Molecular and morphological evidence of Taenia omissa in pumas (Puma concolor) in the Peruvian Highlands

Evidência molecular e morfológica de Taenia omissa em onça-pardas (Puma concolor) dos Andes Peruanos

Abstract

A total of 41 cestodes were collected during necropsy examination on 2 pumas (Puma concolor) that were found in 2 communities in Canchis province, Cuzco region, Peru, at 4500 meters above sea level (Peruvian Andes). The cestodes were evaluated morphologically and molecularly. A fragment of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) was used as a genetic marker. All the cestodes were identified as Taenia omissa. In the present report, we give a brief description by molecular and morphological diagnosis of the cestodes and compare nucleotide sequences with previous isolates from GenBank. Upon comparison, the sequences showed a difference in the cox1 gene of 5.1 to 5.3% with other teniids sequences. This finding constitutes the first report of T. omissa in Peru and expands the geographic distribution of this parasite.

Keywords:
Taenia omissa; cestode; taeniid; puma; Puma concolor; cytochrome C oxidase subunit 1 gene