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Stereoselective syntheses of (+)- isoretronecanol and (+)-5-epi-tashiromine via addition of chiral titanium (iv) enolates to cyclic n-acyliminium ions

Abstract

The stereoselective addition of the titanium (IV) enolates derived from (S)-4-isopropyl-N-4-chlorobutyryl-1,3-thiazolidine-2-thione (8) and from (S)-4-isopropyl-N-4-chloropentanoyl-1,3-thiazolidine-2-thione (9) to N-Boc-2-methoxypyrrolidine (5b) afforded the addition products (+)-10 and (+)-11 in 84% yield in both cases, as 8.6:1 and 10:1 diastereoisomeric mixtures, respectively. A three-step sequence allowed to convert these adducts to (+)-isoretronecanol (1) and (+)-5-epi-tashiromine (2) in 43% and 49% overall yield, respectively.

(+)-isoretronecanol; (+)-5-epi-tashiromine; asymmetric synthesis


ARTIGO

Stereoselective syntheses of (+)- isoretronecanol and (+)-5-epi-tashiromine via addition of chiral titanium (iv) enolates to cyclic n-acyliminium ions

Elaine Pereira; Conceição de Fátima Alves; Maria Alice Böckelmann; Ronaldo A. Pilli* * e-mail: pilli@iqm.unicamp.br

Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13084-971 Campinas-SP, Brazil

ABSTRACT

The stereoselective addition of the titanium (IV) enolates derived from (S)-4-isopropyl-N-4-chlorobutyryl-1,3-thiazolidine-2-thione (8) and from (S)-4-isopropyl-N-4-chloropentanoyl-1,3-thiazolidine-2-thione (9) to N-Boc-2-methoxypyrrolidine (5b) afforded the addition products (+)-10 and (+)-11 in 84% yield in both cases, as 8.6:1 and 10:1 diastereoisomeric mixtures, respectively. A three-step sequence allowed to convert these adducts to (+)-isoretronecanol (1) and (+)-5-epi-tashiromine (2) in 43% and 49% overall yield, respectively.

Keywords: (+)-isoretronecanol; (+)-5-epi-tashiromine; asymmetric synthesis.

INTRODUCTION

Pyrrolizidine alkaloids are structurally characterized by the presence of the 1-aza-bicyclo[3.3.0]octane ring system and are widely displayed in Nature being particularly ubiquitous in the genus Senecio (Compositae) where they occur mainly as esters or macrolactones of a necic base and a monocarboxylic or dicarboxylic acids. They may function as insect anti-feedants and are sequestered by some insects as part of their chemical defense weaponry. They represent probably the most common poisonous plants affecting livestock, wildlife and humans.1

Indolizidine alkaloids features the 1-aza-bicyclo[4.3.0]nonane ring system and have been isolated from several natural sources, including animals and plants.2 They display a variety of biological activities such as insecticidal, fungicidal and antibacterial activities and include some well known natural products such as castanospermine, gephyrotoxin, pumiliotoxins, allopumiliotoxins, securinine and indolizomycin, among others.

These alkaloids have become popular synthetic targets and have served as the testing ground to demonstrate the usefulness of synthetic methodologies, including those based on the use of N-acyliminium ions as the electrophilic species.3

In the past, we have explored the addition of carbon nucleophiles such as allyltrimethylsilane, silyloxydienes and boron enolates derived from chiral N-acyl-1,3-oxazolidin-2-ones to chiral N-acyliminium ion precursors to develop efficient approaches to enantiomerically pure pyrrolizidine, indolizidine and quinolizidine alkaloids.4

This last approach, which attracted our interest due to the possibility to carry out chirality transfer from the chiral N-acyl-1,3-oxazolidin-2-one precursors to achiral N-acyliminium ions, followed by the recovery of the chiral auxiliary, was pioneered by Fuentes et al. who described the addition of chiral 1,3-oxazolidin-2-ones to cyclic acylimines5 and Nagao and coworkers6 who reported on the addition of tin(II) enolates of chiral thiazolidine-2-thiones to N-acylimines and N-acyliminium ions which led to the total synthesis of the pyrrolizidine necine base (-)-supinidine.7 We have also described the addition of boron and titanium (IV) enolates derived from chiral N-acyl-1,3-oxazolidin-2-ones to 5- and 6-membered N-acyliminium ions.8

Isoretronecanol (1), a pyrrolizidine alkaloid which is found in Nature in both enantiomeric forms, has been isolated from Planchonella, Hammarbya, Phalaenopsis and Heliotropium species, and it has been synthesized in optically pure form several times in the past.9 Among the indolizidine alkaloids, (+/-)-tashiromine and its epimer (+/-)-5-epi-tashiromine (2) have been prepared on several occasions but only a few reports on the synthesis of these alkaloids in enantiomerically pure form appeared (Figure 1).10


Recently, we have disclosed our results on the stereoselective addition of the preformed titanium (IV) enolate derived from N-4-chlorobutyryl-1,3-thiazolidine-2-thione to N-Boc-2-methoxypyrrolidine which allowed the preparation of the pyrrolizidine alkaloid (+)-isoretronecanol (1) in four steps and 36% overall yield11 and herein we describe details of our work and extend this methodology to the synthesis of (+)-5-epi-tashiromine (2), an indolizidine alkaloid which was initially isolated from Maackia tashiroi12 and recently from Crotalaria species.13

