Variáveis associadas à prevalência de anticorpos anti- <i>Leishmania</i> spp. em cães na tríplice fronteira de Foz do Iguaçu, Paraná, Brasil

Dias, Renata Cristina Ferreira; Thomaz-Soccol, Vanete; Pasquali, Aline Kuhn Sbruzzi; Alban, Silvana Maria; Fendrich, Ricardo Cancio; Pozzolo, Eliane Maria; Chiyo, Luciana; Bisetto Júnior, Alceu; Ferreira, Fernanda Pinto; Desto, Stela Silva; Freire, Roberta Lemos; Mitsuka-Breganó, Regina; Navarro, Italmar Teodorico

doi:10.1590/S1984-296120180055

Renata Cristina Ferreira Dias ^** Corresponding author: Renata Cristina Ferreira Dias. Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, Km 380, Campus Universitário, CEP 86057-970, Londrina, PR, Brasil. e-mail: rcfdias14@gmail.com

Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina – UEL, Londrina, PR, Brasil

Vanete Thomaz-Soccol

Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná – UFPR, Curitiba, PR, Brasil

Aline Kuhn Sbruzzi Pasquali

Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná – UFPR, Curitiba, PR, Brasil

Silvana Maria Alban

Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná – UFPR, Curitiba, PR, Brasil

Ricardo Cancio Fendrich

Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná – UFPR, Curitiba, PR, Brasil

Eliane Maria Pozzolo

9° Regional de Saúde, Foz do Iguaçu, PR, Brasil

Luciana Chiyo

Centro de Controle de Zoonoses, Prefeitura de Foz do Iguaçu, Foz do Iguaçu, PR, Brasil

Alceu Bisetto Júnior

Secretaria da Saúde do Estado do Paraná, Curitiba, Paraná, Brasil

Fernanda Pinto Ferreira

Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina – UEL, Londrina, PR, Brasil

Stela Silva Desto

Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina – UEL, Londrina, PR, Brasil

Roberta Lemos Freire

Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina – UEL, Londrina, PR, Brasil

Regina Mitsuka-Breganó

Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina – UEL, Londrina, PR, Brasil

Italmar Teodorico Navarro

Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina – UEL, Londrina, PR, Brasil

* Corresponding author: Renata Cristina Ferreira Dias. Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, Km 380, Campus Universitário, CEP 86057-970, Londrina, PR, Brasil. e-mail: rcfdias14@gmail.com

Region	Number of serum samples	DPP^® %	ELISA %	RIFI %	ELISA¹ RIFI %	DPP^®2 ELISA %	Prev.¹	Prev.²
Bordering Argentina	285	3.2	30.5	4.6	5	7	1.8%	2.5%
Bordering Paraguay	396	7.6	21.2	4.3	12	20	3.0%	5.1%

