Effect of remote ischemic postconditioning in inflammatory changes of the lung parenchyma of rats submitted to ischemia and reperfusion

Dorsa, Rafael Cantero; Pontes, José Carlos Dorsa Vieira; Antoniolli, Andréia Conceição Brochado; Silva, Guilherme Viotto Rodrigues da; Benfatti, Ricardo Adala; Santos, Carlos Henrique Marques dos; Pontes, Elenir Rose Cury; Goldiano, José Anderson Souza

doi:10.5935/1678-9741.20150005

Brasil

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ORIGINAL ARTICLE • Rev Bras Cir Cardiovasc 30 (3) • Jul-Sep 2015 • https://doi.org/10.5935/1678-9741.20150005 linkcopy

Effect of remote ischemic postconditioning in inflammatory changes of the lung parenchyma of rats submitted to ischemia and reperfusion

Efeito do pós-condicionamento isquêmico remoto nas alterações inflamatórias do parênquima pulmonar de ratos submetidos à isquemia e reperfusão

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Objective: To assess the effects of postconditioning remote in ischemia-reperfusion injury in rat lungs.

Methods: Wistar rats (n=24) divided into 3 groups: GA (I/R) n=8, GB (R-Po) n=8, CG (control) n=8, underwent ischemia for 30 minutes artery occlusion abdominal aorta, followed by reperfusion for 60 minutes. Resected lungs and performed histological analysis and classification of morphological findings in accordance with the degree of tissue injury. Statistical analysis of the mean rating of the degree of tissue injury.

Results: GA (3.6), GB (1.3) and CG (1.0). (GA GB X P<0.05).

Conclusion: The remote postconditioning was able to minimize the inflammatory lesion of the lung parenchyma of rats undergoing ischemia and reperfusion process.

Descriptors
Ischemia; Reperfusion; Ischemic Postconditioning; Lung Injury

Abbreviations, acronyms & symbols
AMP	Adenosine monophosphate
ATP	Adenosine triphosphate
COBEA	Brazilian College of Animal Experimentation
IPoC	Ischemic postconditioning
IPreC	Ischemic preconditioning
R-PosC	Remote postconditioning
TROS	Toxic reactive oxygen species

Grade 1	Grade 2	Grade 3	Grade 4
Normal	Mild	Moderate	Severe
Normal parenchyma on optical microscopy	Focal edema in few alveolar septa, mild congestion, neutrophils in alveolar septa less than 50 per highpower field	Moderate edema in the alveolar septa or mild edema in several septa, moderate congestion, neutrophils in alveolar septa between 50 and 100 per high-power field	Severe edema in the alveolar septa or mild edema in several septa, moderate congestion, neutrophils in alveolar septa more than 100 per high-power field

Focal edema	A	B	C
1 (normal)	0	6	8
2 (slight)	0	2	0
3 (mild)	3	0	0
4 (severe)	5	0	0
Total of animals	8	8	8
Average scores ± standard deviation	^A3.6±0.5	^B1.3±0.5	^B1.0±0.0
Median of scores	4	1	1

Authors’ roles & responsibilities
RCD	Writing; bibliographic survey
JCDVP	Review
ACBA	Guidance
GVRS	Bibliographic survey
RAB	Review
CHMS	Guidance
ERCP	Statistical analysis
JASG	Experimental Surgery

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[TFN01] Note: Kruskal Wallis test followed by Student-Newman - Keuls test. Equal letters indicate statistically significant difference. Different letters indicate statistically significant difference.

[TFN02] A x B x C: P-value=<0.001

[TFN03] A x B: P-value=0.002

[TFN04] A x C: P-value=<0.001

[TFN05] B x C: P-value=0.572