RESULTS AND DISCUSSION

Our approach initially focused on the addition of the titanium (IV) enolates derived from N-chlorobutyryl and N-chloropentanoyl-1,3-oxazolidin-2-ones (3 and 4, respectively) to N-tert-butoxycarbonyl-2-ethoxypyrrolidine (5a). The titanium(IV) enolates were generated by treatment of a cooled (0 ºC) CH2Cl2 soln. of oxazolidinones 3 and 4 with TiCl4 to produce a deeply colored reddish brown soln. of the corresponding enolate which was treated with a CH2Cl2 soln. of 5a at –23 ºC to afford the corresponding adducts in good yields (82 and 81% yield, respectively) as a single diastereoisomer, as determined by 1H-NMR analyses of the crude reaction mixture (Scheme 1). The absolute configuration of the newly formed stereogenic centers in adducts (+)-6 and (+)-7 was determined to be (2R, 2'R) after X-ray diffraction analysis of a monocrystal of adduct (-)-7.14


The stereochemical outcome favoring the (2R,2'R) configuration in 6 and 7 may be rationalized via the interaction between the sterically less encumbered Si face of the Z-configured and internally chelated titanium(IV) enolate derived from 3 and 4 and Si face of the N-acyliminium ion formed in situ upon addition of N-Boc-2-methoxy pyrrolidine (5a) to the red colored CH2Cl2 solution of the enolate (Figure 2).


Despite the efficiency of the above process which provided good yield and stereocontrol in the preparation of 2-substituted pyrrolidines, removal of the chiral auxiliary under mild reaction conditions (such as LiOH/H2O2/THF, LiBH4/MeOH or PhCH2SLi/THF) proved to be difficult as low yields of the corresponding carboxylic acid, alcohol or thioester were observed due to the competitive attack at the endocyclic carbonyl.15

In order to circumvent this problem, we investigated the reaction of the titanium(IV) enolate derived from N-chlorobutyryl and N-chloropentanoyl-1,3-thiazolidin-2-thiones (8 and 9, respectively) with N-tert-butoxycarbonyl-2-methoxypyrrolidine (5b) as the cleavage of the exocyclic N-acyl bond in the N-acyl-1,3-thiazolidine-2-thiones is favored when compared to the corresponding N-acyl-1,3-oxazolidine-2-ones.16

Upon treatment of a yellow solution of N-chlorobutyryl- and N-chloropentanoyl-1,3-thiazolidin-2-thiones (8 and 9, respectively) in CH2Cl2 with 1.1 equiv. of TiCl4 at 0 ºC, followed by addition of 1.1 equiv. of diisopropylethylamine, a redish brown color developed. After addition of a pre-cooled CH2Cl2 solution of N-Boc-2-methoxypyrrolidine (5b) at –23 ºC, the corresponding adducts 10 and 11 were isolated in 84% yield in both cases, after column chromatography in silica gel, as a 8.6:1 and 10:1 diastereoisomeric ratio, respectively, as determined by HPLC analyses on the crude mixture.17 These adducts were fully characterized by HRMS, IR, 1H- and 13C-NMR spectroscopies, as well as specific optical rotation.

The configuration of the newly generated stereogenic centers in adducts 6 and 10 were shown to be identical after their conversion to the same alcohol 12 upon treatment with NaBH4. The same identity was observed for adducts 7 and 11 which afforded alcohol 13 after reductive cleavage under the same experimental conditions. Their absolute configurations were tentatively assigned based on our previous work8 and were eventually confirmed after their conversion to the pyrrolizidine alkaloid (+)-isoretronecanol (1) and to the indolizidine alkaloid (+)-5-epi-tashiromine (2), as depicted below.

Upon treatment of 2-substituted pyrrolidines 10 and 11 with LiBH4 in THF/MeOH, an inseparable mixture of alcohols 12 and 13, respectively, and (S)-isopropyl-1,3-thiazolidine-2-thione was formed (Scheme 2). Their separation proved not to be necessary at this step as deprotection of the nitrogen with trifluoroacetic acid, followed by addition of an excess of saturated NaHCO3, provided (+)-isoretronecanol (1) and 5-epi-tashiromine (2), in 43 and 49% overall yield, respectively, after silica gel chromatography. The chiral auxiliary was recovered in 66 and 70% yield, respectively.


Synthetic (+)-isoretronecanol (1) and (+)-5-epi-tashiromine (2) were characterized by comparison of their spectroscopic and optical rotation data with those described in the literature.10,18 The route described above compares favourably (4 steps and 32% and 37% overall yield from the corresponding N-acyl thiazolidin-2-thiones 8 and 9, respectively) with the most efficient methods already described in the literature for the synthesis of these alkaloids in optically pure form.9,10

EXPERIMENTAL

Reagent grade chemicals were used as purchased except when noticed otherwise. THF was distilled from sodium/benzophenone immediately prior to use. Dichloromethane, diisopropylethylamine and titanium tetrachloride were dried over calcium hydride and distilled immediately prior to use. Reactions involving air or water sensitive reagents were carried out in previously flame-dried flasks and under an argon atmosphere.

The reaction progress was followed by TLC on silica gel plates (0.20 mm) and stained under iodine vapor, potassium permanganate soln. or Dragendorf reagent. Column chromatography separations were performed in silica gel (70-230 and 230-400 Mesh).

The NMR spectra were measured in CDCl3 soln. in a 7.0 Tesla spectrometer at 25 ºC. The chemical shifts are reported in part per million downfield from tetramethylsilane (1H-NMR) or residual chloroform (13C-NMR) and data are reported as follows: chemical shift, integrals, multiplicity (s = singlet, d = doublet, dd = double dublet, t = triplet, q = quartet, qt = quintuplet, m = multiplet), br (broad signal) and coupling constants. Melting points were measured in an open capillary and were not corrected.

