Occurrence of abortions induced by <i>Neospora caninum</i> in dairy cattle from Santa Catarina, southern Brazil

Macedo, Cesar Augusto Barbosa de; Macedo, Madlaine Frigo Silveira Barbosa de; Miura, Ana Carolina; Taroda, Alessandra; Cardim, Sergio Tosi; Innes, Elisabeth Ann; Katzer, Frank; Cantón, German Jose; Chianini, Francesca; Headley, Selwyn Arlington; Garcia, João Luis

doi:10.1590/S1984-29612017051

home Sumário navigate_before Anterior Atual Seguinte navigate_next

home Sumário

Original Article • Rev. Bras. Parasitol. Vet. 26 (3) • Jul-Sep 2017 • https://doi.org/10.1590/S1984-29612017051 linkcopy

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Ocorrência de abortamentos induzidos por Neospora caninum em vacas de leite de Santa Catarina, sul do Brasil

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.

Keywords: Neosporosis; fetuses; lesion; serology; cattle

Cow Nº	Breed	Age of cattle (months)	ELISA ¹ 1 S/P from ELISA (Idexx) S/P ≥ 0.50 (positive) and <0.50 (negative).		Age of fetus (months)	Fetuses PCR	Principal histopathological findings of the brain		Fetuses IHC
Cow Nº	Breed	Age of cattle (months)	Cows	Aborted fetuses		Pool of organs	Endothelial hypertrophy	Congestion
1	JER	> 48	2.23 / 1.53	ND	Mum² 2 Mummified fetuses (mum) did not have their tissues and blood tested, and age determined.	ND	ND		ND
2	HOL	> 48	0.4	ND	7	-	0	-	-
3	JER	36	0.27	ND	8	ND	ND	ND	ND
4	HOL	33	0.12 / 0.09	ND	9	-	0	0	-
5	HOL	> 48	2.52 / 2.01	ND	5	+	0	+1	+1
6	JER	> 48	0.97 / 1.05	ND	6	-	0	+2	+2
7	HOL	24	0.0 / 0.07	0.01	8	+	+	N	-
8	HOL	25	0.13 / 0.23	0.01	8	+	+	+1	+1
9	JER	36	0.22 / 0.14	0.0	7	-	++	0	-
10	HOL	> 48	2.07 / 0.18	ND	mum	ND	ND		ND
11	JER	24	0.01	0.0	7	-	++	0	-
12	HOL	> 48	2.11 / 2.07	0.31	8	+	+	0	-
13	JER	> 48	1.9 / 2.15	3.11	7	-	++	+1	+1
14	HOL	> 48	2.27 / 2.2	0.0	7	+	+	0	-
15	JER	> 48	2.3 / 1.96	1.91	7	+	+	0	-
16	JER	> 48	2.05 / 1.09	0.0	6	-	0	+2	+2
17	JER	> 48	0.2 / 0.3	0.0	7	-	+	0	-
18	HOL	24	0.1 / 0.12	0.0	7	-	0	+2	+2
19	JER	> 48	2.01 / 1.95	3.02	7	+	+++	+2	+2
20	JER	> 48	0.29	0.0	8	-	+	0	-
21	JER	48	0.2 / 0.15	0.0	9	-	++	0	-
22	JER	>48	1.92	ND	4	ND	ND		ND
23	JER	>48	0.36 / 0.19	0.0	6	+	0	0	-
24	JER	>48	1.98 / 1.73	ND	6	-	0	0	-
25	HOL	>48	1.79 / 2.07	ND	4	+	0	0	-
26	HOL	36	0.14 / 0.05	0.0	6	-	+	0	-
27	JER	>48	0.17 / 0.05	0.0	6	-	+	0	-
28	HOL	>48	2.6 / 2.33	0.04	6	+	+	0	-
29	HOL	>48	2.26 /2.0	0.51	7	+	+	+2	+2
30	HOL	28	0.32 / 0.4	0.06	8	-	+	0	-
31	J/H	>48	0.11 / 0.21	0.06	5	-	0	0	-
32	HOL	>48	1.98 / 1.92	3.08	8	+	+	0	-
33	HOL	>48	0.21 / 0.16	0.0	7	-	+	0	-
34	JER	>48	0.53 / 0.17	1.68	7	+	++	+1	+1
35	JER	>48	1.95	2.35	6	-	+	0	-
36	JER	>48	2.3 / 2.13	0.03	7	-	++	0	-
37	JER	>48	0.16 / 0.18	0.0	6	-	0	0	-
38	HOL	>48	2.3 / 2.12	1.14	8	+	+	0	-
39	JER	>48	0.13 / 0.06	0.0	7	-	+	0	-
40	JER	30	0.05 / 0.13	0.0	7	-	++	0	-
41	HOL	>48	2.07/0.18	ND	mum	ND	ND		ND

