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Rooting of hardwood cuttings of pear tree with IBA under B.O.D. chamber and greenhouse environment

The rooting of cuttings pear under controlled environment of chamber (B.O.D. type) and greenhouse was searched. Hardwood cuttings of 'Limeira' pear, without leaves, measuring 25 cm of length, were treated with 0, 2000, 4000 and 6000 mg.L-1 of indolbutyric acid (IBA), for 10 seconds. The vermiculite and sand mixture substrate (2:1 v/v) was used and humidified with half contends MS saline solution and sucrose 1%. The cutting stayed per 42 days inside both B.O.D. chamber (temperature of 25ºC, relative humidity of 90% and photoperiod of 8 hours) and greenhouse with irrigation for aspersion, without environmental control. In B.O.D chamber, the cuttings not treated with IBA began an intense sprouting of the buds and callus formation after seven days of planting. On the other hand in greenhouse atmosphere, those cuttings delayed 21 days for the beginning of the bud sprouting, showing smaller callus development. In the cuttings treated with IBA, the callus appeared in the areas of the cuts after the third day of incubation in the stove B.O.D. The roots were developed starting from the tissues of the base and of the callus, becoming clearer from the 14th and 28th day, respectively, for the stove atmosphere and of greenhouse. After 42 days, the best rooting percentage (83%) was verified in the treatment with IBA at 2000 mg.L-1, in B.O.D chamber atmosphere. The utilization of B.O.D chamber, with controlled temperature, light and relative humidity, showed to be more advantageous than the process of greenhouse in the rooting of woody cutting of the 'Limeira' pear tree, that could favor the system of vegetative propagation of the pear tree and to shorten the period of formation of young plants.

vegetative propagation; cutting and indolbutyric acid


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