N-tert-butoxycarbonyl 2-ethoxy pyrrolidine (5a) and N-tert-butoxycarbonyl 2-methoxy pyrrolidine (5b) were prepared according to the procedure described by Shono and coworkers.19 The preparation of (S)-3-isopropyl-1,3-thiazolidine-2-thione was carried out according to the procedure by Corre and coworkers.20

(S)-4-Benzyl-3-(4-chlorobutanoyl)-1,3-oxazolidin-2-one (3)

To a soln. of commercially available (S)-benzyl-1,3-oxazolidin-2-one (3.0 g, 17 mmol) in THF (34 mL) at 78 ºC was added dropwise a soln. of n-BuLi in hexanes (1.96 M, 9.5 mL, 18.6 mmol). The reaction mixture was stirred at 78 ºC for 30 min and 4-chlorobutanoyl chloride (2.86 g, 20.3 mmol). After consumption of the starting material as monitored by TLC, a satd. aq. soln. of NH4Cl (10 mL) was added, the aqueous phase was extracted with ether (3 x 10 mL) and the combined organic phase was dried over MgSO4. The crude reaction mixture was purified by silica gel chromatography (10% EtOAc in hexanes) to afford (S)-3 (4.40 g, 15.6 mmol) in 92% yield as a pale yellow oil ([a]D20 = +73.0 (c 4.3, CH2Cl2). IR (neat): 3028, 2964, 2924, 1778, 1693, 1390, 1213 (br), 762, 704 cm-1; 1H-NMR (CDCl3): d 2.17 (qt, 2H, J =6.5 Hz), 2.78 (dd, 1H, J =13.3 and 9.6 Hz), 3.12 (m, 2H), 3,27 (dd, 1H, J = 13.5 and 3,3Hz), 3,66 (t, 2H, J =6.5 Hz), 4.20 (m, 2H), 4.68 (m, 1H), 7.32 (m, 5H); 13C-NMR (CDCl3): d 26.9, 32.7, 37.8, 44.0, 55.1, 66.3, 127.4, 128.9, 129.4, 135.1, 153.4, 172.1. Elemental analyses calcd. for C14H16ClNO3: %C 59.68, %H 5.72, %N 4.97. Found: %C 59.70, %H 5.68, %N 4.97.

(S)-4-Benzyl-3-(5-chloropentanoyl)-1,3-oxazolidin-2-one (4)

The same procedure as described above for (S)-3 was employed to provide (S)-4 as a white solid (mp 66-67 ºC, crystals from EtOAc-hexanes) in 98% yield. ([a]D20 = +63.0 (c 3.1, CH2Cl2). IR (KBr): 3028, 2956, 1780, 1699, 1388, 1213 (br), 762 cm-1; 1H-NMR (CDCl3): d 1.86 (m, 4H), 2.77 (dd, 1H, J =13.5 and 9.5 Hz),2.95 (m, 2H), 3.29 (dd, 1H, J = 13.2 and 3,3 Hz), 3,58 (m, 2H), 4.19 (m, 2H), 4.68 (m, 1H), 7.31 (m, 5H); 13C-NMR (CDCl3): d 21.6, 31.9, 34.8, 38.0, 44.7, 55.2, 66.4, 127.6, 129.2, 129.7, 135.5, 153.8, 173.0. HRMS (EI): calcd. for C15H18NO3Cl: 295.0975. Found: 295.0972.

(S)-4-Isopropyl-3-(4-chlorobutanoyl)-1,3-thiazolidin-2-thione (8)

A soln. of (S)-4-isopropyl-1,3-thiazolidin-2-thione (1.00 g, 6.20 mmol) in THF (2 mL) was added to a suspension of 60% w/w NaH (0.27 g, 6.7 mmol)) in THF (1 mL) at 0 ºC. The reaction mixture was stirred 10 min at 0 ºC and then freshly distilled 4-chlorobutanoyl chloride (0.958 g, 6.82 mmol) was added dropwise. The reaction mixture was kept at 0 ºC for 10 min and then stirred at rt for 1 h. After addition of aq. HCl (10% v/v, 5 mL) and extraction with EtOAc (3 x 5 mL), the combined organic extracts were washed with brine (2 x 5 mL), dried over Na2SO4 and the solvent was removed under reduced pressure. The crude product was purified by chromatography on silica gel (5% EtOAc-hexanes) to afford (S)-8 (1.51 g, 5.40 mmol) in 92% yield, as a yellow oil. ([a]D20 = +363.0 (c 2.0, CHCl3). IR (neat): 2963, 1696, 1469, 1367, 1276, 750 cm-1; 1H-NMR (CDCl3): d 0.97 (d, 3 H, J = 7.0 Hz), 1.07 (d, 3H, J = 7.0 Hz), 2.05-2.26 (m, 2H), 2.28-2.44 (m, 1H), 3,03 (dd, 1H, J = 11.3 and 1.1 Hz), 3.26-3.37 (m, 1H),, 3,59-3.60 (m, 2H), 3.62 (t, 2H, J = 6.4 Hz), 5.15 (ddd, 1H, J = 7.7, 6.4, 1.1 Hz); 13C-NMR (CDCl3): d 17.9, 19.2, 27.8, 30.6, 30.9, 35.7, 44.1, 71.6, 172.6, 202.4. HRMS (EI): calcd. for C10H16NOS2Cl: 265.0362. Found: 265.0365.