Variables	Positive (%)	Negative (%)	Total	*p-value* ¹ 1 Chi-square by Yates corrected.	OR	OR95%
Age-cows (months)
24-36	00(0.0)	10(100)	10(24.4)	0.0001^* * Fisher exact test.	0.00	(0.00, 0.31)
>36	21(67.7)	10(32.3)	31 (75.6)
Total	21(51.2)	20(48.8)	41² 2 One animal (N.15) did not have serum tested because of contaminated serum.

Trimester of gestation
Second(4-6)	8(61.5)	5(38.5)	13(34.2)	0.84	2.40	(0.50, 11.9)
Third (>7)	10 (40.0)	15(60.0)	25(65.8)
Total	18(47.4)	20(52.6)	38³ 3 Three cows did not have their gestational age determined because fetuses were mummified.

Breeds
Jersey	11 (50.0)	11 (50.0)	22(55%)	0.97	0.8	(0.19, 3.34)
Holstein	10 (55.5%)	8(44.5%)	18 (45%)
Total	19 (47.5%)	21 (52.5)	40⁴ 4 Two cows were not used N.15 (contaminated serum) and N.32 (a crossbreed Jersey/Holstein).

Dams	Fetuses				*p-value*	OR	OR95%
	ELISA
	Positives (%)	Negatives (%)		T(%)
+	8(61.5)	5 (38.5)		13(43.3)	0.0002¹ 1 Fischer exact.	UN	Not accurate
-	0(0)	17(100)		17(56.7)
T(%)	8(26.7)	22(73.3)		30


	PCR
+	11(64.7)	6(35.3)	17(47.2)
-	3(15.8)	16(84.2)	19(52.8)		0.007² 2 Chi-square by Yates corrected.	9.78	(1.63, 67.5)
T(%)	14(38.8)	22(61.2)	36
	IHC
+	7(41.2)	10(58.8)	17(47.2)		0.05¹	5.9	(0.85, 52)
-	2(10.5)	17(89.5)	19(52.8)
T(%)	9(25.0)	27(75.0)	36

vertical_align_top file_download show_chart

more_horizclose
- image
- translate
- link
- article
- vertical_align_top
- file_download
- show_chart
- image
- translate
- link
- article

location_on

Colégio Brasileiro de Parasitologia Veterinária FCAV/UNESP - Departamento de Patologia Veterinária, Via de acesso Prof. Paulo Donato Castellane s/n, Zona Rural, , 14884-900 Jaboticabal - SP, Brasil, Fone: (16) 3209-7100 RAMAL 7934 - Jaboticabal - SP - Brazil
E-mail: cbpv_rbpv.fcav@unesp.br

rss_feed Acompanhe os números deste periódico no seu leitor de RSS

Acessibilidade / Reportar erro

[TFN1] ¹
S/P from ELISA (Idexx) S/P ≥ 0.50 (positive) and <0.50 (negative).

[TFN2] ²
Mummified fetuses (mum) did not have their tissues and blood tested, and age determined.

[3] ND = not done; - (negative); + (positive); JER (Jersey); HOL (Holstein); J/H (Jersey/Holstein); Legend for histopathology: 0, absent; +, discrete; ++, moderate; +++, severe; IHC = Immunohistochemistry; +1= positive in the myocardium; +2=positive in the cerebrum.