ROOTING OF MINICUTTINGS OF <i>Castanea sativa</i> Mill. HYBRID CLONES

Dantas, Ângelo Kidelman; Majada, Juan; Dantas, Fernando Kidelmar; Delatorre, Carolina; Granda, Victor; Vallejo, Paula; Feito, Isabel

doi:10.1590/0100-67622016000300010

Ângelo Kidelman Dantas ^**Corresponding author.

Universidade Federal de Campina Grande, Centro de Educação e Saúde - Campus de Cuité, Cuité, PB - Brasil. E-mail: <kidelman3@hotmail.com>.

Juan Majada

Centro Tecnológico y Forestal de la Madera (CETEMAS), Área de Desarrollo Forestal Sostenible, Grado, Principado de Asturias - España. E-mail: <jmajada@cetemas.com> and <pauforestales@hotmail.com>.

Fernando Kidelmar Dantas

Universidade Federal do Sul e Sudeste do Pará, Faculdade de Engenharia de Minas e Meio Ambiente, Marabá, PA - Brasil. E-mail: <kidelmar@ufpa.br>.

Carolina Delatorre

Universidad de Oviedo, Departamiento de Biología Organismos y Sistemas, Oviedo, Principado de Asturias - Espanha. E-mail: <delatorre85@gmail.com> and <victorgrandagarcia@gmail.com>.

Victor Granda

Universidad de Oviedo, Departamiento de Biología Organismos y Sistemas, Oviedo, Principado de Asturias - Espanha. E-mail: <delatorre85@gmail.com> and <victorgrandagarcia@gmail.com>.

Paula Vallejo

Centro Tecnológico y Forestal de la Madera (CETEMAS), Área de Desarrollo Forestal Sostenible, Grado, Principado de Asturias - España. E-mail: <jmajada@cetemas.com> and <pauforestales@hotmail.com>.

Isabel Feito

Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Área de Cultivo Hortofrutícola y Forestal, Grado, Principado de Asturias - España. E-mail: <ifeito@serida.org>.

*Corresponding author.

Table 1 Survival, rooting and callus formation average of minicuttings of chestnut hybrids (C. crenata x C. sativa), on leaving the greenhouse, and final survival, on leaving the shade house, in response to IBA application with five clones.

Tratamentos		Sobrevivência	Enraizamento	Calo	Sobrevivência
AIB	mg.L-1	60 dias (%)	60 dias (%)	60 dias (%)	90 dias (%)
T1	0	96,7 a	12,5 b	62,5 a	66,7 a
T2	2500	93,3 ab	33,3 a	46,7 ab	60,0 a
T3	5000	95,8 a	38,3 a	44,2 ab	69,6 a
T4	7500	90,8 ab	39,2 a	34,2 bc	68,1 a
T5	10000	80,0 b	40,8 a	20,8 c	73,5 a
Qui quadrado		<0,0001^* ANOVA performed on data transformed using square root of x/100.	<0,0001^* ANOVA performed on data transformed using square root of x/100.	<0,0001^* ANOVA performed on data transformed using square root of x/100.	0,751 ^ns * indicates significant differences, and
Clones	C_3	94,2 a	26,7 cd	54,2 a	68,8 a
	C_7810	86,7 a	45,8 ab	26,7 b	72,7 a
	C_2671	96,7 a	52,5 a	20,8 b	74,6 a
	C_111	88,3 a	6,7 d	55,8 a	25,0 a
	C_90.025	90,8 a	32,5 bc	50,8 a	59,0 a
Qui quadrado		0,037^* ANOVA performed on data transformed using square root of x/100.	<0,0001^* ANOVA performed on data transformed using square root of x/100.	<0,0001^* ANOVA performed on data transformed using square root of x/100.	0,039^* ANOVA performed on data transformed using square root of x/100.
ANOVA	Tratamento	0,005^* ANOVA performed on data transformed using square root of x/100.	0,001^* ANOVA performed on data transformed using square root of x/100.	<0,0001^* ANOVA performed on data transformed using square root of x/100.	0,263 ^ns * indicates significant differences, and
	Clone	0,142 ^ns * indicates significant differences, and	<0,0001^* ANOVA performed on data transformed using square root of x/100.	<0,0001^* ANOVA performed on data transformed using square root of x/100.	0,391 ^ns * indicates significant differences, and
	Trat. x Clone	0,394 ^ns * indicates significant differences, and	0,010^* ANOVA performed on data transformed using square root of x/100.	0,244 ^ns * indicates significant differences, and	0,277 ^ns * indicates significant differences, and

Table 2 Rooting average of minicuttings of chestnut hybrids (C. crenata x C. sativa), on leaving the greenhouse, and final survival on leaving the shade house, in response to IBA application with five clones, comparing the treatments within each clone (column), and the clones in each treatment (row).