(S)-4-Isopropyl-3-(5-chloropentanoyl)-1,3-thiazolidin-2 -thione (9)

The same procedure as described above for (S)-8 was employed to provide (S)-9 as yellow oil in 96% yield. ([a]D20 = +344.0 (c 2.0, CHCl3). IR (neat): 2962, 1694, 1313, 1259, 1160 (br), 1037 cm-1; 1H-NMR (CDCl3): d 0.97 (d, 3 H, J = 7.0 Hz), 1.06 (d, 3H, J = 6.6 Hz), 1.73-1.92 (m. 4H), 2.30-2.41 (m, 1H), 3,03 (dd, 1H, J = 11.3 and 0.9 Hz), 3.13-3.21 (m, 1H), 3,35-3.45 (m, 1H), 3.49-3.58 (m, 3H), 5.14-5.19 (m, 1H); 13C-NMR (CDCl3): d 17.6, 18.9, 22.1, 30.3, 30.7, 31.7, 37.3, 44.5, 71.4, 173.2, 202.7. HRMS (EI): calcd. for C11H18NOS2Cl: 279.0518. Found: 279.0517.

tert-Butyl (2R,1'R)-{2'-oxo-2'-[(4S)-4-benzyl-2-oxo-1,3 -oxazolan-3-yl]-1'-(2'-chloroethyl)}-2-ethylazolane-1 -carboxylate (6)

To a soln. of TiCl4 (0.12 mL, 1.1 mmol) in CH2Cl2 (2.0 mL) at 23 ºC was added dropwise a soln. of (S)-3 (0.281 g, 1.00 mmol) in CH2Cl2 (3.0 mL), followed by the addition of diisopropylethylamine (0.20 mL, 1.1 mmol). The deep reddish brown mixture obtained was stirred at 23 ºC for 1 h and then it was added dropwise a soln. of N-tert-butoxycarbonyl-2-ethoxypyrrolidine (5a, 0.236 g, 1.1 mmol) in CH2Cl2 (5 mL). After consumption of the starting material (monitored by TLC), satd. NH4Cl (2.0 mL) was added and the aqueous phase was extracted with CH2Cl2 (3 x 10 mL). The combined organic phase was dried over MgSO4 and the solvent was removed under reduced pressure. The crude product was purified by flash chromatography on silica gel (15% EtOAc-hexanes) to provide 6 (0.370 g, 0.82 mmol) in 82% yield, as a white solid (mp 115.4-116.4 ºC). ([a]D20 = +51.0 (c 1.5, CH2Cl2). IR (KBr): 2970, 1782, 1691, 1445, 1390, 1299, 1207, 1167, 1109, 756 cm-1; 1H-NMR (CDCl3, 50 ºC): d 1.45 (s, 9 H), 1.70-2.05 (m, 5H), 2.25-2.48 (m, 1H), 2.61 (dd, 1H, J = 10.5 and 10.3 Hz), 3.20-3.35 (m, 1H), 3.35-3.50 (m, 2H), 3.50-3.65 (m, 2H), 4.05-4.20 (m, 2H), 4.20-4.40 (m, 1H), 4.40-4.60 (s, 1H, br), 4.60-4.80 (m, 1H), 7.25 (m, 5H). 13C-NMR (CDCl3, 50 ºC): d 23.7, 28.6, 28.9, 31.0, 38.3, 43.1, 44.5, 47.4, 55.7, 58.4, 66.2, 80.0, 127.3, 129.0, 129.4, 135.8, 153.0, 155.0, 173.5. HRMS (EI): calcd. for C18H22N2O3 Cl (M+/. C4H9: 349.1319. Found: 349.1317.

tert-Butyl (2R,1'R)-{2'-oxo-2'-[(4S)-4-benzyl-2-oxo-1,3 -oxazolan-3-yl]-1'-(3'-chloropropyl)}-2-ethylazolane-1 -carboxylate (7)

The same procedure as described above for (+)-6 afforded (+)-7 in 81% yield, after purification by flash chromatography on silica gel (15% EtOAc/hexanes) as a pale yellow oil. ([a]D20 = +49.6 (c 3.6, CH2Cl2). IR (neat): 2974, 1780, 1689, 1483, 1388, 1299, 1205, 1165, 1105, 762 cm-1; 1H-NMR (CDCl3, 50 ºC): d 1.44 (s, 9 H), 1.20-1.50 (m, 8H), 2.60 (dd, 1H, J = 13.3 and 10.4 Hz), 3.20-3.40 (m, 1H), 3.40-3.65 (m, 2H), 3.50 (t, 2H, J = 6.4 Hz), 4.12 (m, 2H), 4.20-4.35 (s, br, 2H), 4.60-4.80 (s, 1H), 7.29 (m, 5H). 13C-NMR (CDCl3, 50 ºC): d 21.6, 23.6, 28.5, 28.7, 30.7, 38.2, 44.5, 45.7, 47.1, 55.6, 58.7, 66.1, 79.8, 127.2, 128.9, 129.3, 135.7, 153.1, 154.9, 174.0. HRMS (EI): calcd. for C19H24N2O3 Cl (M+/. C4H9): 363.1475. Found: 363.1475.

tert-Butyl (2R,1'R)-{2'-oxo-2'-[(4S)-4-isopropyl-2-thio-1,3 -thiazolan-3-yl]-1'-(2'-chloroethyl)}-2-ethylazolane-1 -carboxylate (10)

