Influence of mononuclear cell therapy on disc degeneration in rabbits

Fávaro, Rodrigo Caldonazzo; Arruda, André de Oliveira; Vialle, Luiz Roberto Gomes; Vialle, Emiliano Neves

doi:10.1016/j.rboe.2016.10.003

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ORIGINAL ARTICLES • Rev. bras. ortop. 51 (06) • Nov-Dec 2016 • https://doi.org/10.1016/j.rboe.2016.10.003 copy

Influence of mononuclear cell therapy on disc degeneration in rabbits^{^☆} ☆ Study conducted at the Service of Orthopedy and Traumatology, Hospital Universitário Cajuru, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

OBJECTIVE:

The objective of this research was to evaluate the influence of autologous mononuclear stem cells injections on histological changes of collagen in the fibrous annulus of the intervertebral disk after experimental injury.

METHODS:

32 New Zealand rabbits were submitted to intervertebral disk puncture, followed by intradiscal injection of mononuclear cells from the iliac crest versus saline injection in the following time periods: two months after the injury (SC2M and SS2M), two weeks (SC2W and SS2W) immediately after injury (SCCP and SSCP), and without inducing degeneration (SCSP and SSSP). Two months after cell therapy, the animals were euthanized and collagen changes in the intervertebral discs were histologically evaluated.

RESULTS:

There were significant differences in ELAF between SS2W and SS2S groups (p = 0.018). This difference was due to an increase in type I collagen in SS2W group (56.7%) compared to SC2S (13.28%).

CONCLUSION:

Treatment with mononuclear mesenchymal stem cells reduced changes in the type I and III collagen distribution in rabbits AF degenerated discs up to two weeks after the induction of degeneration.

Keywords:
Collagen; Intervertebral disk; Cell and tissue-based therapy; Histology; Rabbit

Group	Stem cells
	SC2M	SC2W	SCCP	SCSP
Rabbits (n= 14)	4	3	4	3
Discs – intervention (n= 37)	12	9	7	9
Discs – control (n= 60)	16	14	16	14

Group	Saline
	SS2M	SS2W	SSCP	SSSP
Rabbits (n= 12)	3	2	4	3
Discs – intervention (n= 35)	9	6	12	8
Discs – control (n= 55)	15	10	17	13

Variable	Col	Group	n	Mean	Minimum	Maximum	Standard deviation	p-Value^a
ILAF	I	SC2M	12	73.37	2.39	98.65	29.18	0.286
	I	SS2M	9	85.5	42.5	97.5	18.2	0.286
	III	SC2M	12	26.63	1.35	97.61	29.18	0.286
	III	SS2M	9	14.5	2.5	57.5	18.2	0.286
	I	SC2W	9	93.36	88.93	98.33	3.28	0.157
	I	SS2W	6	95.6	87.7	99.6	4.2	0.157
	III	SC2W	9	6.64	1.67	11.7	3.28	0.157
	III	SS2W	6	4.4	0.4	12.3	4.2	0.157
	I	SCCP	7	96.61	92.56	99.56	2.40	0.800
	I	SSCP	12	96.9	90.4	99.4	2.8	0.800
	III	SCCP	7	3.39	0.44	7.44	2.40	0.800
	III	SSCP	12	3.1	0.6	9.6	2.8	0.800
	I	SCSP	9	91.16	78.9	98.52	6.7	0.149
	I	SSSP	8	94.8	85.9	98.7	4.4	0.149
	III	SCSP	9	8.84	1.48	21.91	6.7	0.149
	III	SSSP	8	5.2	1.3	14.1	4.4	0.149

Variable	Col	Group	n	Mean	Minimum	Maximum	Standard deviation	p-Value^a
ELAF	I	SC2M	12	25.85	1.24	68.87	19.58	0.201
	I	SS2M	9	39.2	13.1	82.8	21.0	0.201
	III	SC2M	12	74.15	31.13	98.76	19.58	0.201
	III	SS2M	9	60.8	17.2	86.9	21.0	0.201
	I	SC2W	9	13.28	2.72	20.99	6.55	0.18
	I	SS2W	6	56.7	8.7	98.7	32.8	0.18
	III	SC2W	9	86.72	79.1	97.28	6.55	0.18
	III	SS2W	6	43.3	1.3	91.3	32.8	0.18
	I	SCCP	7	14.91	6.38	29.11	8.16	0.63
	I	SSCP	12	9.1	1.6	21.9	6.9	0.63
	III	SCCP	7	85.9	70.89	93.62	8.16	0.63
	III	SSCP	12	90.9	78.1	98.5	6.9	0.63
	I	SCSP	9	12.5	2.29	36.52	10.54	0.773
	I	SSSP	8	15.0	3.1	60.6	19.7	0.773
	III	SCSP	9	87.95	63.48	97.71	10.54	0.773
	III	SSSP	8	85.0	39.4	96.9	19.7	0.773

Variable	Col	Group	n	Mean	Minimum	Maximum	Standard deviation	p-Value^a
Entire AF	I	SC2M	12	49.61	2.16	78.59	22.21	0.155
	I	SS2M	9	62.3	27.8	88.9	16.1	0.155
	III	SC2M	12	50.39	21.41	97.84	22.21	0.155
	III	SS2M	9	37.7	11.1	72.2	16.1	0.155
	I	SC2W	9	53.32	47.90	59.15	3.62	0.25
	I	SS2W	6	76.1	48.2	97.4	17.5	0.25
	III	SC2W	9	46,68	40.85	52.10	3.62	0.25
	III	SS2W	6	23.9	2.6	51.8	17.5	0.25
	I	SCCP	7	55.76	51.10	64.33	4.57	0.151
	I	SSCP	12	53.0	48.6	60.7	3.5	0.151
	III	SCCP	7	44.24	35.67	48.90	4.57	0.151
	III	SSCP	12	47.0	39.3	51.4	3.5	0.151
	I	SCSP	9	51.61	43.83	64.43	6.59	0.534
	I	SSSP	8	54.9	45.5	79.5	11.1	0.534
	III	SCSP	9	48.39	35.57	56.17	6.59	0.564
	III	SSSP	8	45.1	20.5	54.5	11.1	0.564

Sociedade Brasileira de Ortopedia e Traumatologia Al. Lorena, 427 14º andar, 01424-000 São Paulo - SP - Brasil, Tel.: 55 11 2137-5400 - São Paulo - SP - Brazil
E-mail: rbo@sbot.org.br

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[1] * Corresponding author. E-mail: evialle@hotmail.com (E.N. Vialle).

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Influence of mononuclear cell therapy on disc degeneration in rabbits☆ ☆ Study conducted at the Service of Orthopedy and Traumatology, Hospital Universitário Cajuru, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.

ABSTRACT

OBJECTIVE:

METHODS:

RESULTS:

CONCLUSION:

Influence of mononuclear cell therapy on disc degeneration in rabbits^{^☆} ☆ Study conducted at the Service of Orthopedy and Traumatology, Hospital Universitário Cajuru, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.