Tratamentos	Enraizamento médio (%)
AIB (mg.L^-1) / Clone	C_3	C_7810	C_2671	C_111	C_90.025
T1 – 0	4,2 b A	29,2 a A	16,7 b A	4,2 a A	8,3 b A
T2 – 2500	16,7 ab B	70,8 a A	45,8 ab AB	8,3 a B	25,0 b AB
T3 – 5000	29,2 ab AB	50,0 a AB	70,8 a A	8,3 a B	33,3 ab AB
T4 – 7500	29,2 ab BC	45,8 a B	83,3 a A	4,2 a C	33,3 ab BC
T5 – 10.000	54,2 a A	33,3 a A	45,8 ab A	8,3 a A	62,5 a A
Tratamentos	Sobrevivência media (%)
T1 – 0	100,0 a A	71,4 a A	50,0 a A	100,0 a A	50,0 a A
T2 – 2500	50,0 a A	64,7 a A	72,7 a A	0,0 a A	50,0 a A
T3 – 5000	42,9 a BC	91,7 a A	82,4 a AB	0,0 a C	50,0 a BC
T4 – 7500	71,4 a A	63,6 a A	80,0 a A	0,0 a A	50,0 a A
T5 – 10.000	84,6 a A	75,0 a A	63,6 a A	50,0 a A	73,3 a A

Table 3 Average number of primary roots of minicuttings of chestnut hybrids (C. crenata x C. sativa), on leaving the greenhouse, in response to IBA application to five clones

Tratamentos	Nº médio de raízes primaria						ANOVA
AIB (mg.L^-1) /Clone	C_3	C_7810	C_2671	C_111	C_90.025	Media/Trat	Clone
T1 – 0	2,00 — —	2,29 a A	2,00 a A	2,00 — —	1,50 a A	2,07 a	0,927 ns
T2 – 2500	1,50 a A	2,71 a A	2,27 a A	1,00 a A	2,00 a A	2,28 a	0,443 ns
T3 – 5000	3,29 a A	3,42 a A	2,76 a A	1,50 a A	2,25 a A	2,87 a	0,469 ns
T4 – 7500	2,29 a A	5,18 a A	3,80 a A	1,00 — —	2,00 a A	3,53 a	0,154 ns
T5 – 10.000	2,23 a A	4,75 a A	2,36 a A	2,00 a A	3,67 a A	3,10 a	0,22 ns
Media/Clone	2,38 AB	3,60 A	2,89 AB	1,50 B	2,67 AB
ANOVA
Tratamento	0,544 ns	0,177 ns	0,187 ns	0,772 ns	0,397 ns		0,478 ns
Clone							0,037 ^* * Indicates significant differences and ns indicates non-significance (P<0,05). Averages followed by the same letter do not differ according to a Tukey test (α< 0,05). For each variable, the lower case letters compare treatments for a specific clone (column), while capitals compare the different clones within each treatment (row). - indicates data that was not analysed due to there being less than two cases.
Trat x Clone							0,844 ns

Table 4 Average root morphology of minicuttings of chestnut hybrids (C. crenata x C. sativa), on leaving the greenhouse, in response to IBA application to five clones.

Tratamentos	Morfologia
AIB (mg.L^-1)	Longitud total (cm)	Área projetada (cm²)	Área superficial (cm²)	Diâmetro médio (mm)	Volume radicular (cm³)	Nº de ápices de ápices radiculares
T1_0	17,15 b	4,16 a	13,07 a	2,02 a	0,285 a	26,7 b
T2_2500	22,36 ab	4,83 a	15,16 a	2,19 a	0,335 a	40,8 ab
T3_5000	27,19 a	5,49 a	17,24 a	2,07 a	0,327 a	44,8 a
T4_7500	22,05 ab	5,25 a	16,49 a	2,32 a	0,372 a	34,0 ab
T5_10000	20,41 ab	4,18 a	13,13 a	2,01 a	0,310 a	35,7 ab
Clones_Nº
C _3	18,36 a	4,39 a	13,78 a	2,26 a	0,255 a	30,5 a
C_7810	25,97 a	5,75 a	18,08 a	2,24 a	0,365 a	40,1 a
C_2671	22,62 a	4,63 a	14,55 a	2,02 a	0,336 a	39,4 a
C_111	15,61 a	3,68 a	11,55 a	1,95 a	0,214 a	28,1 a
C_90025	22,43 a	4,58 a	14,39 a	2,06 a	0,364 a	40,3 a
ANOVA
Tratamento	0,31 ns	0,551 ns	0,551 ns	0,307 ns	0,478 ns	0,334 ns
Clone	0,053 ns	0,069 ns	0,069 ns	0,434 ns	0,103 ns	0,213 ns
Trat x Clone	0,458 ns	0,041 *	0,041 *	0,020 *	0,680 ns	0,485 ns

[1] *Corresponding author.

Brasil

Brasil

ROOTING OF MINICUTTINGS OF Castanea sativa Mill. HYBRID CLONES

ENRAIZAMENTO DE MINIESTACAS DE CLONES HÍBRIDOS DE Castanea sativa Mill

ABSTRACT