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Molecular characterization of part of the gag gene of caprine arthritis-encephalitis virus isolated from naturally infected goats from Rio Grande do Sul, Brazil

The gag gene of 5 CAEV samples, isolated from naturally infected goats from Rio Grande do Sul, Brazil, were analised by PCR and restriction endonuclease (DdeI, HaeIII e NdeI) digestion. Fragments of about 600 bp were amplified by PCR and submitted to enzymatic digestion. The patterns observed were compared with the corresponding gag sequences from 6 small ruminant lentiviruses. The results obtained allowed the separation of 3 distinct groups. The restriction fragment profiles observed were different from those previously described.

Caprine arthritis-encephalitis virus; lentivirus; PCR; RFLP


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