A novel method for reliable and rapid detection of BC, BLAD, CVM, and DUMPS in cattle

ABSTRACT

This study aimed to optimize a reliable and rapid genotyping assay to detect the carriers of bovine citrullinemia (BC), bovine leukocyte adhesion deficiency (BLAD), complex vertebral malformation (CVM), and deficiency of uridine monophosphate synthase (DUMPS) in cattle populations. We developed real-time polymerase chain reaction (RT-PCR)-based assays to distinguish wild-type and defective alleles of BLAD, CVM, BC, and DUMPS. Twenty-four bulls in the International Center for Livestock Research and Training were genotyped. At the same time, genotyping was performed for DUMPS and BC using PCR-RFLP, and sequencing was performed for all diseases and compared with the RT-PCR kit we developed. None of the bulls carried mutant alleles of these hereditary autosomal recessive lethal defects. It takes only 2 h for the assay to be completed, including DNA extraction from the sample. These consequences indicate that RT-PCR is an easy, reliable, and rapid method for detecting BLAD, CVM, BC, and DUMPS carriers. Studies show a high frequency of mutant alleles of these genetic defects that cause genetic diseases, and this requires routine test systems to eradicate genetic diseases of economic importance.

bovine citrullinemia; bovine leukocyte adhesion deficiency; complex vertebral malformation; deficiency of uridine monophosphate synthase; genetic disease in cattle; real-time PCR-based assay