Image analysis in the evaluation of the physiological potential of maize seeds<sup>1</sup>

Pinto, Crislaine Aparecida Gomes; Carvalho, Maria Laene Moreira de; Andrade, Dayliane Bernardes de; Leite, Eva Rezende; Chalfoun, Igor

doi:10.5935/1806-6690.20150011

Acessibilidade / Reportar erro

Brasil

Revista Ciência Agronômica

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Crop Science • Rev. Ciênc. Agron. 46 (2) • Apr-Jun 2015 • https://doi.org/10.5935/1806-6690.20150011 copy

Image analysis in the evaluation of the physiological potential of maize seeds¹

Análise de imagens na avaliação do potencial fisiológico de sementes de milho

Authorship SCIMAGO INSTITUTIONS RANKINGS

The Seed Analysis System (SAS) is used in the image analysis of seeds and seedlings, and has the potential for use in the control of seed quality. The aim of this research was to adapt the methodology of image analysis of maize seedlings by SAS, and to verify the potential use of this equipment in the evaluation of the physiological potential of maize seeds. Nine batches of two maize hybrids were characterised by means of the following tests and determinations: germination, first count, accelerated ageing, cold test, seedling emergence at 25 and 30ºC, and speed of emergence index. The image analysis experiment was carried out in a factorial scheme of 9 batches x 4 methods of analysis of the seedling images (with and without the use of NWF as substrate, and with and without manual correction of the images). Images of the seedlings were evaluated using the average lengths of the coleoptile, roots and seedlings; and by the automatic and manual indices of vigour, uniformity and growth produced by the SAS. Use of blue NWF afffects the initial development of maize seedlings. The physiological potential of maize seeds can be evaluated in seedlings which are seeded on white paper towels at a temperature of 25 °C and evaluated on the third day. Image analysis should be carried out with the SAS software using automatic calibration and with no correction of the seedling images. Use of SAS equipment for the analysis of seedling images is a potential tool in evaluating the physiological quality of maize seeds.

Zea mays ; Vigour; SAS

Parameter	Weight
Growth (wC)	Variable
Uniformity (wU)	Variable
Length of hypocotyl (wl_h)	2.5
Length of root (wl_r)	5.0
Standard deviation of hypocotyl length (ws_r¬)	0.75
Standard deviation of root length (ws_r)	0.5
Standard deviation of total length (ws_total)	2.5
Standard deviation of the hypocotyl length to root length ratio (w s_r/h)	50
Number of dead seeds (wD)	0

Batch	1st C (%)	G (%)	AA (%)	CT (%)	E25 (%)	E30 (%)	SEI 25	SEI 30
1	76 c	92 b	85 b	89 b	97 b	98 a	15.35 b	15.92 b
2	77 c	93 b	91 b	91 b	95 c	95 a	15.12 b	15.68 b
3	78 c	94 b	93 a	89 b	96 b	95 a	15.22 b	15.88 b
4	89 a	97 a	96 a	94 a	99 a	98 a	16.01 a	16.41 a
5	86 a	96 a	95 a	95 a	99 a	99 a	16.40 a	16.66 a
6	90 a	97 a	97 a	94 a	99 a	99 a	16.05 a	16.71 a
7	82 b	93 b	89 b	88 b	95 c	95 a	14.68 c	15.44 c
8	77 c	93 b	86 b	86 b	96 b	95 a	14.67 c	15.36 c
9	82 b	95 b	88 b	87 b	97 b	96 a	14.98 b	15.52 c
CV(%)	3.99	1.86	5.73	3.54	1.25	4.00	2.05	1.66

Batch	1st C (%)	G (%)	AA (%)	CT (%)	E25 (%)	E30 (%)	SEI 25	SEI 30
A	61 c	93 a	86 a	94 a	96 a	97 a	13.91 a	13.92 a
B	62 c	88 b	91 a	87 a	96 a	96 a	13.91 a	13.79 a
C	65 c	89 b	89 a	92 a	96 a	95 a	13.93 a	13.78 a
D	69 b	94 a	83 b	79 b	96 a	96 a	13.77 a	13.79 a
E	61 c	94 a	77 b	80 b	97 a	95 a	13.83 a	13.67 a
F	70 b	95 a	82 b	80 b	98 a	97 a	13.94 a	13.76 a
G	71 b	94 a	78 b	91 a	96 a	95 a	13.93 a	13.75 a
H	75 b	94 a	75 b	88 a	96 a	95 a	13.85 a	13.69 a
I	82 a	92 a	83 b	93 a	95 a	97 a	13.81 a	13.86 a
CV(%)	6.92	2.35	5.73	4.62	1.47	1.48	1.78	1.39

Parameter	Weight
Parameter	Hybrid 1	Hybrid 2
Growth (wC)	83.85629072	108.4964549
Uniformity (wU)	83.85629072	108.4964549
Length of hypocotyl (wl_h)	-2.823343321	9.322130566
Length of root(wl_r)	1.762574061	-2.252439132
Standard deviation of hypocotyl length (ws_h-)	15.69882055	3.365555998
Standard deviation of root length (ws_r)	8.572735849	-6.881685425
Standard deviation of total length (ws_tota_l)	-11.61613778	-0.279812909
Standard deviation of the hypocotyl length to root length ratio (w s_r/h)	0.016115942	-2.058411161
Number of dead seeds (wD)	0.788050143	3.716112821

