectivas Bragantia 64 4 517 531 https://dx.doi.org/10.1590/S0006-87052005000400002 Panziera W., Pavarini S.P., Sonne L., Barros C.S.L. & Driemeier D. 2018. Poisoning of cattle by Senecio spp. in Brazil: a review. Pesq. Vet. Bras. 38(8):1459-1470. <https://dx.doi.org/10.1590/1678-5150-PVB-5795> Panziera W. Pavarini S.P. Sonne L. Barros C.S.L. Driemeier D. 2018 Poisoning of cattle by Senecio spp. in Brazil: a review Pesq. Vet. Bras. 38 8 1459 1470 https://dx.doi.org/10.1590/1678-5150-PVB-5795 Pessoa C.R.M., Medeiros R.M.T. & Riet-Correa F. 2013. Importância econômica, epidemiologia e controle das intoxicações por plantasno Brasil. Pesq. Vet. Bras. 33(6):752-758. <https://dx.doi.org/10.1590/S0100-736X2013000600011> Pessoa C.R.M. Medeiros R.M.T. Riet-Correa F. 2013 Importância econômica, epidemiologia e controle das intoxicações por plantasno Brasil Pesq. Vet. Bras. 33 6 752 758 https://dx.doi.org/10.1590/S0100-736X2013000600011 Raymundo D.L., Bezerra Junior P.S., Bandarra P.M., Santos A.S., Sonne L., Pavarini S.P., Correa A.M.R., Dias M.M. & Driemeier D. 2009. Intoxicação espontânea pelas larvas de Perreyia lavipes em bovinos no Estado de Santa Catarina, Brasil. Ciência Rural 39(1):163-166. <https://dx.doi.org/10.1590/S0103-84782009000100025> Raymundo D.L. Bezerra P.S. Junior Bandarra P.M. Santos A.S. Sonne L. Pavarini S.P. Correa A.M.R. Dias M.M. Driemeier D. 2009 Intoxicação espontânea pelas larvas de Perreyia lavipes em bovinos no Estado de Santa Catarina, Brasil Ciência Rural 39 1 163 166 https://dx.doi.org/10.1590/S0103-84782009000100025 Riet-Correa F. & Medeiros R.M.T. 2001. Intoxicações por plantas em ruminantes no Brasil e no Uruguai: importância econômica, controle e riscos para a saúde pública. Pesq. Vet. Bras. 21(1):38-42. <https://dx.doi.org/10.1590/S0100-736X2001000100008> Riet-Correa F. Medeiros R.M.T. 2001 Intoxicações por plantas em ruminantes no Brasil e no Uruguai: importância econômica, controle e riscos para a saúde pública Pesq. Vet. Bras. 21 1 38 42 https://dx.doi.org/10.1590/S0100-736X2001000100008 Riet-Correa F., Barros C.S.L. & Schild A.L. 2004. Intoxication by Ramaria flavo brunnescens in domestic animals, p.488-493. In: Acamovic T., Stewart C.S. & Pennycott T.W. (Eds), Poisonous Plants and Related Toxins. CAB International, Wallingford, UK. Riet-Correa F. Barros C.S.L. Schild A.L. 2004 Intoxication by Ramaria flavo brunnescens in domestic animals 488 493 Acamovic T. Stewart C.S. Pennycott T.W. Poisonous Plants and Related Toxins CAB International Wallingford, UK Riet-Correa F., Méndez M.C., Schild A.L., Meireles M.C.A. & Scarsi R.M. 1984. Laboratório Regional de Diagnóstico - Doenças Diagnosticadas no Ano 1983. Ed. Universidade Pelotas, Pelotas, p.32-33. (Documento 5). Riet-Correa F. Méndez M.C. Schild A.L. Meireles M.C.A. Scarsi R.M. 1984 Laboratório Regional de Diagnóstico - Doenças Diagnosticadas no Ano 1983 Ed. Universidade Pelotas Pelotas 32 33 Documento 5 Riet-Correa F., Rivero R., Odriozola E., Adrien M.L., Medeiros R.M.T. & Schild A.L. 2013Mycotoxicoses of ruminants and horses. J. Vet. Diagn. Invest. 