To a soln. of TiCl4 (0.12 mL, 1.1 mmol) in CH2Cl2 (3.0 mL) at 0 ºC was added a soln. of (+)-8 (0.265 g, 1.0 mmol) in CH2Cl2 (2.0 mL), followed by the addition of diisopropylethylamine (0.20 mL, 1.1 mmol). The redish brown mixture was kept at 0 oC for 1 h and then it was cooled to 23 ºC. A previously cooled soln. of 2-methoxy-N-tert-butoxycarbonyl pyrrolidine (5b, 0.221 g, 1.1 mmol) was added dropwise and the reaction mixture was stirred for 1 h at 23 ºC. After addition of satd. soln. of NH4Cl (5.0 mL) and brine (5.0 mL), the aqueous phase was extracted with CH2Cl2 (3 x 10 mL) and the combined organic phase was dried over MgSO4. After evaporation under reduced pressure, the crude product was purified by chromatography on silica gel (5% AcOEt- Hexanes) to afford (+)-10 (0.376 g, 0.80 mmol) in 84% yield as a yellow oil (8.6:1 diastereoisomeric ratio). Data for the major isomer: ([a]D20 = +222.0 (c 1.0, CHCl3). IR (neat): 2969, 1693, 1392, 1366, 1306, 1252, 1166 cm-1; 1H-NMR (CDCl3, 60 ºC): d 0.98 (d, 3 H, J= 7.0 Hz), 1.08 (d, 3 H, J= 7.0 Hz), 1.47 (s, 9H), 1.72-2.01 (m. 5H), 2.09-2.38 (m, 2H), 3,03 (dd, 1H, J = 11.5 and 1.3 Hz), 3.15-3.30 (m, 1H), 3.4-3.6 (m, 4H), 4.38 (m, 1H), 4,92-5.00 (m, 1H), 5.11-5.18 (m, 1H); 13C-NMR (CDCl3): d 17.3, 19.0, 23.8, 28.3, 28.5, 30.3, 31.1, 31.5, 43.0, 44.7, 47.4, 58.0, 71.8, 79.7, 154.6, 174.0, 202.0. HRMS (EI): calcd. for C19H31N2O3 S2Cl: 434.1465. Found: 434.1462.

tert-Butyl (2R,1'R)-{2'-oxo-2'-[(4S)-4-isopropyl-2-thio-1,3 -thiazolan-3-yl]-1'-(3'-chloropropyl)}-2-ethylazolane-1 -carboxylate (11)

The same procedure as described for (+)-10 was employed to afford (+)-11 in 84% yield, after chromatography on silica gel (5% EtOAc-hexanes), as a yellow oil (10:1 diastereoisomeric ratio). Data for the major isomer: ([a]D20 = +248.0 (c 2.0, CHCl3). IR (neat): 2967, 1693, 1392, 1366, 1306, 1253, 1166 cm-1; 1H-NMR (CDCl3, 60 ºC): d 0.95 (d, 3H, J=7.0 Hz), 1.06 (, 3H, J=7.0 Hz), 1.47 (s, 9H), 1.61-2.00 (m. 8H), 2.27-2.38 (m, 1H), 2.98-3,02 (m, 1H), 3.19-3.39 (m, 1H), 3.42-3.53 (m, 4H), 4,9-4.94 (m, 1H), 5.08-5.19 (m, 2H); 13C-NMR (CDCl3): d 17.4, 19.2, 23.9, 26.5, 28.4, 28.7, 30.0, 30.7, 31.1, 44.7, 46.0, 47.3, 58.4, 72.0, 79.7, 154.7, 174.9, 202.2. HRMS (EI): calcd. for C20H33N2O3 S2Cl: 448.1621. Found: 448.1611.

(+)-Isoretronecanol (1)

To a soln. of (+)-10 (0.146 g, 0.32 mmol) in THF (2.0 mL) at 0 ºC was added methanol (0.02 mL), followed by the addition of a soln. of LiBH4 (0.023 g, 1.18 mmol) in THF (2.0 mL). The mixture was stirred 1 h at 0 ºC and a satd. soln. of NH4Cl (5.0 mL) was added and extracted with ether (3 x 5.0 mL). The organic phase was dried over MgSO4, the solvent was evaporated under reduced pressure to provide a crude mixture of alcohol 12 and (S)-4-isopropyl-1,3-thiazolidin-2-thione (0.130 g) which was taken up in CH2Cl2 (12.0 mL) and added to a mixture of trifluoroacetic acid (0.27 mL, 3.5 mmol) containing triethylsilane (0.050 mL, 0.30 mmol) at 0 ºC. The reaction mixture was stirred at rt for 24 h and it was quenched by the addition of satd. aq. NaHCO3 (2.0 mL) and excess solid NaHCO3 to reach pH 9.0. The reaction mixture was vigorously stirred at rt for 16 h, filtered to separate the solids and the phases were separated. The organic phase was dried over MgSO4 and after removal of the solvent under reduced pressure, the crude reaction mixture was purified by column chromatography on neutral alumina: elution with CH2Cl2 provided (S)-4-isopropyl-1,3-thiazolidin-2-thione (0.034 g, 0.21 mmol) in 66% yield and elution with 10% MeOH/CHCl3 provided (+)-isoretronecanol (1) in 43% yield (0.019 g, 0.14 mmol) as a pale yellow oil. [a]D20 = +71.0 (c 1.3, EtOH); lit.21: [a]D20 = +71.7 (c 1.0, EtOH). IR (neat): 3384, 2954, 2870, 1454, 1216, 1107, 1072, 1032, 771 cm-1; 1H-NMR (CDCl3): d 1.30-1.45 (m, 1H), 1.45-1.60 (m, 1H), 1.4-2.0 (m, 4H), 2.35-2.60 (m, 2H), 2.62 (s,1H, br), 3.02 (s,1H, br), 3,20 (s, 1H,br), 3..52 (s, 1H, br), 3.5-3.7 (m, 2H), 3,90 (s, 1H, br); 13C-NMR (CDCl3): d 25.9, 26.5, 27.2, 44.2, 54.0, 55.6, 63.1, 66.4 (Literature data18: d 25.9, 26.6, 27.3, 43.9, 54.0, 55.6, 63.3, 66.8). HRMS (EI): calcd. for C8H15NO: 141.1154. Found: 141.1154.

