The cultivar 'IAC Gomo-de-Mel'is an economically important species due to different sensory characteristics. One of the techniques to obtain higher number of plants is micropropagation. Thus, the aim of this study was to evaluate the effects of TIBA (2,3,5 - triiodobenzoic acid) in the formation of shoots and roots of pineapple 'IAC Gomo-de-Mel ', as well as possible changes in the morphology of leaf tissues. It were used segments of the gems in the crown of pineapple, with approximately 1 cm, arising from cultivation in liquid medium supplemented with 1.0 mg L-1 BAP+ 0.5 mg L-1 NAA , and subsequently inoculated in MS liquid containing various concentrations of TIBA in light condition and controlled temperature, deploying three different experiments for biometric, biochemical and anatomical analyzes. The experiments were implanted in a completely randomized experimental design with 8 treatments and 4 replicates for biometric analysis and 5 replicates for biochemical and anatomical analysis. At the end of 60 days, were evaluated the number and length of shoots, length of the mother plant and the number of roots. The analysis of the activity of IAA-oxidase was performed on the day of the experiment and at 15, 30, 45 and 60 days of cultive. We conclude that the TIBA alone does not induce shoots formation. The reduction in the IAA-oxidase activity was related to the emission of new roots and an increase in leaf thickness occurred after exogenous application of TIBA in pineapple 'Gomo-de-Mel' cultivated in vitro.
pineapple-of-gomo; IAA oxidase; micropropagation; plant anatomy
