This study was run to evaluate the physiological response and expression of hepatic and brain Hsp70, of 20 Naked neck (Na/na) and Hubbard-Petersen broiler chicks (2 to 5 days of age), exposed or not to 36-37°C. The trial was conducted during four days and the birds were exposed to high temperature during 5 hours. Liver and brain tissue samples were collected at the end of the stress period and analyzed for Western Blotting for determination of Hsp70 level. No association between body weight loss and the other variables were observed. Increasing the variation in cloacal temperature resulted in an increase in Hsp70 hepatic and brain tissue levels. Results indicate that brain Hsp70 levels were correlated with variation in cloacal temperature, concerning the chicks subjected to heat stress, but it can not be stated that it is an indicative of thermotolerance by the birds.
broiler chicks; cloacal temperature; heat stress; liver and brain Hsp70 levels
