ESAG6/7 |
F: ACATTCCAGCAGGAGTTGGAG |
Holland et al. (2001)Holland WG, Claes F, My LN, Thanh NG, Tam PT, Verloo D, et al. A comparative evaluation of parasitological tests and a PCR for Trypanosoma evansi diagnosis in experimentally infected water buffaloes. Vet Parasitol 2001; 97(1): 23-33. http://doi.org/10.1016/S0304-4017(01)00381-8. PMid:11337124. http://doi.org/10.1016/S0304-4017(01)003...
|
1’ at 94oC, 35 cycles: |
R: CACGTGAATCCTCAATTTTGT |
[1’ at 94oC, 2’ at 55oC, 2’ at 72oC], and 10’ at 72oC |
MINI |
F: CAACGACAAAGAGTCAGT |
Artama et al. (1992)Artama WT, Agey MW, Donelson JE. DNA comparisons of Trypanosoma evansi (Indonesia) and Trypanosoma brucei spp. Parasitology 1992; 104(Pt 1): 67-74. http://doi.org/10.1017/S0031182000060819. PMid:1319564. http://doi.org/10.1017/S0031182000060819...
|
1’ at 94oC, 35 cycles: |
R: ACGTGTTTTGTGTATGGT |
[1’ at 94oC, 2’ at 55oC, 2’ at 72oC], and 10’ at 72 oC |
MAXI |
F: TGGGTTTATATCAGGTTCATTTATG |
Li et al. (2007)Li FJ, Gasser RB, Lai DH, Claes F, Zhu XQ, Lun ZR. PCR approach for the detection of Trypanosoma brucei and T. equiperdum and their differentiation from T. evansi based on maxicircle kinetoplast DNA. Mol Cell Probes 2007; 21(1): 1-7. http://doi.org/10.1016/j.mcp.2006.03.009. PMid:16806809. http://doi.org/10.1016/j.mcp.2006.03.009...
|
1’ at 94oC, 35 cycles: |
R: CCTAATAATCTCATCCGCAGTACG |
[1’ at 94oC, 1’ at 55oC, 2’ at 72oC], and 10’ at 72oC |
RoTat 1.2-a |
F: GCGGGGTGTTTAAAGCAATA |
Claes et al. (2004)Claes F, Radwanska M, Urakawa T, Majiwa PAO, Goddeeris B, Büscher P. Variable surface glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections. Kinetoplastid Biol Dis 2004; 3(1): 3. http://doi.org/10.1186/1475-9292-3-3. PMid:15377385. http://doi.org/10.1186/1475-9292-3-3...
; |
4’ at 94oC, 35 cycles: |
R: ATTAGTGCTGCGTGTGTTCG |
WOAH (2021)World Organization of Animal Health - WOAH. Surra in all species (Trypanosoma evansi/infection). In: World Organization of Animal Health - WOAH. OIE Terrestrial Manual 2021. France: WOAH; 2021 [cited 2024 Apr 24]. p. 1-17. Available from: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.01.21_SURRA_TRYPANO.pdf https://www.woah.org/fileadmin/Home/eng/...
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[1’ at 94oC, 1’ at 59oC, 1’ at 72oC], and 5’ at 72oC |
RoTat 1.2-b |
F: GCCACCACGGCGAAAGAC |
Urakawa et al. (2001)Urakawa T, Verloo D, Moens L, Büscher P, Majiwa PA. Trypanosoma evansi: cloning and expression in Spodoptera frugiperda [correction of fugiperda] insect cells of the diagnostic antigen RoTat1.2. Exp Parasitol 2001; 99(4): 181-189. http://doi.org/10.1006/expr.2001.4670. PMid:11888244. http://doi.org/10.1006/expr.2001.4670...
; |
1’ at 94oC, 35 cycles: |
R: TAATCAGTGTGGTGTGC |
Birhanu et al. (2016)Birhanu H, Gebrehiwot T, Goddeeris BM, Büscher P, Reet NV. New Trypanosoma evansi type B isolates from Ethiopian dromedary camels. PLoS Negl Trop Dis 2016; 10(4): e0004556. http://doi.org/10.1371/journal.pntd.0004556. PMid:27035661. http://doi.org/10.1371/journal.pntd.0004...
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[1’ at 94oC, 1’ at 52oC, 1’ at 72oC], and 5’ at 72oC |
RoTat 1.2-c |
F: CTGAAGAGGTTGGAAATGGAGAAG |
Konnai et al. (2009)Konnai S, Mekata H, Mingala CN, Abes NS, Gutierrez CA, Herrera JRV, et al. Development and application of a quantitative real-time PCR for the diagnosis of Surra in water buffaloes. Infect Genet Evol 2009; 9(4): 449-452. http://doi.org/10.1016/j.meegid.2009.01.006. PMid:19460309. http://doi.org/10.1016/j.meegid.2009.01....
; |
1’ at 94oC, 35 cycles: |
R: GTTTCGGTGGTTCTGTTGTTGTTA |
Salim et al. (2011)Salim B, Bakheit MA, Kamau J, Nakamura I, Sugimoto C. Molecular epidemiology of camel trypanosomiasis based on ITS1 rDNA and RoTat 1.2 VSG gene in the Sudan. Parasit Vectors 2011; 4(1): 31. http://doi.org/10.1186/1756-3305-4-31. PMid:21375725. http://doi.org/10.1186/1756-3305-4-31...
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[1’ at 94oC, 1’ at 59oC, 1’ at 72oC], and 5’ at 72oC |
EVAB |
F: CACAGTCCGAGAGATAGAG |
Njiru et al. (2006)Njiru ZK, Constantine CC, Masiga DK, Reid SA, Thompson RCA, Gibson WC. Characterization of Trypanosoma evansi type B. Infect Genet Evol 2006; 6(4): 292-300. http://doi.org/10.1016/j.meegid.2005.08.002. PMid:16157514. http://doi.org/10.1016/j.meegid.2005.08....
|
5’ at 95oC, 30 cycles: |
R: CTGTACTCTACATCTACCTC |
|
[1’ at 94oC, 1’ at 60oC, 1’ at 72oC], and 10’ at 72oC |