Provenance, viability and physical quality in seeds of Panicum maximum ‘Mombasa’ABSTRACT
Identifying areas that would enable the production of highly pure and viable seeds is important for the logistics of companies that act in the seed sector. The aim of the present study was to evaluate the effect of provenance on the viability and physical quality of seeds of Panicum maximum ‘Mombasa’. Nineteen seed batches from the states of São Paulo (six from Auriflama and three from Guzolândia) and Goiás (eight from Quirinópolis and two from Serranópolis) were evaluated using the following parameters: water content, viability by tetrazolium test, physical purity, PLS, 1000-seed weight and number of other seeds. Physical quality and viability in seeds of P. maximum ‘Mombasa’ are affected by their provenance. All the seed batches showed a sufficient level of viability to meet marketing standards. Based on physical purity, seed samples from Quirinópolis, Goiás should not be marketed. Based on an examination of noxious seeds, 85% of the seed samples are suitable for marketing.
Keywords: Export; Mombasa Grass; Noxious seeds; PLS
RESUMO
A identificação de áreas que possibilitam a produção de sementes com elevada pureza e viabilidade é importante para a logística das empresas do setor sementeiro. Nesse sentido, o objetivo desta pesquisa foi avaliar o efeito da procedência na viabilidade e na qualidade física de sementes de Panicum maximum cv. Mombaça. Dezenove lotes de sementes procedentes dos Estados de São Paulo (seis de Auriflama e três de Guzolândia) e Goiás (oito de Quirinópolis e dois de Serranópolis) foram avaliados por meio dos seguintes parâmetros: teor de água, viabilidade pelo teste de tetrazólio, pureza física, valor cultural, peso de mil sementes e outras sementes por número. A qualidade física e a viabilidade das sementes de P. maximum cv. Mombaça são influenciadas pela procedência. Todos os lotes apresentam sementes com viabilidade suficiente para atender aos padrões de comercialização. Com base na pureza física, o lote dois de Quirinópolis – GO não pode ser comercializado. Com base no exame de sementes nocivas 85% dos lotes podem ser comercializados.
Palavras-chave: Valor cultural; Exportação; Capim-mombaça; Semente nociva
INTRODUCTION
In Latin America and Africa, Panicum maximum ‘Mombasa’ is used for pasture due to its high dry matter production, high forage quality and tolerance to drought, in addition to its ease of management and acceptance by animals (CANTO et al., 2012; DUTRA; RODRIGUES; PEREIRA, 2015).
Human action and the field environment affect the production and quality of grass seeds (SILVA et al., 2014), whereas water availability and temperatures during seed development in the field influence the viability of the seeds (CARVALHO; NAKAGAWA, 2012). In seeds of P. maximum, viability is evaluated by the tetrazolium test, whose results are used together with those of physical purity for marketing purposes (BRASIL, 2008).
Environmental factors, such as excessive rainfall and strong winds, can cause the florets and immature seeds to fall, increasing the impurity of seed batches harvested by sweeping the ground (SOUZA et al., 2015, 2016).
The low physical quality of forage-grass seeds can be attributed to harvesting by ground-sweeping after seedfall (MELO et al., 2018), this results in a mixture of seeds, plant debris, earth and stones (SILVA et al., 2019).
By evaluating physical purity and viability, it is possible to calculate the PLS. This parameter expresses the percentage of viability and purity as a single index, giving the percentage of pure seeds that can germinate under suitable soil conditions (BRASIL, 1992; MARTINS; LAGO; GROTH, 1998).
The PLS is a technical index used by farmers and the producers of forage-grass seeds as a parameter of the quality and market value of seed batches, which aids in decision-making (LAURA et al., 2009). However, Decree no. 16 of 2013 determined that the PLS should no longer appear on seed packaging in Brazil (BRASIL, 2013).
During the physical analysis of batches in the laboratory, seeds of other species may be found, and these reflect the problems that occur during production in the field, such as the inefficient control of invasive plants (ABOUZIENA; HAGGAG, 2016). These plants have the ability to adapt to different climate conditions and develop in different regions (BARROS et al., 2017). However, it is difficult to attribute the occurrence of these plants to climate factors, since most are cosmopolitan (ONEN et al., 2018).
