A new species of Pygmy-Owl, genus Glaucidium, is described from the Amazonian forest. It is separated from G. minutissimum, now restricted to South-eastern Brazil and Central America, by its voice and shorter tail. The type-locality is from Rondônia, but the species seems widespread, with geographical races awaiting description from northern Pará, Northeastern Brazil, and possibly Central America. A detailed survey of this overlooked canopy-living Pygmy-Owl is under way.