To evaluate physiologic and biochemical alterations in bean seeds during storage in environmental conditions not controlled of temperature and relative humidity of the air, the present work was accomplished. It were used the cultivars: Iapar 44, Macotaço, TPS Bonito, TPS Bionobre and TPS Nobre. The seeds were stored in not controlled environmental conditions in Santa Maria -RS, for eight months. The physiologic evaluations consisted of the germination test, first germination count, electric conductivity, seedling and root length, electric conductivity and emergency in field. The biochemical evaluations of the seeds were obtained by the technique of electrophoresis of enzymatic systems, being evaluated the activity of the enzymes acid fosfatase, malate dehydrogenase, glutamate dehydrogenase and esterase. All the evaluations were accomplished before and each two months during the storage. The results allow to conclude the activity of the enzymes malate and glutamate dehydrogenase are stable during the storage in not controlled environmental conditions in cultivars vigorous (TPS Bionobre, TPS Nobre and Iapar 44); the esterase enzyme has its activity increased during storage in not controlled environmental conditions, independently of bean seeds physiologic quality; potentials of storage under not controlled environmental conditions are different in the cultivars TPS Bionobre, TPS Nobre, TPS Bionobre, Macotaço and Iapar 44.
Phaseolus vulgaris; enzyme; deterioration