Variables	Prot.¹			Prot.²
Variables	Seroreactive/Total (%)	P-value	OR (95%CI)	Seroreactive/Total (%)	P-value	OR (95%CI)
Place of residence
Urban area	5/226 (2.2)	0.6553⁽¹⁾	Ind.**	5/226 (2.2)	0.8406( ¹ (1) Fisher's exact test. )	0.61 (0.10-6.59)
Rural area	0/56 (0.0)			2/56 (11.2)
Presence of woods close to the household
Yes	4/241 (1.7)	>0.9999⁽¹⁾	Ind.**	5/241 (2.1)	>0.9999⁽¹⁾	0.62 (0.07-5.44)
No	0/30 (0.0)			1/30 (3.3)
Distance of woods from household
0-300 meters ^* * = Reference category;	0/117 (0.0)			4/117 (3.4)
300-500 meters	3/50 (6.0)	0.0514⁽¹⁾	0 (0.0-1.01)	0/50 (0.0)	0.4744⁽¹⁾	Ind.**
More than 500 meters	1/61 (1.6)	0.6854⁽¹⁾	0 (0.0-20.3)	1/61 (1.6)	0.8810⁽¹⁾	2.1(0.20-106.3)
Presence of other animals
Yes	4/201 (2.0)	>0.9999⁽¹⁾	1.62 (0.16-80.98)	7/201 (3.5)	0.1813⁽¹⁾	Ind.**
No	1/81 (1.2)			0/81 (0.0)
Presence of organic matter in the backyard
Yes	5/217 (2.3)	0.5533⁽¹⁾	Ind. ^ = Undefined.	6/217 (2.8)	>0.9999⁽¹⁾	1.76 (0.21-82.3)
No	0/63 (0.0)			1/63 (1.6)
Public collection of domestic waste
Yes	5/241 (2.08)	>0.9999⁽¹⁾	Ind.**	5/241 (2.08)	0.4020⁽¹⁾	0.33 (0.05-3.61)
No	0/33 (0.0)			2/33 (6.1)
Dog with identified breed
Yes	1/62 (1.6)	>0.9999⁽¹⁾	0.85 (0.02-8.79)	0/62 (0.0)	0.3220⁽¹⁾	0.0 (0.0-2.35)
No	4/211 (1.9)			7/211 (3.3)
Gender
Male	2/139 (1.4)	>0.9999⁽¹⁾	0.67 (0.06-5.97)	5/139 (3.6)	0.4355⁽¹⁾	2.59 (0.41-27.59)
Female	3/141 (2.1)			2/141 (1.4)
Age group
≤ 1 year*	0/54 (0.0)			0/54 (0.0)
2 ┤8 years	4/199 (2.0)	0.7606⁽¹	0 (0.0-5.62)	6/199 (3.0)	0.4659⁽¹⁾	0 (0.0-2.38)
> 8 years	1/23 (4.4)	0.5974⁽¹⁾	0 (0.0-16.61)	1/23 (4.4)	0.5974⁽¹⁾	0 (0.0-16.61)
Dog came from another city
Yes	0/18 (0.0)	>0.9999⁽¹⁾	0 (0.0-16.35)	0/18 (0.0)	>0.9999⁽¹⁾	0.0 (0-10.43)
No	5/257 (2.0)			7/257 (2.7)

Variables	Prot.¹			Prot.²
Variables	Seroreactive/Total (%)	P-value	OR (95%CI)	Seroreactive/Total (%)	P-value	OR (95%CI)
Place of residence
Urban area	10/364 (2.8)	0.5034⁽²⁾	0.43 (0.08-4.17)	16/364 (4.4)	0.1354( ² (2) Fisher's exact test. )	0.32 (0.10-1.42)
Rural area	2/32 (6.3)			4/32 (12.5)
Presence of woods close to the household
Yes	11/345 (3.2)	>0.9999⁽²⁾	1.61 (0.23-70.86)	17/345 (4.9)	0.9526⁽²⁾	0.81 (0.22-4.49)
No	1/50 (5.0)
Distance of woods from household
0-300 meters ^* * = Reference category;	7/158 (4.4)			11/158 (7.0)
300-500 meters	2/97 (2.1)	0.5323⁽²⁾	2.2 (0.41-22.1)	6/97 (6.2)	>0.9999⁽²⁾	1.13(0.34-3.87)
More than 500 meters	1/80 (1.3)	0.3718⁽²⁾	3.7 (0.46-30.29)	0/80 (0.0)	0.0195⁽²⁾	Ind. ^ = Undefined
Presence of other animals
Yes	6/253 (2.4)	0.4469⁽²⁾	0.54 (0.14-2.08)	10/253 (4.0)	0.2566⁽²⁾	0.54 (0.19-1.48)
No	6/140 (4.3)			10/140 (7.1)
Presence of organic matter in the backyard
Yes	10/328 (3.1)	>0.9999⁽²⁾	1.02 (0.21-9.81)	17/328 (5.2)	>0.9999⁽²⁾	1.17 (0.32-6.4)
No	2/67 (3.0)			3/67 (4.5)
Public collection of domestic waste
Yes	7/371 (1.9)	0.0004⁽¹⁾	0.07 (0.02-0.30)	14/371 (3.8)	0.0007( ¹ (1) Pearson's chi-squared test; )	0.11 (0.03-0.38)
No	5/22 (22.7)			6/22 (27.3)
Dog with identified breed
Yes	4/144 (2.8)	>0.9999⁽²⁾	0.86 (0.19-3.28)	5/144 (3.5)	0.3838⁽¹⁾	0.56 (0.18-1.53)
No	8/249 (3.2)			15/249 (6.0)
Gender
Male	6/181 (3.3)	0.9929⁽¹⁾	1.19 (0.36-3.98)	10/181 (5.5)	0.8687⁽¹⁾	1.20 (0.48-3.29)
Female	6/215 (2.8)			10/215 (4.7)
Age group
≤ 1 year ^* * = Reference category;	0/69 (0.0)			1/69 (1.5)
2 ┤8 years	9/277 (3.3)	0.2631⁽²⁾	0.0 (0.0-2.02)	16/277 (5.8)	0.2263⁽²⁾	0.24 (0.01-1.6)
> 8 years	3/49 (6.1)	0.1381⁽²⁾	0.0 (0.0-1.69)	3/49 (6.1)	0.2263⁽²⁾	0.23 (0.004-2.9)
Dog came from another city
Yes	0.0/16 (0.0)	>0.9999⁽²⁾	0.0 (0.0-9.79)	0/16 (0.0)	0.8330⁽²⁾	0.0 (0.0-4.90)
No	11/367 (3.0)			20/367 (5.5)

