Yield, composition and toxicity of piperaceae volatiles to pest insects

Mendonça, Jamila F.; Sousa, Adalberto H. De; Faroni, Lêda R. A.; Fernandes, Carromberth C.; Santos, Ana C. V. dos; Lopes, Lucas M.; Ferraz, Maria S. S.; Prates, Lucas H. F.

doi:10.1590/1983-21252024v3712298rc

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Scientific Article • Rev. Caatinga 37 • 2024 • https://doi.org/10.1590/1983-21252024v3712298rc copy

Yield, composition and toxicity of piperaceae volatiles to pest insects

Rendimento, composição e toxicidade de voláteis de piperáceas para insetos-praga

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The objective of this study was to investigate the influence of leaf drying techniques (bench and oven at 35 and 45 °C) on the essential oil (EO) yield of Piper aduncum L., Piper anonifolium Kunth, Piper crassinervium Kunth and Piper hispidinervum C. DC., and to analyze the chemical profile of EOs and the insecticidal potential of these oils against Ascia monuste orseis (Godart), Atta sexdens L., Zabrotes subfasciatus (Boheman), Cryptolestes ferrugineus (Stephens) and Sitophilus zeamais Motschulsky. EO yield was evaluated using four replicates of 100g of dry leaves. The EOs were obtained by hydrodistillation and subjected to GC-MS analysis to assess the chemical composition. Concentrations of 2.60 and 157.25 nL/cm² were used in the oil toxicity bioassays. EO yield was higher in the species P. aduncum and P. hispidinervum using leaves dried in oven at 45 °C, with average yields of 4.72±0.04% and 2.61±0.26%, respectively. The major constituents present in the EOs of P. hispidinervum and P. aduncum were Safrole (98.80%) and Apiole (90.00%). For P. anonifolium, the major constituents were α-Muurolene (23.11%), y-Muurolene (16.60%) and Cadina-1(10), while for P. crassinervium, they were Viridiflorol (27.70%) and Sabinene (15.50%). It was found that the EOs of P. aduncum, P. anonifolium, P. crassinervium and P. hispidinervum had a toxic effect on insects, except for P. anonifolium and P. crassinervium for S. zeamais. EO yield was higher in the species P. aduncum and P. hispidinervum, and these oils caused a higher mortality rate for the investigated insects.

Keywords:
Piper ; Essential oils; Secondary metabolites; Major compounds; Bioinsecticide

Botanical species	Yield (%) (±M.S.E.)
Botanical species	Bench	35 °C	45 °C
Piper aduncum	3.53±0.25 Ab*	3.36±0.13 Ab	4.72±0.04 Aa
P. anonifolium	0.13±0.01 Ca	0.20±0.01 Ca	0.12±0.01 Ca
P. crassinervium	0.32±0.02 Ca	0.28±0.04 Ca	0.22±0.01 Ca
P. hispidinervum	2.00±0.09 Bb	1.76±0.08 Bb	2.61±0.26 Ba

