Sucrose concentration and blueberry plant density in temporary immersion systems (TIS)

Concentração de sacarose e densidade de planta de mirtilo em sistemas de imersão temporária (TIS)

Abstract

The aim was to determine the ideal concentration of sucrose along with the number of plants per container in the face of micropropagation of blueberry under TIS. In the experiment conducted with sucrose concentration (0, 10, 20, e 30 g L-1), we utilized a WPM medium, taking into account 30 explants per container. For the experiment dealing with blueberry plant density, the same medium with 20 g L-1 of sucrose in conjunction with 30, 40, 50 and 60 explants per container was used. After 90 days from the installation of the trial, the following response variables were assessed herein: total length of the plant, number of shoots, length of the largest shoot, number of hyperhydric shoots, length of hyperhydric shoots, and number of healthy leaves. Under a sucrose concentration corresponding to 20 g L-1 the best possible results were obtained. Survival rates of 22 and 80% under the influence of both 10 and 20 g L-1 treatments were reported, pointing out that plants exposed to 20 g L-1 treatment were more amenable to acclimatization. Thus, for the micropropagation of blueberry under TIS the use of 20 g L-1 sucrose and 60 plants/container comes to being highly recommended to increase yield.

Index terms
Vaccinium ashei Reade; Bioreactor; Micropropagation