<b>Micropropagation and genetic uniformity of <i>Kalanchoe daigremontiana</i> (Crassulaceae)</b>

Nascimento, Antonia M. M. do; Rego, Mailson M. do; Souza, Bruna de B.; Nascimento, Kaline da S.; Pessoa, Angela M. dos S.; Barroso, Priscila A.; Rego, Elizanilda R. do

doi:10.1590/1807-1929/agriambi.v29n2e279742

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Original Article • Rev. bras. eng. agríc. ambient. 29 (2) • Feb 2025 • https://doi.org/10.1590/1807-1929/agriambi.v29n2e279742 copy

**Micropropagation and genetic uniformity of Kalanchoe daigremontiana (Crassulaceae)** ¹ 1 Research developed at Universidade Federal da Paraíba, Centro de Ciências Agrárias, Laboratory of Biotechnology and Plant Breeding, Areia, PB, Brazil

Micropropagação e uniformidade genética de Kalanchoe daigremontiana (Crassulaceae)

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Kalanchoe daigremontiana is an ornamental species propagated exclusively asexually. However, no in vitro studies have been conducted to assess the relationship between changes in the culture environment and genetic variations of K. daigremontiana. Therefore, this study aimed to determine the optimal concentration of salts in the Murashige & Skoog (MS) medium and the optimal concentration of sucrose in the culture medium, in addition to the quality of light, in the development of in vitro plantlets from leaf explants of K. daigremontiana. Additionally, a genetic evaluation of the resulting plantlets was conducted to study phenotypic changes that could be attributed to somaclonal variation using Random Amplified Polymorphic DNA (RAPD) markers. For this purpose, leaf explants of K. daigremontiana were disinfected and inoculated in different salt concentrations (25, 50, and 100%) of MS medium without sucrose. The cultures were transferred to a growth chamber and subjected to three light conditions (white, blue, and red) with a 16-hour photoperiod, plus the absence of light, at a temperature of 27 ± 1 °C. Explants were also inoculated in 50% salt concentrations of MS medium supplemented with various concentrations of sucrose (control - without sucrose, 1.5, 3.0, 4.5, and 6.0%). K. daigremontiana leaf explants showed optimal development under white light and in any MS medium salt concentrations. A sucrose-free culture medium was viable, and no genetic variation was observed in the plantlets compared to the parent plants under the tested conditions.

Key words:
in vitro propagation; ornamental plants; RAPD markers

HIGHLIGHTS:

The different concentrations of sucrose had a similar influence on the growth of Kalanchoe daigremontiana plantlets.

The culture medium with 25% Murashige & Skoog (MS) salts also enhances the growth of K. daigremontiana plantlets.

K. daigremontiana plantlets under sucrose concentrations, MS salts, and radiation maintain the same genetic profile.

Treatments	DF	p-value
Treatments	DF	PL	NL	LL	NR	RL	NS	SL	FMW	SPAD
IC	3	0.01**	0.01**	0.01**	0.01**	0.01**	0.05*	0.63^ns	0.01**	0.01**
CM	2	0.13^ns	1.23^ns	0.06^ns	0.07^ns	0.06^ns	0.54^ns	0.06^ns	0.06^ns	2.19^ns
IC × CM	6	0.29^ns	1.99^ns	0.08^ns	0.68^ns	0.07^ns	0.05*	0.25^ns	0.07^ns	0.56^ns
CV (%)		36.09	44.54	10.94	37.66	18.49	40.90	33.17	8.27	39.01

Irradiance conditions	PL	NL	LL	NR	RL	NS	FMW	SPAD
White	1.94 a	4.09 a	1.08 a	4.15 a	1.55 a	1.92 a	1.06 a	5.03 a
Blue	1.01 b	1.14 c	0.89 b	1.07 c	0.91 c	0.94 b	0.88 b	0.97 c
Red	1.83 a	2.67 b	1.04 a	2.48 b	1.24 b	1.52 a	0.93 b	2.72 b
Dark	1.22 b	1.28 c	0.87 c	1.28 c	0.92 c	1.01 b	0.88 b	0.87 c

Irradiance conditions	Culture medium
Irradiance conditions	MS/4	MS/2	MS
White	1.53 bA	1.87 bA	2.35 aA
Blue	0.90 aB	0.87 aB	1.04 aC
Red	1.69 aA	1.24 aB	1.62 aB
Dark	0.97 aB	1.16 aB	0.90 aC

Treatments	DF	Morphological characteristics
		p-value
		PL	NL	LL	NR	RL	NS	SL	FMW	SPAD
SC	4	0.01**	0.06 ^ns	0.06^ns	0.06^ns	0.05*	0.30^ns	0.36^ns	0.40^ns	0.05*
CV (%)		30.70	40.59	27.69	38.75	39.03	51.38	52.13	46.80	54.58

Primer	Base sequence (5' - 3')	Number of amplified bands
UB- 03	GGGCGACTAC	14
UB- 04	GTGCGCAATG	11
UB- 05	TCGCATCCAG	10
UB- 06	CAGAAGCGGA	13
UB- 07	CACAGCGACA	13
UB- 08	CAAAGCGCTC	10
UB- 09	TCCCCATCAC	2
UB- 10	TGCGGGTCCT	12
UB- 11	CAGGATTCCC	10
UB- 12	GTGGAGTCAG	10
UB- 14	CAGCACTGAC	7
Mean		10.18
Total		112

Unidade Acadêmica de Engenharia Agrícola Unidade Acadêmica de Engenharia Agrícola, UFCG, Av. Aprígio Veloso 882, Bodocongó, Bloco CM, 1º andar, CEP 58429-140, Tel. +55 83 2101 1056 - Campina Grande - PB - Brazil
E-mail: revistagriambi@gmail.com

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[1] * Corresponding author - E-mail: ammarquesdo@ubu.es

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Micropropagation and genetic uniformity of Kalanchoe daigremontiana (Crassulaceae) 1 1 Research developed at Universidade Federal da Paraíba, Centro de Ciências Agrárias, Laboratory of Biotechnology and Plant Breeding, Areia, PB, Brazil

Micropropagação e uniformidade genética de Kalanchoe daigremontiana (Crassulaceae)

ABSTRACT

HIGHLIGHTS:

**Micropropagation and genetic uniformity of Kalanchoe daigremontiana (Crassulaceae)** ¹ 1 Research developed at Universidade Federal da Paraíba, Centro de Ciências Agrárias, Laboratory of Biotechnology and Plant Breeding, Areia, PB, Brazil