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Genetic variability in natural populations of Bromus auleticus Trin. ex Nees (Poaceae) using isozymes and RAPD markers

Bromus auleticus is a perennial, native species of the state of Rio Grande do Sul, which has demonstrated adaptability, potential yield of good quality forage as well as high morphological diversity. This work has analyzed band patterns from enzymatic systems and RAPD markers, with the objective to access the genetic variability in 16 accessions of Bromus auleticus from Rio Grande do Sul. The variability was evaluated using Jaccard similarity index. The method of grouping based on the average (UPGMA) was used in cluster analyses. The similarity index ranged from 0.0 to 0.50 using isozumes and from 0.15 to 0.71 using RAPD markers. The data have been efficient for the formation of different groups, indicating the genetic variability of the accession analyzed. However, these groupings have little relationship with the respective collecting places. The genetic variability of the accessions is important to the genetic improvement, allowing future genotype selection based on their respective performances. The simultaneous utilization of isozymes and RAPD was efficient in characterizing the genetic diversity of the accessions evaluated.

Bromus auleticus; isozymes; RAPD; genetic variability; native forages


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