The objective of this work was to establish the best salt and BAP concentration in MS medium for multiplication of 'Barrier' and 'Cadaman' Prunus sp. rootstocks. Nodal segments were introduced in test tubes with 10 mL of MS medium supplemented with different salt concentrations (MS; ½MS; and 2/3MS) arranged with five BAP concentrations (0; 1.5; 2.5; 3.5; and 4.5 muM). It was applied a factorial 2x3x5, in a complete randomized block design with four replications composed by five test tubes each one. It was inoculated one nodal segment for each test tub. The evaluations was carried out after five weeks in room culture with light intensity of 20 muE m-2 s-1, photoperiod of 16 hours and temperature of 24 ± 4ºC. It was verified that cultivar 'Barrier' showed the higher average number of buds and shoots. The reduction of the salt concentration of the culture medium increased the number of shoots, although with smaller size. The polynomial regressions of the variables number of buds, shoots per explant, and length of shoots presented a quadratic behavior to BAP levels, presenting the maximum points 31.2 buds per explant; 4.6 shoots per explant; and 8.1 mm of length at BAP concentrations of 3.3; 3.1; e 3.1 muM, respectively.
6-benzilaminopurine; tissue culture; micropropagation; peach tree
