A taxonomic study on Venezuelan species of the genus Lespesia Robineau-Desvoidy, 1863 (Diptera, Tachinidae) is done, based on the collection of the "Museo del Instituto de Zoología Agrícola Francisco Fernández Yépez (MIZA) de la Universidad Central de Venezuela - Maracay". Six species are recognized, two of which are described as new, Lespesia giovannae sp. nov. and Lespesia oscari sp. nov., and three recorded from Venezuela for the first time: Lespesia affinis (Townsend, 1927), Lespesia protoginoi (Blanchard, 1966) and Lespesia spitzi Guimarães, 1983. Redescriptions and key to species are given.
Diptera; Lespesia; Neotropical Region; Tachinidae; Taxonomy