The contamination of plots by noxious plant species should be avoided by choosing areas free of invasive plants and by the use of weed control. The inspection of seed fields also has the aim of avoiding or minimising the occurrence of weeds (BRASIL, 2011). The presence of seeds of other cultivated species, wild species, and restricted noxious or prohibited noxious species can lead to the entire field being rejected when numbers are greater than those established by the Standards for Seed Marketing (BRASIL, 2013).
Various researchers have found differences in the physical quality of P. maximum seeds. However, they did not determine the number of seeds of other species, nor did they relate this quality to the provenance of the seeds (LAURA et al., 2009; MELO et al., 2016a, b).
The aim of the present study, therefore, was to evaluate the effect of provenance on the viability and physical quality of seeds of P. maximum ‘Mombasa’.
MATERIAL AND METHODS
Nineteen batches of P. maximum ‘Mombasa’ seeds were evaluated, from four different locations: Auriflama, São Paulo (SP) (Batches 1 to 6), Guzolândia, SP (Batches 7 to 9), Quirinópolis, Goiás (GO) (Batches 10 to 17) and Serranópolis, GO (Batches 18 and 19). The number of batches by location, the geographic coordinates, altitude and characteristics of the regions where the seed batches were collected are shown in Table 1.
Origin, number of lots (L), geographic coordinates, altitude (Altit.), average temperature (Temp.), accumulated annual precipitation (Precip.), and climate of the P. maximum cv. Mombasa seeds harvest site
After mechanical collection using a groundsweeping harvester, the seeds were submitted to pre-cleaning in the field using a cylindrical sieve machine (typhoon) connected to the power take-off of a tractor. Each 5 kg batch of unprocessed seeds was homogenised, packed in single-sheet paper packaging, and sent to the Seed Analysis Laboratory of the Plant Production Department, Faculty of Agrarian e Veterinary Sciences, Paulista State University, Jaboticabal Campus, SP, where the following parameters were determined:
Moisture content - determined by the oven method at 105 ± 3 ºC for 24 hours. Three subsamples of 2.0 g were weighed on an analytical balance with a readability of 0.001 g, and the data expressed as a percentage on a wet basis (BRASIL, 2009).
Viability by tetrazolium test - four subsamples of 50 seeds were left to soak between moist paper sheets for 18 hours at 30 ºC. The seeds were then split longitudinally through the embryo, and one half of the seed was immersed in a 0.1% tetrazolium solution and kept in a dark room at 37 °C for three hours. After this period, the seeds were washed in running distilled water and the readings taken immediately, classifying the seeds as viable or non-viable. The results were expressed in percentage viability (BRASIL, 2009; TOMAZ et al., 2010).
Physical purity – using two subsamples, each of 2.0 g, weighed on a precision balance (0.001 g). The components of each subsample were separated by pneumatic blower and collected manually. The results were expressed as a percentage of pure seeds, inert matter and other seeds (BRASIL, 2009).
Pure Live Seeds - calculated using the formula: PLS = (TZ × P) / 100, where TZ = viable seeds by tetrazolium test; P = physical purity. The results were expressed as a percentage (BRASIL, 1992).
1000-seed weight - determined using eight subsamples of 100 seeds, individually weighed on a precision analytical balance (0.001 g), with the results expressed in grams (BRASIL, 2009).
Number of other seeds - a 20 g sample of seeds was weighed on a precision analytical balance (0.001 g) and the seeds of other species were identified and counted. The seeds were identified by a specialist in the area, using a stereoscopic microscope, consulting the seed collection and the specialised literature. The results were presented as the number of seeds of each species per sample weight (BRASIL, 2009).
Statistical proceedure: the data were submitted to ANOVA in a completely randomised design. The mean values of the batches were compared using the Scott-Knott test at 5% probability.
RESULTS AND DISCUSSION
The moisture content of the 19 batches of P. maximum ‘Mombasa’ seeds ranged from 9.7% to 11.8% (Table 2). The maximum difference between these values was 2.1%. This difference was considered acceptable by Silva et al. (2019), after finding a difference of 2% in the moisture content of 15 samples of Urochloa decumbens ‘Basilisk’ seeds. It is essential for the moisture content of batches to show similar values in order to obtain valid results from the tetrazolium test and when determining the 1000-seed weight, as the results of these variables may vary depending on the moisture content of the seeds (BATISTA; NUNES; NÓBREGA, 2016; BRASIL, 2009).
