BLOOD INSULIN LEVEL BIOSENSOR FOR ATHLETES

BIOSSENSOR PARA NÍVEL DE INSULINA SANGUÍNEO EM ATLETAS

BIOSENSOR PARA EL NIVEL DE INSULINA EN SANGRE DE DEPORTISTAS

ABSTRACT

Introduction:

The pancreas releases insulin to assist the human body in utilizing blood glucose. It regulates metabolism by promoting the absorption of glucose into the blood.

Objective:

This work aimed to create an electrochemical biosensor based on magnetic graphene nanomaterial to measure insulin levels in athletes’ blood. Method: A magnetic graphene nanocomposite created by graphene oxide (GO) and Fe-Ni bimetallic oxides on a glassy carbon electrode was synthesized using the electrochemical deposition method (GCE).

Results:

The immediate electrical deposition of Fe-Ni bimetallic oxide nanoparticles with the spherical shape on the GO nanosheet without aggregations was validated by structural characterizations of Fe-Ni/GO/GCE using XRD and SEM. The electrochemical results for insulin determination showed good sensitivity and anti-interference capability. The applicability and accuracy of the proposed electrochemical sensor to detect insulin were explored by blood serum samples from sportsmen.

Conclusion:

The results assigned acceptable RSD values (3.31% to 4.30%) and confirmed the feasibility of the proposed sensor for detecting athletes’ blood insulin. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

Keywords:
Biosensing Techniques; Nanoparticles; Insulin; Doping in Sports