GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN <i>IN VITRO</i>

WELZ, VANESSA FERNANDES FONSECA; TRETTEL, JÉSSICA REZENDE; NASCIMENTO, ANDRESSA BEZERRA; MAGALHÃES, HÉLIDA MARA

doi:10.1590/1983-21252020v33n309rc

VANESSA FERNANDES FONSECA WELZ

Postgraduate Programs in Biotechnology Applied to Agriculture, Universidade Paranaense, Umuarama, PR, Brazil; vanessaferfonseca@gmail.com, jrtrettel@gmail.com, helidamara@prof.unipar.br.

https://orcid.org/0000-0002-3852-5094

JÉSSICA REZENDE TRETTEL

Postgraduate Programs in Biotechnology Applied to Agriculture, Universidade Paranaense, Umuarama, PR, Brazil; vanessaferfonseca@gmail.com, jrtrettel@gmail.com, helidamara@prof.unipar.br.

https://orcid.org/0000-0003-0904-829X

ANDRESSA BEZERRA NASCIMENTO

Graduate Agronomy, Universidade Paranaense, Umuarama, PR, Brazil; andressa.bnascimento@hotmail.com.

https://orcid.org/0000-0002-5327-0368

HÉLIDA MARA MAGALHÃES ^**Corresponding author

Postgraduate Programs in Biotechnology Applied to Agriculture, Universidade Paranaense, Umuarama, PR, Brazil; vanessaferfonseca@gmail.com, jrtrettel@gmail.com, helidamara@prof.unipar.br.

https://orcid.org/0000-0002-0402-7716

*Corresponding author

Figures (2)
Tables (4)

Thumbnail

Figure 1
Isolated effect of growing season and growing medium on abnormal seedlings of Ocimum basilicum 'genovese'.

*Averages followed by the same letter in the column do not differ from each other by Tukey’s test at 5% probability. T1 - 100% MS, absence of regulators and activated charcoal; T2 - 70% MS 0.1mg L^-1 BAP; T3 - 70% MS, activated charcoal 3.0g L^-1 + 0.1mg L^-1 BAP; T4 - 100% MS BAP 0.4 mg L^-1 + 0.2 mg L^-1 NAA; T5 - 100% MS, activated charcoal 3.0 g L^-1 + 0.4 mg L^-1 BAP + 0.2 mg L^-1 NAA. All treatments contained 30.0 g L^-1 sucrose and 6.5 g L^-1 agar. Season 1 - 26 days after seed inoculation; season 2 - 52 days after seed inoculation and season 3 - 80 days after seed inoculation.

Thumbnail

Figure 2
Enzymatic activity of ascorbid peroxidase APX (A), CAT catalase (B) and superoxide dismutase SOD (C) obtained from different culture medium formulations.

*Averages followed by the same letter in the column do not differ from each other by Tukey’s test at 5% probability. T1 - 100% MS, absence of regulators and activated charcoal; T2 - 70% MS 0.1mg L^-1 BAP; T3 - 70% MS, activated charcoal 3.0g L^-1 + 0.1mg L^-1 BAP; T4 - 100% MS BAP 0.4 mg L^-1 + 0.2mg.L^-1 NAA; T5 - 100% MS, activated charcoal 3.0 g L^-1 + 0.4 mg L^-1 BAP + 0.2 mg L^-1 NAA. All treatments contained 30.0 gL^-1 sucrose and 6.5 g L^-1 agar.

Thumbnail

Table 1
Concentration of MS medium, growth regulators and activated charcoal for genovese basil growth.

Thumbnail

Table 2
Number of leaves (NL), number of shoots (NS), length of shoots (LS), fresh shoots (FS), dry shoots (DS), fresh root (FR), dry root (DR), fresh callus (FC), dry callus (DC) and chlorophyll (CL) of seedlings of O. basilicum 'genovese'

Thumbnail

Table 3
Macro and micronutrient constituents, N (Nitrogen), Cu (Copper) Zn (zinc), S (Shoot), R (Root) of O. basilicum 'genovese' in function of different MS medium formulations.

Thumbnail

Table 4
Antioxidant activity obtained from fresh genovese basil leaves grown in vitro.

	Treatment 1	Treatment 2	Treatment 3	Treatment 4	Treatment 5
MS medium (%)	100	70	70	100	100
Activated charcoal (gL^-1)	0.0	0.0	3.0	0.0	3.0
BAP (mgL^-1)	0.0	0.1	0.1	0.4	0.4
NAA (mgL^-1)	0.0	0.0	0.0	0.2	0.2

Treatment	NL	NS	LS (mm)	FS(g)	DS (g)
Tl	14.65± 3.05^ab	2.0± 1.82^a	52.81±6.39^a	3.65 ± 1.43^a	0.27 ± 0.05^a
T2	8.91± 7.41^b	0.0± 0.00^b	49.85± 40.49^a	0.91 ± 0.79^bc	0.05 ± 0.07^b
T3	5.16± 2.72^b	0.0± 0.00^b	52.20± 5.95^a	0.44 ± 0.26^c	0.04 ± 0.01^b
T4	21.93± 9.33^a	1.12±0.85^ab	48.39± 3.61^a	2.56 ± 0.65^ab	0.18 ± 0.07^a
T5	7.97± 0.94^b	0.0± 0.00^b	56.73± 16.02^a	1.04 ± 0.40^bc	0.28 ± 0.34^a
	FR (g)	DR (g)	FC (g)	DC (g)	CL
T1	3.18± 1.03^a	0.14 ± 0.08^b	0.00± 0.00^a	0.00 ± 0.00^a	17.05± 2.72^a*
T2	0.95± 0.66^b	0.6 ± 0.04^abc	0.00± 0.00^a	0.00 ± 0.00^a	15.07± 10.90^a
T3	0.14± 0.05^b	0.01 ± 0.00^c	0.00± 0.00^a	0.00 ± 0.00^a	10.47± 7.29^a
T4	2.81± 0.82^a	0.09 ± 0.02^ab	1.75± 2.05^a	0.10 ± 0.11^a	20.41± 1.52^a
T5	0.25± 0.07^b	0.23 ± 0.01^a	0.00± 0.00^a	0.00 ± 0.00^a	13.94±13.28^a

Treatments	g Kg^-1		mg kg^-1		mg kg^-1
Treatments	N		Cu		Zn
	S	R	S	R	S	R
T1	40.31±0.52	37.39±4.55	10.00±0.00	11.50±2.12	81.00±4.24	83.00±2.82
T2	42.06±0.00	30.55±0.00	10.50±2.12	10.50±2.12	74.00±16.97	60.00±2.82
T3	-	27.90±0.00	10.50±2.12	-	60.00±0.00	-
T4	54.42±2.10	38.75±0.71	15.00±4.24	9.50±0.71	89.50±2.12	53.50±0.70
T5	40.47±2.07	-	10.00±2.83	17.00±0.0	68.00±15.55	55.00±2.82

Treatments	T1	T2	T3	T4	T5
Antioxidant activity (%)	1.94±0.25 ^bc*	4.69±0.80 ^ab	1.86±0.17^bc	0.08 ±0.01^c	6.92±0.91^a

[1] *Corresponding author

Brasil

Brasil

GROWTH, ENZYMATIC ACTIVITY, AND ANTIOXIDANT ACTIVITY OF SWEET BASIL GROWN IN VITRO

CRESCIMENTO, ATIVIDADE ENZIMÁTICA E ANTIOXIDANTE DE MANJERICÃO CULTIVADO IN VITRO

ABSTRACT