Open-access In vitro establishment of Brazilian pepper (Schinus terebinthifolius Raddi) at different concentrations of 6-benzylaminopurine

Brazilian pepper (Schinus terebinthifolius Raddi) is Anacardiaceae presenting medicinal proprieties which are extensively exploited by common citizens to treat uterine inflammation, especially in the northeast region of Brazil. The aim of this study was to establish ideal concentrations of 6-benzilaminopurine (BAP) for the in vitro establishment of Schinus terebinthifolius using nodal, internodal, cotyledonary and apical stem segments. Explants were cultured on MS medium supplemented with 3% sucrose, 0.1 g L-1 myo-inositol and distinct concentrations of BAP: 0, 2.25, 4.5, 9.0 and 18.0 μM. The evaluated parameters were sprout induction, and sprout number and length. Nodal segment is the most suitable explant for the in vitro establishment of Brazilian pepper. Internodal segments only develop callus, not inducing adventitious sprouts. The treatment with 4.5 μM BAP was the most responsive to Schinus terebinthifolius regeneration.

Schinus terebinthifolius; medicinal plants; micropropagation


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