Genetic variation in broods of Anopheles cruzii was studied. Mosquitoes were collected in Morretes and Paranaguá (two municipalities in the State of Paraná, Southern Brazil) from which broods were reared. DNA was collected from these broods, from which six random-sequence primers were used in PCR. In within-brood analyses, all markers revealed a high-degree of polymorphisms within the broods. Detailed studies of the within-brood variability will help solve taxonomic problems in this group, since differences in fragment patterns are used to distinguish species.
Anopheles bellator; malaria vector; random amplified polymorphic DNA