Moisture content (MC), viability by tetrazolium test (TZ), pure seeds (P), inert matter (IM), other seeds (OS), PLS and 1000-seed weight (TSW) in 19 seed batches (B) of P. maximum ‘Mombasa’ of varying provenance, 2014/2015 harvest
Seed viability as assessed by the tetrazolium test varied between 70% and 89%. Batches 1 to 4 from Auriflama, Batch 1 from Guzolândia, Batches 1 to 6 from Quirinópolis, and Batch 1 from Serranópolis showed high viability, between 82% and 89%, and were higher than the other batches (Table 2). The viability of the remaining batches was statistically lower, and ranged from 70% to 78%.
Cruz et al. (2020) assessed the influence of climate conditions on the physiological quality of P. maximum ‘Mombasa’ seeds, and concluded that rainfall and high temperatures during seedfall and harvesting are not favourable for producing adequately viable seeds. In the present study, all the seed batches showed adequate viability, greater than 40%, the official standard for marketing seeds of P. maximum (BRASIL, 2008); this was also seen in other studies with seed batches of the same species (FERREIRA; VALLE, 2017; RAGONHA; OLIVEIRA; SILVA, 2018).
Regarding physical purity, the percentage of pure seeds in the batches varied between 49.9% and 94.3% (Table 2). According to Normative Instruction no. 30 of the Ministry of Agriculture, Fisheries and Supply (MAPA), the minimum required value for purity is 50% when marketing a batch of P. maximum seeds (BRASIL, 2008). As such, only Batch 2 from Quirinópolis, was below the required standard (49.9%).
It should be noted that the seed batches used in the present study were unprocessed. The quality, therefore, might be further improved by processing the seeds at the company (MELO et al., 2016a; CRUZ et al., 2020). Batch 5 from Quirinópolis had a purity of 94.3% and would require no processing, an advantage for the seed company.
Based on the percentage of pure seeds, the batches were classified into five groups. The group with the highest purity comprised Batches 1 and 5 from Quirinópolis, which, although statistically different from each other, were the best of all the batches, with values of 86% and 94.3%, respectively. The group with high to medium purity included Batches 1 and 4, harvested in Auriflama, Batch 1 from Guzolândia, Batch 4 and Batch 6 from Quirinópolis, and Batches 1 and 2 from Serranópolis, and had values between 77% and 81%. The group with medium purity comprised Batches 2, 3 and 5 from Auriflama and Batch 8 from Quirinópolis, with values between 68% and 74%. The group with medium to low purity consisted of Batch 6 from Auriflama, and Batches 3 and 7 from Quirinópolis, with values between 56% and 60%. The group with the lowest purity, comprising Batches 2 and 3 from Guzolândia and Batch 2 from Quirinópolis, had values ranging between 50% and 51%.
Differences in physical purity between seed batches may be the result of differences in the production process between operators, even when the producers use the same system of harvesting. Also, the choice of operator is important, since the care taken during production and pre-cleaning in the field had an obvious effect on the purity of the seed batch. The physical quality of unprocessed, freshly harvested seeds of forage grasses is related to management in the field, ground-sweeping procedures when harvesting, and pre-cleaning in the field (SILVA et al., 2019). With mechanical harvesting, periodic adjustment of the machine is essential to prevent impurities from being collected in excess or not being effectively removed, so as not to affect the physical quality of the seeds (MASCHIETTO, 2013).
Physical quality is more dependent on procedures in the field, such as management, and pre-cleaning and adjusting the machines during harvesting, than on the climate conditions at the place of origin of the seeds (SILVA et al., 2019).
Among the impurities found in the 19 batches were straw, stones, clods and immature seeds, including a smaller percentage of the seeds of other species. As such, inert matter was the most common impurity found in the seed batches, with percentagevalues that were almost equal to those found for pure seeds. These results corroborate those from a purity analysis of the seeds of Urochloa decumbens ‘Basilisk’ (SILVA et al., 2019).
