Immunohistochemical evaluation of fibrillar components of the
          extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men
          with inguinal hernia

Gonçalves, Rogério De Oliveira; De Moraes e Silva, Evandro; Lopes Filho, Gaspar De Jesus

doi:10.1590/S0100-69912014000100006

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Original Articles • Rev. Col. Bras. Cir. 41 (01) • Jan-Feb 2014 • https://doi.org/10.1590/S0100-69912014000100006 copy

Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal hernia

Authorship SCIMAGO INSTITUTIONS RANKINGS

OBJECTIVE:

to evaluate the role of fibrillar extracellular matrix components in the pathogenesis of inguinal hernias.

METHODS:

samples of the transverse fascia and of the anterior sheath of the rectus abdominis muscle were collected from 40 men aged between 20 and 60 years with type II and IIIA Nyhus inguinal hernia and from 10 fresh male cadavers (controls) without hernia in the same age range. The staining technique was immunohistochemistry for collagen I, collagen III and elastic fibers; quantification of fibrillar components was performed with an image analysis processing software.

RESULTS:

no statistically significant differences were found in the amount of elastic fibers, collagen I and collagen III, and the ratio of collagen I / III among patients with inguinal hernia when compared with subjects without hernia.

CONCLUSION:

the amount of fibrillar extracellular matrix components did not change in patients with and without inguinal hernia.

Immunohistochemistry; Collagen; Extracellular matrix; Fascia; Hernia, inguinal

Age range	Nyhus type	IIIndirect hernia	Nyhus type IIIADirect hernia		Controlwithout	hernia
(in years)	(number)	%	(number)	%	(number)	%
20 a 30	10	50%	1	5%	7	70%
31 a 40	2	10%	4	20%	1	10%
41 a 50	4	20%	6	30%	1	10%
51 a 60	4	20%	9	45%	1	10%
Total	20	100%	20	100%	10	100%

Colagen	G r o u p	n	Average	Standard	Deviation Minimum	Maximum
Total	50	1.2	0.6	0.5	3.3
Transversalis Fascia	Indirect hernia	20	1.2	0.5	0.5	2.4
	Direct hernia	20	1.3	0.7	0.5	3.3
	Control	10	1.1	0.3	0.6	1.8
	Kruskal-Wallis test: p-valor=0.648
	Total	50	1.2	0.5	0.4	3.6
Sheath of the Rectus Abdominis Indirect hernia		20	1.3	0.7	0.4	3.6
	Direct hernia	20	1.1	0.3	0.5	1.7
	Control	10	1.1	0.3	0.7	1.7

Fiber	G r o u p	n	Average	Standard Deviation	Minimum	Maximum
Collagen fiber type I	Total	50	21.3	6.1	10.2	35.2
(Transversalis Fascia)	With hernia	40	20.8	5.9	10.2	35.2
	Control	10	23.5	6.4	15.2	34.3
	p=0 . 254
Collagen fiber type I	Total	50	25.1	8.2	7.7	47.9
(Sheath of the Rectus Abdominis)	With hernia	40	24.4	8.5	7.7	47.9
	Control	10	27.7	6.6	18.6	39.4
	p=0 . 296
Collagen fiber type III	Total	50	19.8	7.6	3.3	40.4
(Transversalis Fascia)	With hernia	40	19.0	8.0	3.3	40.4
	Control	10	22.8	4.9	16.7	31.6
	p=0 . 326
Collagen fiber type III	Total	50	22.4	7.2	9.8	43.5
(Sheath of the Rectus Abdominis)	With hernia	40	21.6	7.1	9.8	43.5
	Control	10	25.8	7.0	17.5	42.0
	p=0 . 426
Elastic fiber	Total	50	15.6	5.3	4.6	27.6
(Transversalis Fascia)	With hernia	40	15.8	5.8	4.6	27.6
	Control	10	14.9	3.4	9.8	20.5
	p=0 . 676
Elastic fiber	Total	50	15.3	4.7	7.5	28.5
(Sheath of the Rectus Abdominis)	With hernia	40	15.5	4.4	7.5	28.5
	Control	10	14.6	5.9	7.6	27.8
	p=0 . 344

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[1] Address for correspondence: Rogério de Oliveira Gonçalves E-mail: r.gov@uol.com.br