25(6):692-708. <https://dx.doi.org/10.1177/1040638713504572> <PMid:24091682> Riet-Correa F. Rivero R. Odriozola E. Adrien M.L. Medeiros R.M.T. Schild A.L. 2013 Mycotoxicoses of ruminants and horses J. Vet. Diagn. Invest. 25 6 692 708 https://dx.doi.org/10.1177/1040638713504572 24091682 Riet-Correa F., Schild A.L., Méndez M.C., Oliveira J.A., Gil-Turnes C. & Gonçalves A. 1983. Atividades do Laboratório Regional de Diagnóstico e Doenças da Área de Influęncia no Período 1978-1982. Ed. Universidade Pelotas, Pelotas, p.87-88. (Documento 4). Riet-Correa F. Schild A.L. Méndez M.C. Oliveira J.A. Gil-Turnes C. Gonçalves A. 1983 Atividades do Laboratório Regional de Diagnóstico e Doenças da Área de Influęncia no Período 1978-1982 Ed. Universidade Pelotas Pelotas 87 88 Documento 4 Rissi D.R., Rech R.R., Pierezan F., Gabriel A.L., Trost M.E., Brum J.S., Kommers G.D. & Barros C.S.L. 2007. Intoxicações por plantas e micotoxinas associadas a plantas em bovinos no Rio Grande do Sul: 461 casos. Pesq. Vet. Bras. 27(7):261-268. <https://dx.doi.org/10.1590/S0100-736X2007000700002> Rissi D.R. Rech R.R. Pierezan F. Gabriel A.L. Trost M.E. Brum J.S. Kommers G.D. Barros C.S.L. 2007 Intoxicações por plantas e micotoxinas associadas a plantas em bovinos no Rio Grande do Sul: 461 casos Pesq. Vet. Bras. 27 7 261 268 https://dx.doi.org/10.1590/S0100-736X2007000700002 Sallis E.S.V., Raffi M.B. & Riet-Correa F. 2004 Intoxicação experimental em ovinos com Ramaria flavo- brunnescens congelada ou dessecada. Pesq. Vet. Bras. 24(2):107-110. <https://dx.doi.org/10.1590/S0100-736X2004000200010> Sallis E.S.V. Raffi M.B. Riet-Correa F. 2004 Intoxicação experimental em ovinos com Ramaria flavo- brunnescens congelada ou dessecada Pesq. Vet. Bras. 24 2 107 110 https://dx.doi.org/10.1590/S0100-736X2004000200010 Santos B.L., Marcolongo-Pereira C., Stigger A.L., Coelho A.C.B., Soares M.P., Sallis E.S.V., Barreto F. & Schild A.L. 2014. Uso inadequado de organofosforados: uma prática de risco para bovinos no Sul do Rio Grande do Sul. Pesq. Vet. Bras. 34(7):655-658. <https://dx.doi.org/10.1590/S0100-736X2014000700008> Santos B.L. Marcolongo-Pereira C. Stigger A.L. Coelho A.C.B. Soares M.P. Sallis E.S.V. Barreto F. Schild A.L. 2014 Uso inadequado de organofosforados: uma prática de risco para bovinos no Sul do Rio Grande do Sul Pesq. Vet. Bras. 34 7 655 658 https://dx.doi.org/10.1590/S0100-736X2014000700008 Scheid H.V., Sallis E.S.V., Riet-Correa F. & Schild A.L. 2022. Ramaria flavo-brunnescens mushroom poisoning in South America: a comprehensive review. Toxicon, 205:91-98. <https://dx.doi.org/10.1016/j.toxicon.2021.12.001> <PMid:34883101> Scheid H.V. Sallis E.S.V. Riet-Correa F. Schild A.L. 2022 Ramaria flavo-brunnescens mushroom poisoning in South America: a comprehensive