As a result, the statistical classification of the seed batches as to inert matter was identical to that obtained for pure seeds. The highest percentage of inert matter was found in Batches 2 and 3 from Guzolândia and Batch 2 from Quirinópolis, with 49.1%, 48.9% and 50.2% inert matter, respectively (Table 2). In those cases, processing at the seed company should be more rigorous, with a greater amount of discarded material than in other batches, since almost half of the material collected (unprocessed seeds) was composed of impurities. Processing removes the impurities from batches to meet the established standards of physical quality for seeds on the domestic market (MELO et al., 2016b).
The worst batches, with the highest incidence of other seeds, were Batch 3 (0.30%) and Batch 6 (0.22%) from Quirinópolis. Other batches such as Batch 1 from Auriflama, and Batches 1 and 4 from Quirinópolis, had a medium number of other seeds, with 0.14%, 0.16% and 0.18%, respectively, as shown in Table 2. The remaining batches had a significantly lower percentage, with values of less than 0.1%. This suggests that invasive plants were better controlled when producing these batches, as verified by Silva et al. (2019).
The presence of diaspores of other species in the seed batches reflects a failure by the operator and technician responsible to control the weeds or inspect the production field (CARVALHO; NAKAGAWA, 2012). This emphasises the importance of carefully choosing operators and technicians who are qualified in seed production.
The PLS expresses the physical and physiological quality of seed batches as a single index. For the 19 seed batches of P. maximum ‘Mombasa’ the PLS varied from 35% to 82%. However, according to Laura et al. (2009), the average value in Brazil is 30%, meaning that for each of the batches under evaluation, the PLS was above the national average.
The batches were classified into four groups based on their PLS. The group with the highest PLS comprised Batches 1 and 5 from Quirinópolis, with values of 76% and 82%, respectively. The group with a medium to high PLS included Batches 1, 3 and 4 from Auriflama, Batch 1 from Guzolândia, Batch 4 and Batch 6 from Quirinópolis, and Batches 1 and 2 from Serranópolis, with values between 61% and 69%. The group with a medium to low PLS comprised Batches 2 and 5 from Auriflama, and Batches 3 and 8 from Quirinópolis, with values between 50% and 56%. The group with the lowest PLS consisted of Batch 6 from Auriflama, Batch 2 and Batch 3 from Guzolândia, and Batches 2 and 7 from Quirinópolis, with values between 35% and 44%.
The PLS expresses viability and purity as a single variable (BRASIL, 1992). The observed values show that each place of origin was suitable for the production of forage-grass seeds, allowing them to be marketed in accordance with the legislation, with no need for processing.
Based on the 1000-seed weight, the batches were classified into five groups: the group with the highest weight comprised Batch 1 from Serranópolis, and Batches 1, 2, 5 and 6 from Quirinópolis, although the first batch was statistically superior to the others. These batches had a 1000-seed weight of between 1.47 and 1.52 g.
The group with a high to medium 1000-seed weight consisted of Batches 2, 3 and 6 from Auriflama and Batch 4 from Quirinópolis, with values between 1.44 and 1.45 g. The group of medium weight included Batch 5 from Auriflama, Batches 2 and 3 from Guzolândia and Batch 2 from Serranópolis, and presented values between 1.40 and 1.41 g. The group with a medium to low weight comprised Batch 4 from Auriflama, Batch 1 from Guzolândia, and Batches 3, 7 and 8 from Quirinópolis, with values between 1.37 and 1.38 g. The group with the lowest weight (1.13 g) consisted of Batch 1 from Auriflama.
The higher seed weights may be due to favourable climate conditions during flowering and seed filling, such as rainfall, favourable temperatures and luminosity conducive to photosynthesis, plant metabolism, and the synthesis of reserves accumulated in the seeds during maturation (BARROS et al., 2017; CARVALHO; NAKAGAWA, 2012; CRUZ et al., 2020; LAMARCA et al., 2013). In addition to the climate conditions, seed weight can be influenced by the time of the harvest, field management, plant nutrition, competition from weeds, and phytosanitary treatments (LAURA et al., 2009).
