BACKGROUND: To evaluate, through pregnancy and pressure burst tests, the effectiveness of sheep uterine tube sterilization through hysteroscopic insertion of cyanoacrylate adhesive. METHODS: Adult sheep (n=12), with one previous pregnancy, were distributed as follows: PO Group (n=3) submitted to laparotomy and Pomeroy surgical procedure of tubal obstruction, Group SH (n=3) submitted to hysteroscopy application of saline solution inside the tubal isthmus, and Group AD (n=6) submitted to hysteroscopy application of 0.5 mL of n-2-butil-cyanoacrylate inside the tubal isthmus. They were allowed to copulate with fertile males during ninety days. The non pregnant sheep, at the 90th day, were submitted to laparotomy, the uterine tubes were removed and their diameters analyzed. The uterine tubes were evaluated for patency with methylene blue dye injection. The overpressure burst test (80mmHg) was then applied through the uterine cervix. Data were evaluated by t Student and Fisher test. RESULTS: All sheep from groups PO and AD did not get pregnant (0%) in contrast with sheep from group SH (100%); the adhesive remained stable inside the tubal lumen. The diameter of the proximal tube in AD group (0.650 ± 0.16cm) was significantly wider than the PO group (0.195 ± 0.04* cm) (p= 0.01). The overpressure burst and dye patency tests were negative for all animals from PO group and only one animal in AD group was positive for the pressure burst test. CONCLUSION: The hysteroscopic insertion of cyanoacrylate inside the tubal lumen of the sheep was effective to obstruct the uterine tube and to promote the sterilization, and would be an option for future applying in human beings.
Fallopian tubes; Hysteroscopy; Sterilization, reproductive; Tissue adhesives; Sheep