		----------Relative area (%)²----------
Compound	Class	RT¹ (min.)	P.ad	P.an	P.cr	P.hi
α-Pinene	Monoterpene	6.551	–	–	5.64	–
Sabinene	Monoterpene	8.307	–	–	15.49	–
Limonene	Monoterpene	10.237	–	–	0.95	–
α-Terpineol	Monoterpene	19.011	–	–	0.34	–
Safrole	Phenylpropane	25.113	–	–	–	98.83
δ-Elemene	Sesquiterpene	27.842	–	–	0.40	–
α-Cubebene	Sesquiterpene	28.572	–	1.61	1.33	–
Cyclosativene	Sesquiterpene	29.424	–	5.33	–	–
α-Copaene	Sesquiterpene	30.120	0.36	–	0.66	–
β-Elemene	Sesquiterpene	31.180	0.28	–	7.07	–
α-Gurjunene	Sesquiterpene	32.145	–	–	2.96	–
Caryophillene (Z)	Sesquiterpene	32.710	2.12	5.62	3.36	–
β-Copaene	Sesquiterpene	33.318	–	–	0.57	–
γ-Elemene	Sesquiterpene	33.666	–	–	0.40	–
α-Guaiene	Sesquiterpene	34.635	–	1.39	–	–
α-Humulene	Sesquiterpene	34.744	0.44	–	–	–
β-Humulene	Sesquiterpene	34.752	–	0.46	0.88	–
Alloaromadendrene	Sesquiterpene	34.935	–	0.48	–	–
Isocaryophillene	Sesquiterpene	35.178	–	–	2.22	–
Ishwarane	Sesquiterpene	35.222	–	4.89	–	–
γ-Muurolene	Sesquiterpene	36.265	–	16.60	1.01	–
Germacrene D	Sesquiterpene	36.413	1.62	–	2.61	–
β-Selinene	Sesquiterpene	36.686	–	–	1.22	–
Valencene	Sesquiterpene	36.712	–	3.24	–	–
Ledene	Sesquiterpene	37.251	–	–	2.09	–
Bicyclogemacrene	Sesquiterpene	37.329	0.53	–	–	1.17
β-Curcumene	Sesquiterpene	37.481	–	2.45	–	–
α-Muurolene	Sesquiterpene	37.712	–	23.11	0.74	–
Pentadecane	Sesquiterpene	37.764	0.61	–	–	–
γ-Gurjunene	Sesquiterpene	37.808	–	–	0.68	–
β-Bisabolene	Sesquiterpene	38.207	–	1.87	–	–
γ-Cadinene	Sesquiterpene	38.386	–	–	0.71	–
δ-Cadinene	Sesquiterpene	38.977	–	–	2.00	–
Myristicin	Phenylpropane	38.998	1.70	–	–	–
Cadina-1(10),4-diene	Sesquiterpene	39.020	–	11.20	–	–
α-Calacorene	Sesquiterpene	40.050	–	0.65	–	–
Germacrene B	Sesquiterpene	40.754	–	–	0.43	–
Dillapiole	Phenylpropane	40.810	0.57	–	–	–
Nerolidol (E)	Sesquiterpene	41.454	–	–	4.75	–
Spathulenol	Sesquiterpene	42.006	0.35	–	0.49	–
Caryophyllene oxide	Sesquiterpene	42.266	0.35	–	–	–
Globulol	Sesquiterpene	42.345	–	–	2.10	–
Guaiol	Sesquiterpene	42.770	1.09	–	–	–
Viridiflorol	Sesquiterpene	42.845	–	–	27.74	–
Cedrol	Sesquiterpene	44.735	–	2.53	–	–
Cubenol	Sesquiterpene	44.857	–	–	1.43	–
Muurola-4,10(14)-dien-1β-ol	Sesquiterpene	44.913	–	7.28	–	–
Epicubenol	Sesquiterpene	45.074	–	0.94	–	–
Apiole	Phenylpropane	45.122	90.00	–	–	–
Torreyol	Sesquiterpene	45.904	–	4.10	0.92	–
Epi-α-cadinol	Sesquiterpene	46.308	–	5.16	2.59	–
α-Cadinol	Sesquiterpene	46.326	–	–	5.35	–
Himachalol	Phenylpropane	47.108	–	–	0.41	–
β-Copaen-4α-ol	Sesquiterpene	47.125	–	0.58	–	–
Cembrene	Diterpene	47.347	–	–	0.45	–
Isolongifolan-7α-ol	Sesquiterpene	47.599	–	0.26	–	–
Ylangenol	Sesquiterpene	49.250	–	0.24	–	–
Monoterpenes					14%
Sesquiterpenes			77%	100%	80%	50%
Phenylpropanoids			23%		3%	50%
Total			100%	100%	97%	100%

Universidade Federal Rural do Semi-Árido Avenida Francisco Mota, número 572, Bairro Presidente Costa e Silva, Cep: 5962-5900, Telefone: 55 (84) 3317-8297 - Mossoró - RN - Brazil
E-mail: caatinga@ufersa.edu.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] *Corresponding author: <adalberto.sousa@ufac.br>