(+)-5-epi-Tashiromine (2)

To a soln. of (+)-11 (0.059 g, 0.13 mmol) in THF (2.0 mL) at 0 ºC was added methanol (0.02 mL), followed by the addition of a soln. of LiBH4 (0.010 g, 0.50 mmol) in THF (2.0 mL). The mixture was stirred 1 h at 0 ºC and a satd. soln. of NH4Cl (5.0 mL) was added and extracted with ether (3 x 5.0 mL). The organic phase was dried over MgSO4, the solvent was evaporated under reduced pressure to provide a crude mixture of alcohol 13 and (S)-4-isopropyl-1,3-thiazolidin-2-thione which was taken up in CH2Cl2 (12.0 mL) and added to a mixture of trifluoroacetic acid (0.17 mL, 2.2 mmol) containing triethylsilane (0.030 mL, 0.30 mmol) at 0 ºC. The reaction mixture was stirred at rt for 24 h and it was quenched by the addition of satd. aq. NaHCO3 (2.0 mL) and excess solid NaHCO3 to reach pH 9.0. The reaction mixture was vigorously stirred at rt for 16 h, filtered to separate the solids and the phases were separated. The organic phase was dried over MgSO4 and after removal of the solvent under reduced pressure, the crude reaction mixture was purified by column chromatography on neutral alumina: elution with CH2Cl2 provided (S)-4-isopropyl-1,3-thiazolidin-2-thione (0.015 g, 0.09 mmol) in 70% yield and elution with 10% MeOH/CHCl3 provided (+)-5-epi-tashiromine (2) in 49% yield (0.010 g, 0.14 mmol) as a pale yellow oil. [a]D20 = +15.0 (c 1.3, EtOH). (+)-5-epi-tashiromine.HCl: [a]D20 = +28.0 (c 0.45, EtOH); liter. lit.10: [a]D20 = +29.1 (c 0.45, EtOH). IR (neat): 3109, 3018, 1645, 1396, 1250, 1074, 1041 cm-1; 1H-NMR (CDCl3): d 1.25-1.91 (m, 9H), 1.92-2.08 (m, 3H), 2.45 (s, br, 1H), 3.02-3.06 (m, 2H), 3.72 (d, 1H, J =10.7 Hz), 4.12-4;13 (m, 1H); 13C-NMR (CDCl3): d 20.6, 23.1, 25.2, 29.7, 35.5, 52.9, 54.3, 65.4, 66.4 (Literature data10: d 20.7, 23.2, 25.6, 29.7, 35.3, 53.2, 54.4, 65.6, 66.7). HRMS (EI): calcd. for C8H15NO: 141.1154. Found: 141.1154.

ACKNOWLEDGMENTS

The authors would like to thank FAPESP (Fundação de Amparo à Pesquisa no Estado de São Paulo) for financial support and fellowship and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for research fellowship.

REFERENCES AND NOTES

1. Fu, P. P.; Wia, Q. S.; Lin, G.; Chou, M. W.; Drug Metabolism Rev. 2004, 36, 1.

2. For a recent review on indolizidine and quinolizidine alkaloids, see: Michael, J. P.; Nat. Prod. Rep. 2007, 24, 191.

3. For recent reviews on the chemistry of N-acyliminium ions, see: Speckamp, W. N.; Moolenaar, M. J.; Tetrahedron 2000, 56, 3817; Pilli, R. A.; Rosso, G. B. In Science of Synthesis. Houben-Weyl Methods of Molecular Transformations; Padwa, A., ed.; Stuttgart: Thieme, 2004, vol. 27, p. 375-440.

4. Pilli, R. A.; Dias, L. C.; Maldaner, A. O.; J. Org. Chem. 1995, 60, 717; Pilli, R. A.; Russowsky, D.; J. Org. Chem. 1996, 61, 3187; Pilli, R. A.; Santos, L. S.; Tetrahedron Lett. 2001, 42, 6999; Pilli, R. A.; Zanotto, P. R.; Böckelmann, M. A.; Tetrahedron Lett. 2001, 42, 7003; Klitzke, C. F.; Pilli, R. A.; Tetrahedron Lett. 2001, 42, 5605; Santos, L. S.; Pilli, R. A.; J. Braz. Chem. Soc. 2003, 14, 982.

5. Fuentes, L. M.; Shinkai, I.; Salzmann, T. N.; J. Am.Chem. Soc. 1986, 108, 4675.

6. Nagao, Y.; Kumagai, T.; Tamai, S.; Abe, T.; Kuramoto, Y.; Taga, T.; Aoyagi, S.; Nagase, Y.; Ochiai, M.; Inoue, Y.; Fujita, E.; J. Am. Chem. Soc. 1986, 108, 4673; Nagao, Y.; Dai, W-M.; Ochiai, M.; Tsukagoshi, S.; Fujita, E.; J. Am. Chem. Soc. 1988, 110, 289; Nagao, Y.; Daí, W-M.; Ochiai, M.; Tsukagoshi, S.; Fujita, E.; J. Org. Chem. 1990, 55, 1148.

