DNA content estimation of Fig and Black Mulberry using flow cytometry

Conteúdo de ADN estimado de Figueira e Amoreira-Preta utilizando citometria de fluxo

Abstract

In this study, fig and black mulberry DNA contents were estimated using DAPI fluorescence stain in flow cytometry. The 2C DNA contents of the fig and black mulberry were found as 0.82 pg and 8.34 pg, respectively. The calculated 1C value of genome size of fig is 401.8 Mbp and that of black mulberry is 4086.6 Mbp. The ratio of 2C DNA content and 1C genome of the black mulberry was 10.17 times that of the fig although fig is diploid and black mulberry is decosaploid.

Index terms
Ficus carica; Morus nigra; DAPI; DNA content; flow cytometry; genome size