Effects of Heat Stress on the Development of GABAergic Neurons in the HPG Axis of Wenchang Chicks

Liang, W; Lu, BB; Liang, C; Yu, ZQ; Xie, XZ; Chen, Z

doi:10.1590/1806-9061-2018-0954

ABSTRACT

This study conducted an in-depth investigation on the development of GABAergic neurons and their receptors in HPG axis-related target organs of Wenchang chicks under heat stress. One-day-old healthy Wenchang chicks were randomly divided into control (CK) and heat stress (HS) groups. Chicks in the HS group were placed in a 40±0.5°C climatic chamber for HS treatment from 13:00 to 15:00 daily. By immunohistochemistry and Western blotting, GABA and GABA_A receptor (GABA_AR) expression in the hypothalamus of the HS group was significantly higher (p<0.05), but GABA_B receptor (GABA_BR) expression was significantly lower than that of the CK group (p<0.05). Expression of GABA and its two receptors in the pituitary tissues of the HS group was significantly lower than in the CK group (p<0.05). Expression of GABA and GABA_BR in ovaries in the HS group was significantly higher, but expression of GABA_AR in the testes of the HS group was lower than that of the CK group (p<0.05). In the male chicks, expression of GABA and its two receptors in the hypothalamus, pituitary, and testicular tissues of the HS group was significantly higher than that of the CK group (p<0.05). Western blotting showed that the GABA_AR and GABA_BR expression of the HS group was significantly higher than that of the CK group at 3 and 5 weeks of age. Thus, HS caused GABAergic nervous system disorder in the HPG axis of Wenchang chicks and seriously hindered the normal development of GABAergic neurons in chicks, leading to the disorder of the expression of GABA and its receptors in tissues.

Keywords:
Heat stress; GABAergic neurons; HPG axis; Wenchang chick

[1] Corresponding author e-mail address
Chen Z Hainan Normal University, Long Kun Nan Road, Haikou City, 571158, P.R. China. Phone: 0898-65883521 Email: zh.chen@hainnu.edu.cn

Item	Group	Age(week)
Item	Group	1	2	3	4	5	6
GABA_A receptor	CK	4.93±0.66^bc	5.93±1.65^b	11.74±1.41^aA	11.13±1.67^aA	4.35±1.39^bc	4.09±1.24^c
GABA_A receptor	HS	5.39±1.51^b	6.37±0.3^ab	7.42±1.8^aB	6.65±1^abB	5.62±1.8^ab	5.89±1.71^ab
GABA_B receptor	CK	3.58±1.03^dA	9.5±0.92^aA	4.53±0.41^bc	4.88±0.62^bA	4.5±0.45^bc	3.65±0.65^cd
GABA_B receptor	HS	6.01±0.57^bB	7.54±1.39^aB	4.2±0.3^c	3.62±0.78^cB	3.91±0.64^c	4.52±1.68^c
GABA	CK	1.25±0.33^d	1.03±0.13^dB	2.55±0.43^cB	4.29±0.46^b	4.01±0.4^bB	6.56±0.38^aA
GABA	HS	1.22±0.15^c	1.26±0.09^cA	4.62±0.42^bA	4.79±0.62^b	7.1±1.24^aA	4.18±0.26^bB

Item	Group	Age(week)
Item	Group	1	2	3	4	5	6
GABA_A receptor	CK	1.82±0.15^e	2.04±0.09^c	2.90±0.19^aB	2.55±0.08^bB	2.23±0.06^dB	2.49±0.13^bA
GABA_A receptor	HS	1.90±0.19^d	2.10±0.32^d	3.53±0.17^aA	3.02±0.0^bA	2.53±0.09^cA	2.08±0.06^dB
GABA_B receptor	CK	1.62±0.11^e	1.90±0.16^d	2.39±0.05^aB	2.22±0.07^bB	2.05±0.13^cB	2.28±0.08^abA
GABA_B receptor	HS	1.67±0.08^f	2.08±0.18^d	2.80±0.12^aA	2.58±0.10^bA	2.24±0.08^cA	1.88±0.08^eB
GABA	CK	1.51±0.16^e	1.82±0.19^d	2.56±0.17^aB	2.43±0.06^bB	2.30±0.07^bc	2.24±0.08^cA
GABA	HS	1.64±0.15^e	1.92±0.05^d	3.27±0.11^aA	2.80±0.14^bA	2.35±0.14^c	1.95±0.22^dB