7. Nagao, Y.; Daí, W-M.; Ochiai, M.; Tetrahedron Lett. 1988, 29, 6133; Nagao, Y.; Daí, W-M.; Ochiai, M.; Shiro, M.; Tetrahedron 1990, 46, 6361.

8. Pilli, R. A.; Alves, C. F.; Böckelmann, M. A.; Mascarenhas, Y. P.; Nery, J. G.; Vencato, I.; Tetrahedron Lett. 1999, 40, 2891; Pilli, R. A.; Böckelmann, M. A.; Alves, C. F.; J. Braz. Chem. Soc. 2001, 12, 634.

9. For recent syntheses of (+)- and (-)-isoretronecanol, see: Bertrand, S.; Hoffmann, N.; Pete, J-P.; Eur. J. Org. Chem. 2000, 2227; Ledoux, S.; Machalant, E.; Celerier, J-P.; Lhommet, G.; Tetrahedron Lett. 2001, 42, 5397; Dieter, R. K.; Chen, N.; Watson, R. T.; Tetrahedron 2005, 61, 3221.

10. Ha, D-C.; Park, S-H.; Choi, K-S.; Yun, C-S.; Bull. Kor. Chem. Soc. 1998, 19, 728; Banwell, M. G.; Beck, D. A. S.; Smith, J. A.; Org. Biomol. 2004, 2, 157; Gage, J. L.; Branchard, B. P.; Tetrahedron Lett. 1997, 28, 7007; Agami, C.; Dechoux, L.; Hebbe, S.; Ménard, C.; Tetrahedron 2004, 60, 5433; Dieter, R. K.; Chen, N.; Watson, R. T.; Tetrahedron 2005, 61, 3221.

11. Pereira, E.; Alves, C. F.; Böckelmann, M. A.; Pilli, R. A.; Tetrahedron Lett. 2005, 46, 2691.

12. Ohmiya, S.; Kubo, H.; Otomasu, H.; Saito, K.; Murakoshi, I.; Heterocycles 1999, 30, 537.

13. Asres, K.; Sporer, F.; Wink, M.; Biochem. Syst. Ecol. 2004, 32, 915.

14. Alves, C. F.; PhD Thesis, State University of Campinas, 1998.

15. Damon, R. E.; Coppola, G. M.; Tetrahedron Lett. 1990, 31, 2849; Evans, D. A.; Black, W. C.; J. Am. Chem. Soc. 1993, 115, 4497; Evans, D. A.; Johnson, J. S.; J. Org. Chem. 1997, 62, 786; Evans, D. A.; Wu, L. D.; Wiener, J. J. M.; Johnson, J. S.; Ripin, D. H. B.; Tedrow, J. S.; J. Org Chem. 1999, 64, 6411.

16. Nagao, Y.; Yagi, M.; Ikede, T.; Fujita, E.; Tetrahedron Lett. 1982, 23, 201; Crimmins, M. T.; King, B. W.; Tabet, E. A.; J. Am. Chem. Soc. 1997, 119, 7883; Crimmins, M. T.; King, B. W.; Tabet, E. A.; Chaudhary, K.; J. Org. Chem. 2001, 66, 894.

17. HPLC analyses on the crude mixtures of adducts 10 and 11 were carried out with Hypersil column (5 mm, 200 x 4.6 mm) and 5% hexanes-isopropanol (v/v) mixture as the eluent.

18. David, O.; Blot, J.; Bellec, C.; Fargeau-Bellassoued, M-C.; Haviari, G.; Célérier, J-P.; Lhommet, G.; Gramain, J-C.; Gardette, D.; J. Org. Chem. 1999, 64, 3122.