When determining the number of seeds of other species, 26 species were identified in the 19 batches under evaluation: two cultivated species (Urochloa sp. and Crotalaria lanceolata), 13 wild invasive species, ten restricted noxious-weed species, and one prohibited noxiousweed species (Table 3). The seeds of other cultivated species, wild invasive species, and restricted and prohibited noxious-weed species were found in 53%, 79%, 89% and 16% of the batches, respectively.
Number of other seeds by cultivated species, wild invasive species, and restricted and prohibited noxious-weed species, in 19 seed batches of P. maximum ‘Mombasa’ from the districts of Auriflama, SP (Aur.), Guzolândia, SP (Guz.), Quirinópolis, GO (Qui.) and Serranópolis, GO (Ser.), 2014/2015 harvest
Seeds of the prohibited noxious species Cyperus sp. (BRASIL, 2008) were found in Batches 1 and 3 from Quirinópolis and Batch 2 from Serranópolis. As such, 25% and 50%, respectively, of the batches from these districts contained prohibited seeds. These batches would be rejected and could not be marketed, due to not meeting the Norms and Standards for the Production and Marketing of Seeds of Tropical Forage Species (BRASIL, 2008).
Noxious seeds are from species that, as they are hard to eradicate in the field or during processing, are harmful to the crop or its product, and are restricted by specific Norms and Standards (BRASIL, 2008, 2009).
According to Annex VI of Normative Instruction no. 30 of 2008, published by MAPA (BRASIL, 2008), batches that contain a maximum of 30 seeds of other cultivated species, 40 seeds of wild invasive species, and 50 seeds of restricted noxious species can be used for marketing.
Therefore, except for the batches that were rejected due to the occurrence of prohibited weed species, the remaining batches would be suitable for marketing, as they did not exceed the maximum number of restricted species allowed by the Norms and Standards for the Production and Marketing of Seeds of Tropical Forage Species (BRASIL, 2008).
Seeds of Cyperus rotundus, found in the batches in the present study, were also found by Borghi et al. (2008) in seed fields for either forage grass or the production of pasture. This species is aggressive and difficult to control (MENDONÇA et al., 2014; SCARIOT et al., 2017).
Seeds of other cultivated species were found in 88%, 33% and 33% of the batches from Quirinópolis, Auriflama and Guzolândia, respectively. None of the batches from Serranópolis contained seeds of other cultivated species. Seeds of wild species were found in all the batches from Guzolândia, 83% of the batches from Auriflama, 75% of the batches from Quirinópolis and 50% of the batches from Serranópolis. The seeds of wild species are common, but easy to control or separate during processing (BRASIL, 2009).
Seeds of restricted noxious weeds were found in 100%, 100%, 88% and 67% of the batches from Auriflama, Serranópolis, Quirinópolis, and Guzolândia, respectively. The presence of weeds in production fields may be related to their easy propagation and to ineffective weed management (ONEN et al., 2018).
To minimise problems with seeds of other species in seed batches of a particular crop, specific weed-management practices should be adopted while still in the field, considering the distribution of the weeds, the fertility of the soil, climate conditions and crop management practices (irrigation, fertilisation, and the use of herbicides) (ONEN et al., 2018).
Processing can be used to completely or partially remove the seeds of weeds and other undesired species that are found in the seed batches (BRASIL, 2011; MELO et al., 2016a, b; SILVA et al., 2019). However, the efficiency of the operation depends on the presence of physical differences between the forage-grass seeds and any seeds that should be removed from the batch (MELO et al., 2016a).
CONCLUSIONS
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The physical quality and viability of seeds of P. maximum ‘Mombasa’ are affected by their provenance;
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All the batches have seeds of sufficient viability to meet marketing standards;
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Based on physical purity, Batch 2 from Quirinópolis should not be marketed.
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Based on an examination of noxious seeds, 85% of the batches are suitable for marketing.
ACKNOWLEDGMENT
This study was carried out with the support of the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil (CAPES) - Finance Code 001.
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Edited by
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Editor-in-Chief: Alek Sandro Dutra - alekdutra@ufc.br
Publication Dates
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Publication in this collection
06 July 2022 -
Date of issue
2022
History
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Received
21 Jan 2020 -
Accepted
10 Feb 2022
