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Isoenzymes for monitoring deterioration of Euterpe espiritosantensis Fernandes seeds

Red-palmito Euterpe espiritosantensis Fernandes seeds are recalcitrants because they exhibit germination decreases by dehydration and have short longevity. The objective of this work was to identify efficient enzymatic systems to monitoring deterioration and loss of germination capacity of red-palmito seeds. Seeds were dryed during 0, 20, and 40h (resulting moisture content of 46, 40 and 36%, respectively) and stored at 15ºC, inside closed plastic bags, during 54 weeks. The seed quality was evaluated, in six weeks intervals, by germination test and by enzymatic activity of glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, phosphoglucomutase, phosphoglucose isomerase and peroxidase, using the electrophoresis technique in polyacrylamide gel. The isoenzyme peroxidase was the unique efficient way to monitor deterioration and loss of germination capacity of red-palmito seeds.

Palm; Recalcitrant Seedseed and Peroxidase


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