Optimization of protease production by the fungus<i>Monacrosporium thaumasium</i> and its action against<i>Angiostrongylus vasorum</i> larvae

Soares, Filippe Elias de Freitas; Braga, Fabio Ribeiro; Araújo, Jackson Victor de; Lima, Walter dos Santos; Mozzer, Lanuze Rose; Queiroz, José Humberto de

doi:10.1590/S1984-29612013000200048

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Research Note • Rev. Bras. Parasitol. Vet. 22 (2) • Apr-June 2013 • https://doi.org/10.1590/S1984-29612013000200048 copy

Optimization of protease production by the fungusMonacrosporium thaumasium and its action againstAngiostrongylus vasorum larvae

Otimização da produção de protease pelo fungoMonacrosporium thaumasium e sua ação contra larvas deAngiostrongylus vasorum

Authorship SCIMAGO INSTITUTIONS RANKINGS

The objectives of this study were to optimize protease production from the nematophagous fungus Monacrosporium thaumasium (NF34a) and evaluate its larvicidal activity and biological stability. An isolate of the nematophagous fungus Monacrosporium thaumasium (NF34a) was used to produce the enzyme. The Plackett-Burman design was used in order to scan which components of the culture medium could have a significant influence on protease production by the fungus NF34a. An in vitro assay was also performed to evaluate the larvicidal activity of NF34a. It was observed that only one component of the culture medium (yeast extract), at the levels studied, had any significant effect (p < 0.05) on protease production. There was a reduction (p < 0.01) in the mean number of larvae recovered from the treated groups, compared with the control groups. The results confirm previous reports on the efficiency of nematophagous fungi for controlling nematode larvae that are potentially zoonotic. Thus, given the importance of biological control, we suggest that further studies should be conducted on the protease produced by the fungus Monacrosporium thaumasium.

Nematophagous fungi; Monacrosporium thaumasium; protease; Angiostrongylus vasorum

Run	Glucose (g/L)	Yeast extract (g/L)	K₂HPO₄ (g/L)	MgSO₄ (g/L)	ZnSO₄ (g/L)	FeSO₄ (g/L)	CuSO₄ (g/L)	pH	Activity (U/mL)
1	15	5	7.5	0.1	2.5 × 10⁻³	5 × 10⁻³	5 × 10⁻³	9	0
2	15	15	2.5	0.3	2.5 × 10⁻³	5 × 10⁻³	1.25 × 10⁻³	9	0
3	5	15	7.5	0.1	7.5 × 10⁻³	5 × 10⁻³	1.25 × 10⁻³	5	0
4	15	5	7.5	0.3	2.5 × 10⁻³	2 × 10⁻³	1.25 × 10⁻³	5	31.89
5	15	15	2.5	0.3	7.5 × 10⁻³	5 × 10⁻³	5 × 10⁻³	5	14.30
6	15	15	7.5	0.1	7.5 × 10⁻³	2 × 10⁻³	1.25 × 10⁻³	9	3.616
7	5	15	7.5	0.3	2.5 × 10⁻³	2 × 10⁻³	5 × 10⁻³	5	0
8	5	5	7.5	0.3	7.5 × 10⁻³	5 × 10⁻³	5 × 10⁻³	9	17.26
9	5	5	2.5	0.3	7.5 × 10⁻³	2 × 10⁻³	1.25 × 10⁻³	9	21.53
10	15	5	2.5	0.1	7.5 × 10⁻³	2 × 10⁻³	5 × 10⁻³	5	38.96
11	5	15	2.5	0.1	2.5 × 10⁻³	2 × 10⁻³	5 × 10⁻³	9	0
12	5	5	2.5	0.1	2.5 × 10⁻³	5 × 10⁻³	1.25 × 10⁻³	5	24.82

	Effect	Coeff	SE Coeff	t-test	P>\|t\|
Intercept		12.699	2.064	6.15	0.009
Glucose (g/L)	4.192	2.096	2.064	1.02	0.385
Casein (g/L)	−19.425	−9.712	2.064	−4.71	0.018
K₂HPO₄ (g/L)	−7.808	−3.904	2.064	−1.89	0.155
MgSO₄ (g/L)	2.932	1.466	2.064	0.71	0.529
ZnSO₄ (g/L)	6.493	3.247	2.064	1.57	0.214
FeSO₄ (g/L)	6.603	3.301	2.064	1.60	0.208
CuSO₄ (g/L)	−1.890	−0.945	2.064	−0.46	0.678
pH	−11.260	−5.630	2.064	−2.73	0.072

Colégio Brasileiro de Parasitologia Veterinária FCAV/UNESP - Departamento de Patologia Veterinária, Via de acesso Prof. Paulo Donato Castellane s/n, Zona Rural, , 14884-900 Jaboticabal - SP, Brasil, Fone: (16) 3209-7100 RAMAL 7934 - Jaboticabal - SP - Brazil
E-mail: cbpv_rbpv.fcav@unesp.br

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[1] *Corresponding author: Filippe Elias Freitas Soares, Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa - UFV, Av. Ph Rolfes, s/n, CEP 36570-000, Viçosa, MG, Brasil, e-mail: filippeufv@yahoo.com.br