rs7903146 mutation of Type 2 diabetes mellitus-related gene TCF7L2 is not associated with polycystic ovary syndrome in a cohort of Turkey

Taşkin, Emre; Eroğlu, Semra

doi:10.1590/1806-9282.20210509

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Revista da Associação Médica Brasileira

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Sumário

Original Article • Rev. Assoc. Med. Bras. 67 (8) • Aug 2021 • https://doi.org/10.1590/1806-9282.20210509 copy

rs7903146 mutation of Type 2 diabetes mellitus-related gene TCF7L2 is not associated with polycystic ovary syndrome in a cohort of Turkey

Authorship SCIMAGO INSTITUTIONS RANKINGS

SUMMARY

OBJECTIVE

The aim of this study was to investigate whether TCF7L2 gene mutation rs7903146 is in association with polycystic ovary syndrome (PCOS).

METHODS

A total of 44 PCOS and 48 control participants were recruited for this study. After DNA extraction from peripheral blood, quantitative PCR method was used for genotyping. With a case-control study design, two groups were compared for genotype and allele frequencies as well as clinical characteristics.

RESULTS

Mean testosterone level was significantly higher in PCOS group, whereas mean progesterone level was significantly higher in control group. In PCOS group, mean thyroid-stimulating hormone (TSH) level was significantly higher in polymorphic allele carriers. Genotype and allele frequencies were not different between groups.

CONCLUSIONS

When investigated for the first time in a population from Turkey, no association between PCOS and TCF7L2 gene rs7903146 polymorphism was detected. However, considering contradictory results of other populations and low cohort scale of this study, replication studies with greater cohorts are needed.

KEYWORDS:
PCOS; TCF7L2; Rs7903146; Polymorphism; Turkey

	Controls (n=33)	PCOS (n=48)	p	Case group	Recessive model		p	Dominant model		p
Age (years)	26.1±7.1	23.7±5.8	>0.05	Genotype	CC+CT	TT		CC	CT+TT
BMI (kg/m²)	24.9±3.5	24.1±5.4	>0.05	BMI (kg/m²)	24.3±5.6	22.5±3.3	0.45	23.5±5.9	24.5±5.03	>0.05
Insulin (pmol/L)	127±50	109±40	>0.05	Insulin (pmol/L)	107±37	99±36	0.59	108±43	105±32	>0.05
Testosterone (nmol/L)	1.09±0.39	1.58±0.57	<0.01	Testosterone (nmol/L)	1.59±0.60	1.53±0.29	0.831	1.46±0.63	1.68±0.52	>0.05
Fasting glucose (mmol/L)	4.96±0.53	5.04±0.42	>0.05	Fasting glucose (mmol/L)	5.03±0.44	5.16±0.28	0.509	4.99±0.44	5.09±0.41	>0.05
Estradiol (pmol/L)	205.94±90.31	196.03±88.84	>0.05	Estradiol (pmol/L)	204±89	132±66	0.086	197±74	195±101	>0.05
FSH (IU/L)	7.67±2.76	6.96±2.08	>0.05	FSH (IU/L)	6.81±1.96	7.91±2.81	0.227	6.90±2.22	6.98±2.02	>0.05
LH (IU/L)	8.13±3.90	8.59±4.28	>0.05	LH (IU/L)	8.67±4.36	7.93±3.89	0.749	8.86±4.41	8.33±4.24	>0.05
Progesterone (ng/mL) (nmol/L)*	15.45±12.75	3.85±7.63	<0.01	Progesterone (ng/mL) (nmol/L)*	3.65±7.70	7.55±3.38	0.408	2.05±1.36	5.29±10.0	>0.05
Prolactin (μg/L)	10.8±5.18	11.7±4.68	>0.05	Prolactin (μg/L)	11.58±4.8	12.90±3.74	0.6	12.63±4.98	11.01±4.43	>0.05
TSH (IU/L)	2.17±1.09	2.47±1.16	>0.05	TSH (IU/L)	2.59±1.13	1.74±1.10	0.095	2.02±0.99	2.80±1.18	0.028

		Controls (n=44)	PCOS (n=48)	p
rs7903146 n (%)	CC	22 (55)	21 (44)	>0.05
	CT	10 (25)	21 (44)
	TT	8 (20)	6 (12)
Allele frequencies C/T		0.67/0.33	0.66/0.34	>0.05
rs7903146 n (%)	CC	22 (55)	21 (44)	>0.05
rs7903146 n (%)	CT+TT	18 (45)	27 (56)	>0.05

		Unadjusted model		Adjusted model*
		OR (95%CI)	p	OR (95%CI)	p
Recessive model	CC+CT	1	0.3	1	0.41
Recessive model	TT	0.571 (0.180–1.812)	0.3	0.607 (0.187–1.970)	0.41
Dominant model	CC	1	0.3	1	0.19
Dominant model	CT+TT	1.571 (0.675–3.657)	0.3	1.810 (0.751–4.361)	0.19

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[1] * Corresponding author: emretaskin@gmail.com