review Toxicon 205 91 98 https://dx.doi.org/10.1016/j.toxicon.2021.12.001 34883101 Soares M.P., Quevedo P.S. & Schild A.L. 2008. Intoxicação por larvas de Perreyia flavipes em bovinos na região sul do Rio Grande do Sul. Pesq. Vet. Bras. 28(3):169-173. <https://dx.doi.org/10.1590/S0100-736X2008000300007> Soares M.P. Quevedo P.S. Schild A.L. 2008 Intoxicação por larvas de Perreyia flavipes em bovinos na região sul do Rio Grande do Sul Pesq. Vet. Bras. 28 3 169 173 https://dx.doi.org/10.1590/S0100-736X2008000300007 Stigger A.L., Estima-Silva P., Fiss L., Coelho A.C.B., Santos B.L., Gardner D.R., Marcolongo-Pereira C. & Schild A.L. 2014. Senecio madagascariensis Poir. (Asteraceae): uma nova causa de seneciose em bovinos no Sul do Rio Grande do Sul. Pesq. Vet. Bras. 34(9):851-855. <https://dx.doi.org/10.1590/S0100-736X2014000900008> Stigger A.L. Estima-Silva P. Fiss L. Coelho A.C.B. Santos B.L. Gardner D.R. Marcolongo-Pereira C. Schild A.L. 2014 Senecio madagascariensis Poir. (Asteraceae): uma nova causa de seneciose em bovinos no Sul do Rio Grande do Sul Pesq. Vet. Bras. 34 9 851 855 https://dx.doi.org/10.1590/S0100-736X2014000900008 Stigger A.L., Marcolongo-Pereira C., Adrien M.L., Santos B.L., Fiss L., Vargas Jr. S.F., Grecco F.B. & Schild A.L. 2013. Intoxicação espontânea por Amaranthus hybridus (Amaranthaceae) em bovinos no sul do Rio Grande do Sul. Pesq. Vet. Bras. 33(8):1004-1008. <https://dx.doi.org/10.1590/S0100-736X2013000800010> Stigger A.L. Marcolongo-Pereira C. Adrien M.L. Santos B.L. Fiss L. Jr. S.F. Vargas Grecco F.B. Schild A.L. 2013 Intoxicação espontânea por Amaranthus hybridus (Amaranthaceae) em bovinos no sul do Rio Grande do Sul Pesq. Vet. Bras. 33 8 1004 1008 https://dx.doi.org/10.1590/S0100-736X2013000800010 Tokarnia C.H., Brito M.F., Barbosa J.D., Peixoto P.V. & Döbereiner J. 2012. Plantas Tóxicas do Brasil. 2ª ed. Helianthus, Rio de Janeiro. 566p. Tokarnia C.H. Brito M.F. Barbosa J.D. Peixoto P.V. Döbereiner J. 2012 Plantas Tóxicas do Brasil 2ª ed. Helianthus Rio de Janeiro 566p Tokarnia C.H., Döbereiner J. & Peixoto P.V. 2000. Plantas Tóxicas do Brasil. Helianthus, Rio de Janeiro. 310p. Tokarnia C.H. Döbereiner J. Peixoto P.V. 2000 Plantas Tóxicas do Brasil Helianthus Rio de Janeiro 310p Tokarnia C.H., Döbereiner J. & Silva M.F. 1979. Plantas Tóxicas da Amazônia a Bovinos e Outros Herbívoros. Instituto Nacional de Pesquisas da Amazônia, Manaus. 95p. Tokarnia C.H. Döbereiner J. Silva M.F. 1979 Plantas Tóxicas da Amazônia a Bovinos e Outros Herbívoros Instituto Nacional de Pesquisas da Amazônia Manaus 95p Torres M.B., Kommers G.D., Dantas A.F.M. & Barros C.S.L. 1997. Redroot pigweed (Amaranthus retroflexus) poisoning of cattle in Southern Brazil. Vet. Hum. Toxicol. 39(2):94-96. <PMid:9080636> Torres M.B. Kommers G.D. Dantas A.F.M. Barros C.S.L. 1997 Redroot pigweed (Amaranthus retroflexus) poisoning of cattle in Southern Brazil Vet. Hum. Toxicol. 39 2 94 96 9080636