Variables	Number (%) of residences on the International border
Variables	Argentina	Paraguay
Place of residence
Urban area	226 (80.1)	364 (91.9)
Rural area	56 (19.9)	32 (8.1)
Presence of woods close to the household
Yes	241 (88.9)	345 (87.3)
No	30 (11.1)	50 (12.7)
Distance of woods from household
0 – 300 meters	117 (51.3)	158 (47.2)
300 – 500 meters	50 (21.9)	97 (28.9)
More than 500 meters	61 (26.8)	80 (23.9)
Presence of other animals
Yes	201 (71.3)	253 (64.4)
No	81 (28.7)	140 (35.6)
Type of animals
Wild animal	32 (11.2)	4 (1.0)
Mouse	8 (2.8)	8 (2.0)
Equine	68 (23.9)	66 (16.7)
Hen	55 (19.3)	21 (5.3)
Opossum	63 (22.1)	198 (50.0)
Cat	9 (3.2)	0 (0.0)
Monkey	0 (0.0)	2 (0.5)
Mule	107 (37.5)	80 (20.2)
Presence of organic matter in the backyard
Yes	217 (77.5)	328 (83.0)
No	63 (22.5)	67 (17.0)
Type of organic matter
Leaf accumulation	112 (39.3)	169 (42.7)
Vegetable garden	40 (14.0)	59 (14.9)
Garden	115 (40.4)	181 (45.7)
Waste	43 (15.1)	67 (16.9)
Orchard	81 (28.4)	64 (16.2)
Dog with identified breed
Yes	62 (22.7)	144 (36.6)
No	211 (77.3)	249 (63.4)
Gender
Male	139 (49.6)	181 (45.7)
Female	141 (50.4)	215 (54.3)
Age group
≤ 1 year	54 (19.6)	69 (17.5)
2 ┤8 years	199 (72.1)	277 (70.1)
> 8 years	23 (8.3)	49 (12.4)
Dog came from another city
Yes	18 (6.5)	16 (4.2)
No	257 (93.5)	367 (95.8)

[1] * Corresponding author: Renata Cristina Ferreira Dias. Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, Km 380, Campus Universitário, CEP 86057-970, Londrina, PR, Brasil. e-mail: rcfdias14@gmail.com

Brasil

Brasil

Variáveis associadas à prevalência de anticorpos anti- Leishmania spp. em cães na tríplice fronteira de Foz do Iguaçu, Paraná, Brasil

Resumo