Genetic diversity and molecular survey of Trypanosoma (Megatrypanum ) theileri in cattle in Brazil’s western Amazon region

Pacheco, Thábata dos Anjos; Marcili, Arlei; Costa, Andrea Pereira da; Witter, Rute; Melo, Andréia Lima Tomé; Boas, Ricardo Vilas; Chitarra, Cristiane Silva; Dutra, Valéria; Nakazato, Luciano; Pacheco, Richard de Campos

doi:10.1590/S1984-296120180049

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Short Communication • Rev. Bras. Parasitol. Vet. 27 (4) • Oct-Dec 2018 • https://doi.org/10.1590/S1984-296120180049 linkcopy

Genetic diversity and molecular survey of Trypanosoma (Megatrypanum ) theileri in cattle in Brazil’s western Amazon region

Diversidade genética e levantamento molecular de Trypanosoma (Megatrypanum) theileri em bovinos na região da Amazônia Ocidental do Brasil

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Trypanosoma (Megatrypanum) theileri is a flagellated protozoan that infects ruminants and it displays high genetic diversity. In this study, we investigated the prevalence rates of this protozoan based on hemoculture and molecular diagnosis. The isolates of T. theileri thus obtained were characterized by molecular markers SSU rDNA and gGAPDH and molecular diagnosis based on Cathepsin L-like gene (PCR-TthCATL). The PCR-TthCATL and hemoculture indicated an overall prevalence rate of 8.13%, and the CATL derived sequence named IB was identified for the first time in cattle in the western Amazon region, as well as IF in Brazil. We also describe a possible new PCR-TthCATL derived sequence in cattle, designated IL.

Keywords: Molecular diagnosis; phylogenetic analysis; hemoculture; Trypanosomatids

Region	No. of farms	No. of sampled farms	No. of positives farms		N. of cows	No. of sampled cows	No. of positives cows
Region	No. of farms	No. of sampled farms	PCR-TthCATL	Hemoculture	N. of cows	No. of sampled cows	PCR-TthCATL	Hemoculture
			Positive (%)	Positive (%)			Positive (%)	Positive (%)
1	122	9	2 (22.22)	2 (4.44)	5246	45	3 (6.67)	2 (4.44)
2	124	9	7 (77.77)	0 (0)	5952	45	10 (22.22)	0 (0)
3	54	4	0 (0)	0 (0)	2268	20	0 (0)	0 (0)
4	176	13	8 (61.54)	6 (45.15)	8096	65	15 (23.08)	11 (16.92)
5	143	11	3 (27.27)	0 (0)	4433	55	3 (5.45)	0 (0)
6	237	18	7 (38.89)	0 (0)	8532	90	8 (8.89)	0(0)
Total	856	64	27 (42.19)	8 (12.5)	34527	320	39 (12.19)	13 (4.06)

Trypanosoma theileri isolates and blood samples	Genotypes and CATL derived sequence based on DNA sequences			GenBank accession numbers
Trypanosoma theileri isolates and blood samples	CATL	SSU rDNA	gGAPDH	CATL	SSU rDNA	gGAPDH
CBT 111	IIB	IIB	IIB	KU587675	KU587637	KU587656
CBT 112	IIB	IIB	IIB	KU587676	KU587638	KU587657
CBT 113	IB	IB	IB	KU587677	KU587639	KU587658
CBT 114	IB	IB	IB	KU587678	KU587640	KU587659
CBT 115	IIB	IIB	IIB	KU587679	KU587641	KU587660
CBT 119	IIB	IIB	IIB	KU587680	KU587642	KU587661
CBT 120	IIB	IIB	IIB	KU587681	KU587643	KU587662
CBT 121	IIB	IIB	IIB	KU587682	KU587644	KU587663
CBT 122	IB	IB	IB	KU587683	KU587645	KU587664
CBT 123	IIB	IIB	IIB	KU587684	KU587646	KU587665
CBT 136	IB	IB	IB	KU587685	KU587647	KU587666
CBT 137	IB	IB	IB	KU587686	KU587648	KU587667
CBT 155	IB	IB	IB	KU587687	KU587649	KU587668
P12B06	IF	-	-	KU587688	-	-
P23B01	IB	-	-	KU587689	-	-
P23B03	IB	-	-	KU587690	-	-
P58B06	IB	-	-	KU587691	-	-
P60B02	IL	-	-	KU587692	-	-

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Colégio Brasileiro de Parasitologia Veterinária FCAV/UNESP - Departamento de Patologia Veterinária, Via de acesso Prof. Paulo Donato Castellane s/n, Zona Rural, , 14884-900 Jaboticabal - SP, Brasil, Fone: (16) 3209-7100 RAMAL 7934 - Jaboticabal - SP - Brazil
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