Figure 1
(a-d) Photographs showing encapsulated blastocysts of trypanorhynch metacestodes (arrows) in the peritoneal cavity of: (a) Seriola dumerili; (b) Pseudocarans dentex; c. Epinephelus haifensis; (d) Mycteroperca rubra; (e, f) Isolated blastocysts; (g) Generated post larvae (plerocerci), Bars: a-d 1 cm; e-f 0.5 cm.
Figure 2
Photomicrographs of trypanorhynch metacestodes, carmine stained isolated from the examined fish showing: (a-e) Callitetrarhynchus gracilis, (a) Entire worm, lateral view, PB pars bothridialis, PV pars vaginalis, PBL pars bulbulosa, PPB pars post bulbulosa, BU bulbs, Bar 500 µm; (b, c) The anterior part, BO bothridia, TS tentacle sheaths, TE tentacles, Bar 200 µm; (d) Tentacle (TE) and hooks (HO), Bar 40 µm; (e) Four bulbs (BU), Bar 200 µm; (f-j). Callitetrarhynchus speciosus, (f) Entire worm, lateral view, Bar 500 µm; (g, h) the anterior part, BO bothridia, TS tentacle sheaths, TE tentacles, Bar 200 µm; (i) Four bulbs, Bar 200 µm; (j) Tentacles (TE) and hooks (HO) Bar 20 µm.
Figure 4
Scanning electron micrographs of a plerocercoid of: (a, b) Callitetrarhynchus gracilis, (c, d) Callitetrarhynchus speciosus, (a) Pars bothridialis of C. gracilis, BO bothridia, TE tentacles, Bar 200 µm; (b) Enlarged tentacle (TE) with hooks (HO), Bar 50 µm; (c) Pars bothridialis of C. speciosus, BO bothridia, TE tentacles, Bar 200 µm; (d) Enlarged of tentacle (TE) with hooks (HO), Bar 20 µm.
Figure 3
Photomicrographs of trypanorhynch metacestodes, carmine stained isolated from the examined fish showing: (a-c) Protogrillotia zerbiae, (a) Entire worm, lateral view, PB pars bothridialis, PV pars vaginalis, PBL pars bulbulosa, PPB pars post bulbulosa, BU bulbs, Bar 200 µm; (b) The anterior part, BO bothridia, Bar 100 µm; (c) Four bulbs (BU), Bar 100 µm; (d-g). Grillotia brayi, (d) Entire worm, lateral view, Bar 200 µm; (e, f) The anterior part, BO bothridia, TS tentacle sheaths, TE tentacles, Bar 100 µm; (g) Four bulbs, Bar 100 µm.
Figure 5
Line diagrams of trypanorhynch metacestodes isolated in the present study: Callitetrarhynchus gracilis, (a) Entire worm, Bar 500 µm; (b) Enlarged tentacle, Bar 20 µm; Callitetrarhynchus speciosus, (c) Entire worm, Bar 500 µm; (d) Enlarged tentacle, Bar 20 µm; Protogrillotia zerbiae, (e) Entire worm, Bar 200 µm; (f) Enlarged tentacle, Grillotia brayi, (g) Entire worm, Bar 200 µm; (h) Enlarged tentacle, Bar 50 µm. PB pars bothridialis, PV pars vaginalis, PBL pars bulbulosa, PPB pars post bulbulosa, BO bothridia, TS tentacle sheaths, TE tentacles, HO hooks, BU bulbs.
Figure 6
Phylogenetic analysis and evolutionary history using the Maximum Likelihood method and Tamura 3-parameter model according to the parasites 18s rRNA sequence analysis, the percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree (s) for the heuristic search was obtained by applying the BioNJ method to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 69 nucleotide sequences. There were a total of 1094 positions in the final dataset.
Figure 2
Photomicrographs of trypanorhynch metacestodes, carmine stained isolated from the examined fish showing: (a-e) Callitetrarhynchus gracilis, (a) Entire worm, lateral view, PB pars bothridialis, PV pars vaginalis, PBL pars bulbulosa, PPB pars post bulbulosa, BU bulbs, Bar 500 µm; (b, c) The anterior part, BO bothridia, TS tentacle sheaths, TE tentacles, Bar 200 µm; (d) Tentacle (TE) and hooks (HO), Bar 40 µm; (e) Four bulbs (BU), Bar 200 µm; (f-j). Callitetrarhynchus speciosus, (f) Entire worm, lateral view, Bar 500 µm; (g, h) the anterior part, BO bothridia, TS tentacle sheaths, TE tentacles, Bar 200 µm; (i) Four bulbs, Bar 200 µm; (j) Tentacles (TE) and hooks (HO) Bar 20 µm.
Figure 4
Scanning electron micrographs of a plerocercoid of: (a, b) Callitetrarhynchus gracilis, (c, d) Callitetrarhynchus speciosus, (a) Pars bothridialis of C. gracilis, BO bothridia, TE tentacles, Bar 200 µm; (b) Enlarged tentacle (TE) with hooks (HO), Bar 50 µm; (c) Pars bothridialis of C. speciosus, BO bothridia, TE tentacles, Bar 200 µm; (d) Enlarged of tentacle (TE) with hooks (HO), Bar 20 µm.
Figure 3
Photomicrographs of trypanorhynch metacestodes, carmine stained isolated from the examined fish showing: (a-c) Protogrillotia zerbiae, (a) Entire worm, lateral view, PB pars bothridialis, PV pars vaginalis, PBL pars bulbulosa, PPB pars post bulbulosa, BU bulbs, Bar 200 µm; (b) The anterior part, BO bothridia, Bar 100 µm; (c) Four bulbs (BU), Bar 100 µm; (d-g). Grillotia brayi, (d) Entire worm, lateral view, Bar 200 µm; (e, f) The anterior part, BO bothridia, TS tentacle sheaths, TE tentacles, Bar 100 µm; (g) Four bulbs, Bar 100 µm.
Figure 5
Line diagrams of trypanorhynch metacestodes isolated in the present study: Callitetrarhynchus gracilis, (a) Entire worm, Bar 500 µm; (b) Enlarged tentacle, Bar 20 µm; Callitetrarhynchus speciosus, (c) Entire worm, Bar 500 µm; (d) Enlarged tentacle, Bar 20 µm; Protogrillotia zerbiae, (e) Entire worm, Bar 200 µm; (f) Enlarged tentacle, Grillotia brayi, (g) Entire worm, Bar 200 µm; (h) Enlarged tentacle, Bar 50 µm. PB pars bothridialis, PV pars vaginalis, PBL pars bulbulosa, PPB pars post bulbulosa, BO bothridia, TS tentacle sheaths, TE tentacles, HO hooks, BU bulbs.
Figure 6
Phylogenetic analysis and evolutionary history using the Maximum Likelihood method and Tamura 3-parameter model according to the parasites 18s rRNA sequence analysis, the percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree (s) for the heuristic search was obtained by applying the BioNJ method to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 69 nucleotide sequences. There were a total of 1094 positions in the final dataset.