This work aimed to study chances in the isoenzymatic expression of malate dehydrogenase and acid phosphatase of pea seeds submitted to different pre-hydration methods prior to electrical conductivity test. Pea seed lots from the cvs Axé and Maria were characterized for their water content, germination, first germination counting, accelerated aging, electrical conductivity and field seedling emergence. The seeds of each lot were pre-hydrated using two methods: saturated atmosphere and humidified substrate until 10, 12 and 14% of water content. The results showed differential expression of acid phosphatase, which depended on the cultivar, on the pre-hydration method and on the final water content. These results indicated differences in seed cellular membrane restructuring system between cultivars with smooth and wrinkled tegument and suggested the needs of improvement in the pre-hydration methods of pea seeds when submitted to the electrical conductivity test.
Pisum sativum; membrane integrity; malate dehydrogenase; acid phosphatase