Batch	Methods of image analysis using SAS
Batch	No NWF with correction	No NWF no correction	NWF with correction	NWF no correction
1	4.44 cA	4.44 cA	4.44 cA	4.44 cA
2	2.25 cA	5.21 cA	5.05 bA	4.96 bA
3	4.78 cA	4.73 cA	5.08 bA	5.10 bA
4	7.66 aA	7.49 aB	6.47 aB	6.51 aB
5	7.78 aA	7.76 aB	6.38 aB	6.32 aB
6	7.13 aA	7.13 aA	6.93 aA	6.95 aA
7	5.93 bA	6.01 bA	5.20 bB	5.27 bB
8	6.15 bA	6.26 bA	4.92 bB	4.96 bB
9	5.80 bA	5.85 bA	5.34 bA	5.41 bA
CV(%)		9.22

Batches	-----------Length (cm)----------		-------------------------------------Index-------------------------------------
Batches	Coleoptile	Total	VA	VM	U	C
1	0.94 b	5.41 e	88.2 b	40.7 d	78.1 a	24.7 d
2	1.16 a	6.29 c	88.7 b	43.7 b	79.0 a	28.5 c
3	1.02 b	5.95 d	88.7 b	42.5 c	78.3 a	27.2 c
4	1.16 a	8.19 a	93.1 a	48.7 a	73.5 b	38.1 a
5	1.29 a	8.35 a	93.8 a	50.1 a	77.2 a	38.5 a
6	0.23 a	8.26 a	93.1 a	49.1 a	74.5 b	38.2 a
7	1.25 a	6.85 b	88.6 b	45.0 b	77.5 a	31.1 b
8	1.22 a	6.79 b	88.7 b	44.6 b	76.5 a	30.9 b
9	1.21 a	6.81 b	89.2 b	44.4 b	75.6 b	31.0 b
CV(%)	13.81	9.62	1.57	5.42	5.42	9.41

Method of Image	--------Length (cm)---------		-----------------------------Index----------------------------
Analysis	Coleoptile	Total	VA	VM	U	C
no NWF with correction	1.33 a	7.43 a	89.7 b	47.5 a	79.3 a	30.1 a
no NWF no correction	1.44 a	7.54 a	91.2 a	46.7 a	76.3 b	30.1 a
NWF with correction	0.96 b	6.50 b	89.2 b	44.4 b	77.8 b	33.8 b
NWF no correction	0.93 b	6.48 b	90.8 a	43.1 c	73.4 c	34.1 b
CV(%)	13.81	9.62	1.57	5.42	5.42	9.41

Batch	-----------------Length (cm)-------------------			-------------------------------Index-----------------------------
Batch	Coleoptile	Root	Total	VA	VM	U	C
A	1.17 b	5.46 c	6.63 c	91.2 a	44.4 b	77.6 a	30.2 c
B	1.18 b	5.18 c	6.36 c	92.4 a	43.5 b	77.7 a	28.8 c
C	1.18 b	5.36 c	6.55 c	91.2 a	44.2 b	77.8 a	29.8 c
D	0.97 c	6.08 b	7.04 b	85.0 c	44.9 b	73.3 b	32.8 b
E	0.78 d	5.36 c	6.14 c	83.9 c	41.2 c	70.3 b	28.7 c
F	1.06 c	6.22 b	7.28 b	84.0 c	45.2 b	71.8 b	33.8 c
G	1.13 b	5.98 b	7.11 b	87.8 b	45.7 b	76.0 a	32.7 b
H	1.35 a	6.45 a	7.80 a	88.9 b	47.8 a	76.3 b	35.6 a
I	1.39 a	6.54 a	7.93 a	89.7 b	48.5 a	77.2 b	36.2 a
CV(%)	15.71	8.73	9.39	3.20	5.52	6.04	9.06

Method of Image	Length (cm)			Index
Analysis	Coleoptile	Root	Total	VA	VM	U	C
no NWF with correction	0.93 b	5.18 b	6.11 b	89.0 a	43.0 b	77.5 b	28.2 b
no NWF no correction	0.99 b	5.21 b	6.19 b	89.2 a	42.6 b	75.4 a	28.5 b
NWF with correction	1.33 a	6.50 a	7.80 a	88.0 b	47.7 a	75.5 a	35.8 a
NWF no correction	1.29 a	6.51 a	7.82 a	86.7 b	46.9 a	72.8 b	35.8 a
CV(%)	15.71	8.73	9.39	3.20	5.52	6.04	9.06

Universidade Federal do Ceará Av. Mister Hull, 2977 - Bloco 487, Campus do Pici, 60356-000 - Fortaleza - CE - Brasil, Tel.: (55 85) 3366-9702 / 3366-9732, Fax: (55 85) 3366-9417 - Fortaleza - CE - Brazil
E-mail: ccarev@ufc.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] * Autor para correspondência

Brasil

Brasil

Image analysis in the evaluation of the physiological potential of maize seeds1

Análise de imagens na avaliação do potencial fisiológico de sementes de milho

Image analysis in the evaluation of the physiological potential of maize seeds¹