Função hepática de éguas submetidas à inoculação de antígeno botrópico para produção de soro antiofídico

Acidentes ofídicos têm sido um desafio médico e veterinário. O único tratamento disponível e preconizado pela Organização Mundial da Saúde e pelo Ministério Saúde é a soroterapia. O presente estudo avaliou a função hepática dos equinos submetidos ao protocolo de imunização empregado atualmente pelo Instituto Vital Brazil para produção do soro antibotrópico. Foram avaliados cinco equinos com inoculação de veneno Botrópico durante o período de três ciclos de imunizações. Amostras de sangue foram coletadas para realização de exame bioquímico e avaliação hepática por conta das lesões causadas no fígado. Os dados bioquímicos que apresentaram diferenças significativas, em todos os animais, comparados ao tempo basal, foram AST, ALT, GGT, FA, GLDH e proteína sérica. Creatinina não apresentou alteração ao longo do experimento. Os resultados obtidos sugerem que ao longo dos três ciclos de imunização podem ocorrer alterações hepáticas. Há uma grande necessidade de novos estudos com objetivo de ampliar o conhecimento acerca dos protocolos de inoculação de veneno nos equinos soroprodutores e minimizar os efeitos adversos da imunização.

TERMOS DE INDEXAÇÃO:
Equinos; soro antiofídico; fígado; GLDH

Autoria

Mariana N. Del Castillo ^**Corresponding author: mariana_nahum@hotmail.com

Departamento de Pós-Graduação em Clínica e Reprodução Animal, Universidade Federal Fluminense (UFF), Rua Vital Brazil Filho 64, Santa Rosa, Niterói, RJ 24230340, Brazil.Universidade Federal FluminenseBrazilNiterói, RJ, BrazilDepartamento de Pós-Graduação em Clínica e Reprodução Animal, Universidade Federal Fluminense (UFF), Rua Vital Brazil Filho 64, Santa Rosa, Niterói, RJ 24230340, Brazil.

https://orcid.org/0000-0001-7632-531X

Camila S.C. Ferreira

https://orcid.org/0000-0002-5902-0474

Mario F.A. Balaro

https://orcid.org/0000-0002-8198-7964

Patrícia Castanheira

Departamento de Antígeno e Cultivo Celular, Instituto Vital Brazil (IVB), Rua Maestro José Botelho 64, Vital Brasil, Niterói, RJ 24230-410, Brazil.Instituto Vital BrazilBrazilNiterói, RJ, BrazilDepartamento de Antígeno e Cultivo Celular, Instituto Vital Brazil (IVB), Rua Maestro José Botelho 64, Vital Brasil, Niterói, RJ 24230-410, Brazil.

https://orcid.org/0009-0005-7311-6199

Nadia R.P. Almosny

https://orcid.org/0000-0001-7043-0717

Aline E. Pinna

https://orcid.org/0000-0002-8954-2054

*Corresponding author: mariana_nahum@hotmail.com

Conflict of interest statement.- The authors declare that there is no conflict of interest.

SCIMAGO INSTITUTIONS RANKINGS

Figuras | Tabelas

Figuras (5)
Tabelas (1)

Thumbnail

Fig.1.
Three-cycle immunization protocol for Bothrops venom used by the “Instituto Vital Brazil” in equines.

Thumbnail

Fig.2.
Evaluation of total protein and serum albumin in equines throughout the experimental period. Total proteins demonstrated an effect of time, with an increase starting at S5 and remaining stable until the end of the study at S20. Albumin, on the other hand, demonstrated that there was no effect of time between baseline (S0) and the last week of collection (S20).

Thumbnail

Fig.3.
Evaluation of the serum enzymes Aspartate Transaminase Aminotransferase (AST) and Alanine Transaminase (ALT) in equines throughout the experimental period, demonstrating the effect of time between baseline (S0) and the last week of collection (S20). Serum AST rose from S5 and remained stable until the end of the study. ALT, on the other hand, presented a peak at S3 of the study and then a decrease in its enzymatic activity between S8 and S14.

Thumbnail

Fig.4.
Evaluation of serum enzymes Gamma Glutamyl Transferase (GGT) and Alkaline Phosphatase (AF) in equines throughout the experimental period, demonstrating the effect of time between baseline (S0) and the last week of collection (S20). GGT presented a first peak of serum elevation between S5 and S8 and a second peak between S13 and S20. AF presented higher serum activity at S5 and S20.

