This study aimed to evaluate morphogenetic responses of different cupuassu explants (Theobroma grandiflorum Schum.) under diverse conditions of in vitro culture. 2,4-D (4,52; 6,78 and 9,05 µM) promoted the formation of friable calluses in embryonic axes and TDZ (2,0 and 3,0 µM) induced a high frequency of friable calluses and smaller oxidation in segments of young leaves. 2,4-D combined with KIN promoted the initiation of friable calluses while NAA combined with KIN promoted the rhizogenesis in cotyledon segments. In the presence of 2,26 µM 2,4-D and 13,94 µM KIN it was noticed the growth of friable calluses in stem segments. The addition of coconut water, 2iP and NAA stimulated the fast growth of calluses in embryonic axis, stem segments, and chlorophyllous young leaf segments.
Theobroma grandiflorum; calli; growth regulators