Item	Group	Age(week)
Item	Group	1	2	3	4	5	6
GABA_A receptor	CK	53.03±8.13^fA	113.5±5.47^c	166.55±7.97^aA	153.69±6.69^bA	73.65±7.23^eA	88.16±8.76^dB
GABA_A receptor	HS	42.91±9.11^cB	104.62±5.76^b	129.29±8.91^aB	69.53±4.53^cB	55.14±4.99^dB	135.28±6.34^aA
GABA_B receptor	CK	4.55±0.25^cA	4.34±0.64^cB	10.77±1.81^bA	12.78±0.86^aA	13.44±0.86^aA	10.03±0.82^b
GABA_B receptor	HS	2.95±0.83^eB	5.43±0.57^dA	8.38±0.62^cB	11.73±0.65^aB	8.28±1.39^cB	10.35±0.64^b
GABA	CK	4.48±0.29^e	4.79±0.54^dB	14.37±1.61^aA	6.51±0.5^cB	7.19±0.64^bcA	7.98±1.14^b
GABA	HS	4.06±0.46^d	11.76±1.38^aA	11.59±0.9^aB	8.51±0.5^bA	6.24±0.78^cB	8.14±0.68^b

Item	Group	Age(week)
Item	Group	1	2	3	4	5	6
GABA_A receptor	CK	4.52±0.27^c	4.89±0.19^bcB	6.11±0.70^aB	5.38±0.35^bB	5.26±0.08^bB	5.20±0.14^bA
GABA_A receptor	HS	4.57±0.48^d	5.19±0.27^cA	7.31±0.32^aA	6.19±0.81^bA	5.82±0.48^bA	4.59±0.13^dB
GABA_B receptor	CK	2.75±0.09^eB	2.98±0.08^dB	3.88±0.17^aB	3.45±0.17^bB	3.19±0.29^c	3.19±0.14^cA
GABA_B receptor	HS	2.90±0.14^dA	3.26±0.17^cA	5.02±0.23^aA	4.19±0.16^bA	3.37±0.10^c	2.76±0.16^dB
GABA	CK	2.66±0.18^e	2.82±0.13^dB	4.23±0.14^aB	3.95±0.06^bB	3.75±0.12^cB	3.68±0.15^cA
GABA	HS	2.72±0.13^f	3.45±0.22^dA	5.33±0.49^aA	4.53±0.17^bA	4.16±0.15^cA	3.13±0.14^eB

Item	Group	Age (Week)
Item	Group	1	2	3	4	5	6
GABA_A receptor	CK	79.77±12.16^bA	141.55±8.22^aA	38.1±7.98^dB	61.23±8.79^cA	149.67±21.09^aA	46.32±6.65^dB
GABA_A receptor	HS	45.83±4.79^dB	87.56±6.27^bB	89.58±16.14^bA	49.64±9.25^dB	69.97±17.45^cB	110.14±12.13^aA
GABA_B receptor	CK	5.24±0.92^bB	4.82±0.89^b	2.06±0.87^cB	8.45±0.78^aA	4.98±0.64^bB	5.27±0.64^bB
GABA_B receptor	HS	7.92±0.94^bA	5.55±0.87^c	9.42±1.2^aA	6.21±1.01^cB	6.37±0.5^cA	6.53±0.56^cA
GABA	CK	3.94±0.73^cB	9.59±0.79^aB	10.42±0.58^aB	3.98±0.86^cB	5.89±1.1^b	5.74±0.59^bB
GABA	HS	5.28±0.43^dA	11.78±0.7^aA	11.82±1.12^aA	6.85±0.85^cA	6.79±0.47^c	9.1±0.63^bA

Brasil

Brasil

Effects of Heat Stress on the Development of GABAergic Neurons in the HPG Axis of Wenchang Chicks

ABSTRACT

Item	Group	Age (Week)
Item	Group	1	2	3	4	5	6
GABA_A receptor	CK	5.00±0.70^c	5.53±0.21^bA	6.27±0.10^aB	5.90±0.26^abB	5.77±0.26^bB	5.78±0.28^bA
GABA_A receptor	HS	4.83±0.14^e	5.21±0.14^dB	7.53±0.21^aA	6.82±0.45^bA	6.10±0.17^cA	4.80±0.28^eB
GABA_B receptor	CK	2.65±0.25^d	3.16±0.43^c	4.45±0.16^aB	3.70±0.21^bB	3.35±0.05^cB	3.80±0.12^bA
GABA_B receptor	HS	2.50±0.11^d	2.83±0.11^d	5.61±0.71^aA	4.81±0.19^bA	4.21±0.09^cA	2.53±0.10^dB
GABA	CK	4.48±0.13^c	4.72±0.14^b	5.07±0.10^aB	4.42±0.16^cB	4.47±0.15^cA	3.93±0.14^dB
GABA	HS	4.20±0.38^e	4.56±0.13^d	6.40±0.14^aA	5.25±0.18^cA	4.20±0.15^eB	5.53±0.17^bA