Thumbnail

Fig.5.
Values found for the enzyme Glutamate Dehydrogenase (GLDH) in equines throughout the experimental period, demonstrating the effect of time between baseline (S0) and the last week of collection (S20). The GLDH enzyme presented higher serum activity between S5 and S8. Then, there was a decrease in values in the final two weeks (S19 and S20).

Thumbnail

Table 1.
Mean ± standard deviation of the index evaluated by serum biochemistry at baseline (before the start of the immunization cycle), after the 1st, 2nd and 3rd immunization cycles of the experimental animals

Fig.1. Three-cycle immunization protocol for Bothrops venom used by the “Instituto Vital Brazil” in equines.

Fig.2. Evaluation of total protein and serum albumin in equines throughout the experimental period. Total proteins demonstrated an effect of time, with an increase starting at S5 and remaining stable until the end of the study at S20. Albumin, on the other hand, demonstrated that there was no effect of time between baseline (S0) and the last week of collection (S20).

Fig.3. Evaluation of the serum enzymes Aspartate Transaminase Aminotransferase (AST) and Alanine Transaminase (ALT) in equines throughout the experimental period, demonstrating the effect of time between baseline (S0) and the last week of collection (S20). Serum AST rose from S5 and remained stable until the end of the study. ALT, on the other hand, presented a peak at S3 of the study and then a decrease in its enzymatic activity between S8 and S14.

Fig.4. Evaluation of serum enzymes Gamma Glutamyl Transferase (GGT) and Alkaline Phosphatase (AF) in equines throughout the experimental period, demonstrating the effect of time between baseline (S0) and the last week of collection (S20). GGT presented a first peak of serum elevation between S5 and S8 and a second peak between S13 and S20. AF presented higher serum activity at S5 and S20.

Fig.5. Values found for the enzyme Glutamate Dehydrogenase (GLDH) in equines throughout the experimental period, demonstrating the effect of time between baseline (S0) and the last week of collection (S20). The GLDH enzyme presented higher serum activity between S5 and S8. Then, there was a decrease in values in the final two weeks (S19 and S20).

Table 1. Mean ± standard deviation of the index evaluated by serum biochemistry at baseline (before the start of the immunization cycle), after the 1st, 2nd and 3rd immunization cycles of the experimental animals

Serum biochemical index	Baseline	After 1st cycle of immunizations	After 2nd cycle of immunizations	After 3rd cycle of immunizations	Total
Urea (mg/dL)	34.0a ± 3.4	26.6b ± 2.7	30.0ab ± 4.2	30.0ab ± 1.2	28.8 ± 4.2
Creatinine (mg/dL)	1.3 ± 0.2	1.3 ± 0.3	1.3 ± 0.4	1.3 ± 0.3	1.3 ± 0.3
Total protein (g/dL)	7.8b ± 0.5	9.9a ± 0.4	10.1a ± 0.5	10.4a ± 1.0	9.0 ± 1.3
Albumin (g/dL)	2.5 ± 0.2	2.2 ± 0.1	2.4 ± 0.2	2.4 ± 0.4	2.4 ± 0.3
AST (IU/L)	140.4b ± 18.4	267.4a ± 34.5	326.4a ± 51.6	330.6a ± 49.9	254.7 ± 78.9
ALT (IU/L)	12.0a ± 0.7	6.6b ± 2.3	6.0b ± 1.9	8.0b ± 1.0	9.8 ± 5.1
GLDH (UI/L)	5.3b ± 1.8	7.2a ± 3.4	4.2ab ± 1.7	2.5ab ± 0.5	4.6 ± 2.1
GGT (IU/L)	4.6c ± 1.9	13.4b ± 4.4	21.6a ± 9.6	17.8ab ± 3.6	11.4 ± 7.4
FA (IU/L)	139.0b ± 20.8	252.0a ± 71.8	250.6a ± 82.5	266.6a ± 77.5	219.5 ± 78.3

^a,b
Distinct letters between columns indicate a significant difference according to the Tukey test (P<0.05).

Como citar

copiar

Colégio Brasileiro de Patologia Animal - CBPA Pesquisa Veterinária Brasileira, Caixa Postal 74.591, 23890-000 Rio de Janeiro, RJ, Brasil, Tel./Fax: (55 21) 2682-1081 - Rio de Janeiro - RJ - Brazil
E-mail: pvb@pvb.com.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Função hepática de éguas submetidas à inoculação de antígeno botrópico para produção de soro antiofídico

PlumX

Mensagem

[1] *Corresponding author: mariana_nahum@hotmail.com