19. Shono, T.; Matsumura, Y.; Tsubata, K.; Org. Synth. 1985, 63, 206.

20. Corre, M. L.; Toupet, L.; Delaunay, D.; J. Org. Chem. 1995, 60, 6604.

21. Robins, D. J.; Sakdarat, S.; Chem. Commun. 1979, 1181

Recebido em 4/1/08; aceito em 14/3/08; publicado na web em 9/4/08

  • 1. Fu, P. P.; Wia, Q. S.; Lin, G.; Chou, M. W.; Drug Metabolism Rev. 2004, 36, 1.
  • 2. For a recent review on indolizidine and quinolizidine alkaloids, see: Michael, J. P.; Nat. Prod. Rep 2007, 24, 191.
  • 3. For recent reviews on the chemistry of N-acyliminium ions, see: Speckamp, W. N.; Moolenaar, M. J.; Tetrahedron 2000, 56, 3817;
  • Pilli, R. A.; Rosso, G. B. In Science of Synthesis. Houben-Weyl Methods of Molecular Transformations; Padwa, A., ed.; Stuttgart: Thieme, 2004, vol. 27, p. 375-440.
  • 4. Pilli, R. A.; Dias, L. C.; Maldaner, A. O.; J. Org. Chem. 1995, 60, 717;
  • Pilli, R. A.; Russowsky, D.; J. Org. Chem. 1996, 61, 3187;
  • Pilli, R. A.; Santos, L. S.; Tetrahedron Lett. 2001, 42, 6999;
  • Pilli, R. A.; Zanotto, P. R.; Böckelmann, M. A.; Tetrahedron Lett. 2001, 42, 7003;
  • Klitzke, C. F.; Pilli, R. A.; Tetrahedron Lett. 2001, 42, 5605;
  • Santos, L. S.; Pilli, R. A.; J. Braz. Chem. Soc. 2003, 14, 982.
  • 5. Fuentes, L. M.; Shinkai, I.; Salzmann, T. N.; J. Am.Chem. Soc. 1986, 108, 4675.
  • 6. Nagao, Y.; Kumagai, T.; Tamai, S.; Abe, T.; Kuramoto, Y.; Taga, T.; Aoyagi, S.; Nagase, Y.; Ochiai, M.; Inoue, Y.; Fujita, E.; J. Am. Chem. Soc. 1986, 108, 4673;
  • Nagao, Y.; Dai, W-M.; Ochiai, M.; Tsukagoshi, S.; Fujita, E.; J. Am. Chem. Soc. 1988, 110, 289;
  • Nagao, Y.; Daí, W-M.; Ochiai, M.; Tsukagoshi, S.; Fujita, E.; J. Org. Chem. 1990, 55, 1148.
  • 7. Nagao, Y.; Daí, W-M.; Ochiai, M.; Tetrahedron Lett. 1988, 29, 6133;
  • Nagao, Y.; Daí, W-M.; Ochiai, M.; Shiro, M.; Tetrahedron 1990, 46, 6361.
  • 8. Pilli, R. A.; Alves, C. F.; Böckelmann, M. A.; Mascarenhas, Y. P.; Nery, J. G.; Vencato, I.; Tetrahedron Lett. 1999, 40, 2891;
  • Pilli, R. A.; Böckelmann, M. A.; Alves, C. F.; J. Braz. Chem. Soc. 2001, 12, 634.
  • 9. For recent syntheses of (+)- and (-)-isoretronecanol, see: Bertrand, S.; Hoffmann, N.; Pete, J-P.; Eur. J. Org. Chem. 2000, 2227;
  • Ledoux, S.; Machalant, E.; Celerier, J-P.; Lhommet, G.; Tetrahedron Lett. 2001, 42, 5397;
  • Dieter, R. K.; Chen, N.; Watson, R. T.; Tetrahedron 2005, 61, 3221.
  • 10. Ha, D-C.; Park, S-H.; Choi, K-S.; Yun, C-S.; Bull. Kor. Chem. Soc. 1998, 19, 728;
  • Banwell, M. G.; Beck, D. A. S.; Smith, J. A.; Org. Biomol 2004, 2, 157;
  • Gage, J. L.; Branchard, B. P.; Tetrahedron Lett. 1997, 28, 7007;
  • Agami, C.; Dechoux, L.; Hebbe, S.; Ménard, C.; Tetrahedron 2004, 60, 5433;
  • Dieter, R. K.; Chen, N.; Watson, R. T.; Tetrahedron 2005, 61, 3221.
  • 11. Pereira, E.; Alves, C. F.; Böckelmann, M. A.; Pilli, R. A.; Tetrahedron Lett. 2005, 46, 2691.
  • 12. Ohmiya, S.; Kubo, H.; Otomasu, H.; Saito, K.; Murakoshi, I.; Heterocycles 1999, 30, 537.
  • 13. Asres, K.; Sporer, F.; Wink, M.; Biochem. Syst. Ecol 2004, 32, 915.
  • 14. Alves, C. F.; PhD Thesis, State University of Campinas, 1998.
  • 15. Damon, R. E.; Coppola, G. M.; Tetrahedron Lett. 1990, 31, 2849;
  • Evans, D. A.; Black, W. C.; J. Am. Chem. Soc. 1993, 115, 4497;
  • Evans, D. A.; Johnson, J. S.; J. Org. Chem. 1997, 62, 786;
  • Evans, D. A.; Wu, L. D.; Wiener, J. J. M.; Johnson, J. S.; Ripin, D. H. B.; Tedrow, J. S.; J. Org Chem 1999, 64, 6411.
  • 16. Nagao, Y.; Yagi, M.; Ikede, T.; Fujita, E.; Tetrahedron Lett. 1982, 23, 201;
  • Crimmins, M. T.; King, B. W.; Tabet, E. A.; J. Am. Chem. Soc. 1997, 119, 7883;
  • Crimmins, M. T.; King, B. W.; Tabet, E. A.; Chaudhary, K.; J. Org. Chem. 2001, 66, 894.
  • 18. David, O.; Blot, J.; Bellec, C.; Fargeau-Bellassoued, M-C.; Haviari, G.; Célérier, J-P.; Lhommet, G.; Gramain, J-C.; Gardette, D.; J. Org. Chem. 1999, 64, 3122.
  • 19. Shono, T.; Matsumura, Y.; Tsubata, K.; Org. Synth. 1985, 63, 206.
  • 20. Corre, M. L.; Toupet, L.; Delaunay, D.; J. Org. Chem. 1995, 60, 6604.
  • 21. Robins, D. J.; Sakdarat, S.; Chem. Commun. 1979, 1181
  • *
    e-mail:
  • Publication Dates

    • Publication in this collection
      16 June 2008
    • Date of issue
      2008

    History

    • Received
      04 Jan 2008
    • Accepted
      14 Mar 2008
    Sociedade Brasileira de Química Instituto de Química, Universidade Estadual de Campinas (Unicamp), CP6154, 13083-0970 - Campinas - SP - Brazil
    E-mail: quimicanova@sbq.org.br